Abstract:This experiment was conducted to evaluate the effects of Pediococcus acidilactici on growth performance, fecal microbiota, serum biochemical and immune indexes of growing pigs. A total of 140 "Landrace×Yorkshire" crossbred growing pigs with an average body weight of (23.21±0.84) kg were divided into 2 groups: control group and experimental group. Each group had 5 replicates with 14 pigs per replicate. Pigs in the control group were fed a basal diet, and those in the experimental group were fed the basal diet supplemented with 2.70×109 CFU/kg Pediococcus acidilactici freeze-drying preparation. The adjustment period was 5 d, and the experiment period was 34 d. The results showed that compared with the control group: 1) the average daily gain (ADG) of growing pigs was increased by 5.64% (P<0.05), and the feed/gain (F/G) was decreased by 4.62% (P<0.05) in the experimental group; 2) the number of fecal lactic acid bacteria of growing pigs was significantly increased (P<0.05), and the numbers of Escherichia coli and Staphylococcus aureus were significantly decreased (P<0.05) in the experimental group; 3) Pediococcus acidilactici increased the fecal microbiota species richness, the proportions of Firmicutes, Prevotella, Megasphaera and Lactobacillus were also increased in the experimental group; 4) the contents of serum total protein (TP), globulin (GLB), immunoglobulin G (IgG) and immunoglobulin A (IgA) were increased by 13.45% (P<0.05), 14.91% (P<0.05), 20.91% (P<0.05) and 44.00% (P<0.05), and the contents of serum urea nitrogen (UN) and haptoglobin (HP) were decreased by 19.46% (P<0.05) and 38.71% (P<0.05) in the experimental group, respectively. The results indicate that dietary supplemented with 2.70×109 CFU/kg Pediococcus acidilactici can improve the intestinal microbiota composition, enhance the immune function and improve the growth performance of growing pigs.
刘辉, 季海峰, 王四新, 张董燕, 王晶, 张伟, 王雅民. 乳酸片球菌对生长猪生长性能、粪便菌群、血清生化和免疫指标的影响[J]. 动物营养学报, 2020, 32(6): 2558-2566.
LIU Hui, JI Haifeng, WANG Sixin, ZHANG Dongyan, WANG Jing, ZHANG Wei, WANG Yamin. Effects of Pediococcus acidilactici on Growth Performance, Fecal Microbiota, Serum Biochemical and Immune Indexes of Growing Pigs. Chinese Journal of Animal Nutrition, 2020, 32(6): 2558-2566.
MCCANN K S.The diversity-stability debate[J].Nature,2000,405(6783):228-233.
[29]
HU J,NIE Y F,CHEN J W,et al.Gradual changes of gut microbiota in weaned miniature piglets[J].Frontiers in Microbiology,2016,7:1727.
[39]
GILL H S.Stimulation of the immune system by lactic cultures[J].International Dairy Journal,1998,8(5/6):535-544.
[24]
PARK S J,KIM J,LEE J S,et al.Characterization of the fecal microbiome in different swine groups by high-throughput sequencing[J].Anaerobe,2014,28:157-162.
[25]
LAMENDELLA R,DOMINGO J W S,GHOSH S,et al.Comparative fecal metagenomics unveils unique functional capacity of the swine gut[J].BMC Microbiology,2011,11:103.
[3]
PLUSKE J R.Feed- and feed additives-related aspects of gut health and development in weanling pigs[J].Journal of Animal Science and Biotechnology,2013,4:1.
ZHAO W J,WANF Y P,LIU S Y,et al.The dynamic distribution of porcine microbiota across different ages and gastrointestinal tract segments[J].PLoS One,2015,10(2):e0117441.
[26]
VAN DER MEULEN R,MAKRAS L,VERBRUGGHE K,et al.In vitro kinetic analysis of oligofructose consumption by Bacteroides and Bifidobacterium spp. indicates different degradation mechanisms[J].Applied and Environmental Microbiology,2006,72(2):1006-1012.
[33]
MENG Q W,YAN L,AO X,et al.Influence of probiotics in different energy and nutrient density diets on growth performance,nutrient digestibility,meat quality,and blood characteristics in growing-finishing pigs[J].Journal of Animal Science,2010,88(10):3320-3326.
[36]
SZABÓ I,WIELER L H,TEDIN K,et al.Influence of a probiotic strain of Enterococcus faecium on Salmonella enterica serovar Typhimurium DT104 infection in a porcine animal infection model[J].Applied and Environmental Microbiology,2009,75(8):2621-2628.
[2]
ZHANG D Y,JI H F,LIU H,et al.Changes in the diversity and composition of gut microbiota of weaned piglets after oral administration of Lactobacillus or an antibiotic[J].Applied Microbiology and Biotechnology,2016,100(23):10081-10093.
