Effects of Rumen Undegradable Unsaturated Fat on Growth Performance, Biochemical Indices and Related Gene Expression of Angus Beef
YANG Zhiling1, YANG Guoyi2, LIU Qi2, HE Lei2, WU Shufeng1, YANG Wenqiang1, ZHANG Shuanlin1, LIU Qiang1, GUO Gang1
1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. General Station of Grassland, Bureau of Forestry and Grassland of Shanxi Province, Taiyuan 030000, China
Abstract：The objective of the current study was to evaluate the effects of rumen undegradable unsaturated fat (RUF) on growth performance, biochemical indices and related gene expression of Angus beef. Twenty-four Angus steers[average body weight of (447.78±2.53) kg, 15 to 17 months of age] were randomly assigned to 4 groups with 6 beef in each group. Groups 1, 2, 3 and 4 were fed experimental diets containing 0, 2.0%, 4.0% and 6.0% RUF, respectively. The experiment lasted 77 days including a 10-day preliminary test period and a 67-day formal test period, and the formal test period was divided into two stages:early stage (27 days) and later stage (40 days). The results showed as follows:1) in early stage, the average daily gain of group 4 was significantly higher than that of groups 1 and 2 (P<0.05), the ratio of feed to gain of group 1 was significantly higher than that of group 4 (P<0.05). In early stage, later stage and whole stage, there was no significant difference on the average daily feed intake among all groups (P>0.05). 2) In early stage and later stage, the serum insulin (INS) content of groups 2 and 3 was significantly higher than that of group 1 (P<0.05), the serum glucagon (GC) content of groups 2, 3 and 4 was significantly higher than that of group 1 (P<0.05). In early stage, the serum growth hormone (GH) content of group 3 was significantly lower than that of group 1 (P<0.05); in later stage, the serum GH content of groups 2, 3 and 4 was significantly lower than that of group 1 (P<0.05). 3) In early stage and later stage, there were no significant differences on the activities of adipose triglyceride lipase (ATGL), carnitine palmitoyl transferase-Ⅰ (CPT-Ⅰ) and acetyl-coenzyme A carboxylase (AACase) in serum among all groups (P>0.05). 4) In early stage and later stage, there were no significant differences on the glucose (GLU), triglyceride (TG), albumin (ALB) and total cholesterol (TCHO) contents and alanine aminotransferase (ALT) activity in serum among all groups (P>0.05). In early stage, the serum total protein (TP) content of group 4 was significantly higher than that of group 1 (P<0.05), the serum malondialdehyde (MDA) content of groups 2, 3 and 4 was significantly lower than that of group 1 (P<0.05), the serum glutamic oxaloacetic transaminase (AST) activity of groups 2, 3 and 4 was significantly higher than that of group 1 (P<0.05); in later stage, the serum (MDA content of groups 3 and 4 was significantly lower than that of group 1 (P<0.05), the serum AST activity of groups 2 and 3 was significantly higher than that of group 1 (P<0.05). 5) The gene expression of peroxisome proliferator-activated receptor γ (PPARγ) in Longissimus dorsi of group 4 was significantly higher than that of group 1 (P<0.05). In conclusion, dietary RUF has no significant effects on the average daily gain, average daily feed intake and ratio of feed to gain of beef in whole stage, and has no significant effects on the activities of aATGL, CPT-Ⅰ and AACase in serum, but improves the contents of GC and INS in serum, promotes the gene expression of PPARγ in Longissimus dorsi. Overall consideration, under this experimental conditions, the optimum dietary RUF content is 4.0%.
杨致玲, 杨国义, 刘旗, 赫磊, 吴树峰, 杨文强, 张拴林, 刘强, 郭刚. 过瘤胃不饱和脂肪对安格斯肉牛生长性能、血清指标及相关基因表达的影响[J]. 动物营养学报, 2019, 31(8): 3632-3642.
YANG Zhiling, YANG Guoyi, LIU Qi, HE Lei, WU Shufeng, YANG Wenqiang, ZHANG Shuanlin, LIU Qiang, GUO Gang. Effects of Rumen Undegradable Unsaturated Fat on Growth Performance, Biochemical Indices and Related Gene Expression of Angus Beef. Chinese Journal of Animal Nutrition, 2019, 31(8): 3632-3642.