[5]
GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.
[7]
LIU H,WANG S X,ZHANG D Y,et al.Effects of dietary supplementation with Pediococcus acidilactici ZPA017 on reproductive performance,fecal microbial flora and serum indices in sows during late gestation and lactation[J].Asian-Australasian Journal of Animal Sciences,2020,33(1):120-126.
[10]
LOH T C,VAN THU T,FOO H L,et al.Effects of different levels of metabolite combination produced by Lactobacillus plantarum on growth performance,diarrhoea,gut environment and digestibility of postweaning piglets[J].Journal of Applied Animal Research,2013,41(2):200-207.
[20]
LU X M,LU P Z,ZHANG H.Bacterial communities in manures of piglets and adult pigs bred with different feeds revealed by 16S rDNA 454 pyrosequencing[J].Applied Microbiology and Biotechnology,2014,98(6):2657-2665.
[30]
SHAN H N,COLLINS D M.Prevotella,a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides[J].International Journal of Systematic Bacteriology,1990,40(2):205-208.
[1]
DOWARAH R,VERMA A K,AGARWAL N,et al.Effect of swine based probiotic on performance,diarrhoea scores,intestinal microbiota and gut health of grower-finisher crossbred pigs[J].Livestock Science,2017,195:74-79.
[8]
CANIBE N,JENSEN B B.Fermented and nonfermented liquid feed to growing pigs:effect on aspects of gastrointestinal ecology and growth performance[J].Journal of Animal Science,2003,81(8):2019-2031.
[18]
DELZENNE N M,CANI P D.Interaction between obesity and the gut microbiota:relevance in nutrition[J].Annual Review of Nutrition,2011,31(1):15-31.
[28]
FALAGAS M E,SIAKAVELLAS E.Bacteroides,Prevotella,and Porphyromonas species:a review of antibiotic resistance and therapeutic options[J].International Journal of Antimicrobial Agents,2000,15(1):1-9.
[9]
QIAO J Y,LI H H,WANG Z X,et al.Effects of Lactobacillus acidophilus dietary supplementation on the performance,intestinal barrier function,rectal microflora and serum immune function in weaned piglets challenged with Escherichia coli lipopolysaccharide[J].Antonie van Leeuwenhoek,2015,107(4):883-891.
[15]
ROSELLI M,FINAMORE A,BRITTI M S,et al.Alternatives to in-feed antibiotics in pigs:evaluation of probiotics,zinc or organic acids as protective agents for the intestinal mucosa.A comparison of in vitro and in vivo results[J].Animal Reasearch,2005,54(3):203-218.
[17]
CAI C J,CAI P P,HOU C L,et al.Administration of Lactobacillus fermentum I5007 to young piglets improved their health and growth[J].Journal of Animal and Feed Sciences,2014,23(3):222-227.
TURNBAUGH P J,LEY R E,MAHOWALD M A,et al.An obesity-associated gut microbiome with increased capacity for energy harvest[J].Nature,2006,444(7122):1027-1031.
[32]
LOUIS P,HOLD G L,FLINT H J.The gut microbiota,bacterial metabolites and colorectal cancer[J].Nature Reviews Microbiology,2014,12(10):661-672.
[34]
PETERSEN H H,NIELSEN J P,HEEGAARD P M.Application of acute phase protein measurements in veterinary clinical chemistry[J].Veterinarian Research,2004,35(2):163-187.
[35]
AI W Q,YUE Y,XIONG S D,et al.Enhanced protection against pulmonary mycobacterial challenge by chitosan-formulated polyepitope gene vaccine is associated with increased pulmonary secretory IgA and gamma-interferon+T cell responses[J].Microbiology and Immunology,2013,57(3):224-235.
[37]
DONG X L,ZHANG N F,ZHOU M,et al.Effects of dietary probiotics on growth performance,faecal microbiota and serum profiles in weaned piglets[J].Animal Production Science,2013,54(5):616-621.
[11]
COLLINGTON G K,PARKER D S,ARMSTRONG D G.The influence of inclusion of either an antibiotic or a probiotic in the diet on the development of digestive enzyme activity in the pig[J].British Journal of Nutrition,1990,64(1):59-70.
[21]
HILDEBRAND F,NGUYEN T L A,BRINKMAN B,et al.Inflammation-associated enterotypes,host genotype,cage and inter-individual effects drive gut microbiota variation in common laboratory mice[J].Genome Biology,2013,14:R4.
[31]
FUKUDA S,TOH H,HASE K,et al.Bifidobacteria can protect from enteropathogenic infection through production of acetate[J].Nature,2011,469(7331):543-547.