DUCKETT S K,GILLIS M H.Effects of oil source and fish oil addition on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets[J].Journal of Animal Science,2010,88(8):2684-2691.
[8]
LADEIRA M M,SANTAROSA L C,CHIZZOTTI M L,et al.Fatty acid profile,color and lipid oxidation of meat from young bulls fed ground soybean or rumen protected fat with or without monensin[J].Meat Science,2014,96(1):597-605.
[18]
冯仰廉.肉牛营养需要和饲养标准[M].北京:中国农业大学出版社,2000:23.
[28]
RIO D C,ARES M,Jr,HANNON G J et al.Purification of RNA using TRIzol (TRI reagent)[J].Cold Spring Harbor Protocois,2010(6),doi:10.1101/pdb.prot5439.
[11]
SIMONS K,TOOMRE D.Lipid rafts and signal transduction[J].Nature Reviews Molecular Cell Biology,2000,1(1):31-39.
DUCKETT S K,GILLIS M H.Effects of oil source and fish oil addition on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets[J].Journal of Animal Science,2010,88(8):2684-2691.
[9]
Oliveira D M,LADEIRA M M,CHIZZOTTI M L,et al.Fatty acid profile and qualitative characteristics of meat from Zebu steers fed with different oilseeds[J].Journal of Animal Science,2011,89(8):2546-2555.
[13]
KUHN H,BANTHIYA S,VAN LEYEN K.Mammalian lipoxygenases and their biological relevance[J].Biochimica et Biophysica Acta:Molecular and Cell Biology of Lipids,2015,1851(4):308-330.
LONG N M,BURNS T A,DUCKETT S K,et al.Reproductive performance and serum fatty acid profiles of underdeveloped beef heifers supplemented with saturated or unsaturated rumen bypass fat compared to an isocaloric control[J].The Professional Animal Scientist,2014,30(5):502-509.
[33]
SIEMS W G,GRUNE T,ESTERBAUER H.4-Hydroxynonenal formation during ischemia and reperfusion of rat small intestine[J].Life Science,1995,57(8):785-789.
[36]
SMITH S,WITKOWSKI A,JOSHI A K.Structural and functional organization of the animal fatty acid synthase[J].Progress in Lipid Research,2003,42(4):289-317.
[10]
SCOLLAN N,JEAN-FRANÇOIS H,NUERNBERG K,et al.Innovations in beef production systems that enhance the nutritional and health value of beef lipids and their relationship with meat quality[J].Meat Science,2006,74(1):17-33.
[12]
STILLWELL W,WASSALL S R.Docosahexaenoic acid:membrane properties of a unique fatty acid[J].Chemistry and Physics of Lipids,2003,126(1):1-27.
[13]
KUHN H,BANTHIYA S,VAN LEYEN K.Mammalian lipoxygenases and their biological relevance[J].Biochimica et Biophysica Acta:Molecular and Cell Biology of Lipids,2015,1851(4):308-330.
LONG N M,HILL G M,BAKER J F,et al.Reproductive performance of beef heifers supplemented with corn gluten feed and rumen-protected fat before breeding[J].The Professional Animal Scientist,2007,23(4):316-324.
[33]
SIEMS W G,GRUNE T,ESTERBAUER H.4-Hydroxynonenal formation during ischemia and reperfusion of rat small intestine[J].Life Science,1995,57(8):785-789.
[36]
SMITH S,WITKOWSKI A,JOSHI A K.Structural and functional organization of the animal fatty acid synthase[J].Progress in Lipid Research,2003,42(4):289-317.
[35]
WILLSON T M,WAHLI W.Peroxisome proliferator-activated receptor agonists[J].Current Opinion in Chemical Biology,1997,1(2):235-241.
[37]
ROY R,GAUTIER M,HAYES H,et al.Assignment of the fatty acid synthase (FASN) gene to bovine chromosome 19(19q22) by in situ hybridization and confirmation by somatic cell hybrid mapping[J].Cytogenetics and cell Genetics,2001,93(1/2):141-142.
