乳铁蛋白对大河乌猪繁殖性能及母猪与仔猪铁营养、血细胞参数、免疫指标和猪β防御素基因表达的影响

doi:10.3969/j.issn.1006-267x.2020.07.018

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摘要本试验旨在研究乳铁蛋白（LF）对大河乌猪繁殖性能及母猪与仔猪铁营养、血细胞参数、免疫指标和猪β防御素（pBD）基因表达的影响。选用经产（3~4胎）、产仔数和体重相近的大河乌猪母猪60头，随机分为5组，每组12个重复，每重复1头母猪。从妊娠第80天至分娩，对照组饲喂基础饲粮，LF1组、LF2组、LF3组分别在基础饲粮中添加100、200、300 mg/kg LF，甘氨酸螯合铁（Fe-Gly）组在基础饲粮中添加500 mg/kg Fe-Gly。考察母猪繁殖性能，检测母猪和仔猪血液红细胞计数（RBC）、血红蛋白（HGB）含量、血清免疫指标以及母猪血清和乳汁铁含量，并检测仔猪肝脏、脾脏和十二指肠pBD基因表达量。结果表明：1）与对照组相比，LF2组仔猪初生重及初生窝重显著提高（P<0.05），LF1和LF3组仔猪初生窝重显著提高（P<0.05）。2）与对照组相比，LF2、LF3和Fe-Gly组母猪血液RBC及HGB含量显著提高（P<0.05），LF1、LF2、LF3和Fe-Gly组仔猪血液RBC及HGB含量显著提高（P<0.05）。3）与对照组相比，LF1、LF2和LF3组母猪血清和乳汁铁含量显著提高（P<0.05）。4）与对照组相比，LF1、LF2和LF3和Fe-Gly组仔猪血清乳酸脱氢酶（LDH）活性显著降低（P<0.05），血清一氧化氮合酶（NOS）活性显著提高（P<0.05）；LF1、LF2组仔猪血清免疫球蛋白G（IgG）含量和溶菌酶（LSZ）活性显著提高（P<0.05）。5）与对照组相比，LF2组仔猪肝脏、脾脏、十二指肠pBD-1基因，肝脏、脾脏pBD-2基因以及脾脏pBD-3基因表达量显著提高（P<0.05）；LF1组仔猪肝脏pBD-1、pBD-2基因以及LF3组仔猪肝脏、脾脏pBD-1基因表达量显著提高（P<0.05）；LF1、LF2、LF3和Fe-Gly组仔猪十二指肠pBD-2基因以及肝脏、十二指肠pBD-3基因表达量显著降低（P<0.05）。由此可见，在大河乌猪妊娠后期饲粮中添加适宜剂量（200 mg/kg）LF可提高仔猪初生重及初生窝重，改善仔猪铁营养和提高血清免疫指标，增加仔猪肝脏、脾脏和十二指肠pBD-1基因表达量，有利于提高仔猪的抗病能力。

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	刘水灵
	安清聪
	郭荣富

关键词 ：乳铁蛋白, 大河乌猪, 繁殖性能, 血红蛋白, 猪&beta, 防御素

Abstract：This experiment was conducted to investigate the effects of effects of lactoferrin(LF) on reproductive performance of Dahe black sow and iron nutrition, blood cell parameters, immune indexes and porcine β-defensin (pBD) gene expression of sows and piglets. Sixty multiparity (3 to 4 fetuses) Dahe black sows with similar litter size and body weight were randomly divided into 5 groups with 12 replicates in each group and 1 sow in each replicate. From the 80th day of gestation to the delivery, sows the control group were fed a basal diet, sows in the LF1, LF2 and LF3 groups were fed the basal diets supplemented with 100, 200 and 300 mg/kg LF, respectively, and sows in the iron glycine chelate (Fe-Gly) were fed the basal diet supplemented with 500 mg/kg Fe-Gly. The reproductive performance of sows was examined, the red blood cell count (RBC) and hemoglobin (HGB) content in blood, serum immune indexes, sow serum and milk iron content were measured, and the expression level of pBD gene in liver, spleen and duodenal of piglets were detected. The results showed as follows:1) compared with the control group, the weight at birth and litter weight at birth of piglets of LF2 group were significantly increased (P<0.05), the litter weight at birth of piglets of LF1 and LF3 groups was significantly increased (P<0.05). 2) Compared with the control group, the blood RBC and HGB content of sows of LF2, LF3 and Fe-Gly groups were significantly increased (P<0.05), and the blood RBC and HGB content of piglets of LF1, LF2, LF3 and Fe-Gly groups were significantly increased (P<0.05). 3) Compared with the control group, the contents of iron in serum and milk of sows of LF1, LF2 and LF3 groups were significantly increased (P<0.05). 4) Compared with the control group, the serum lactate dehydrogenase (LDH) activity of piglets of LF1, LF2 and LF3 groups and Fe-Gly groups was significantly decreased (P<0.05), and the serum nitric oxide synthase (NOS) activity was significantly increased (P<0.05); the serum immunoglobulin G (IgG) content and lysozyme (LSZ) activity of piglets of LF1 and LF2 groups were significantly increased (P<0.05). 5) Compared with the control group, the expression levels of pBD-1 gene in liver, spleen and duodenum, pBD-2 gene in liver and spleen and pBD-3 gene in spleen of piglets of LF2 group were significantly increased (P<0.05); the expression levels of pBD-1 and pBD-2 genes in liver of piglets of LF1 group and pBD-1 in liver and spleen of piglets of LF3 group were significantly increased (P<0.05); and the expression levels of pBD-2 gene in duodenum and pBD-3 gene in liver and duodenum of piglets of LF1, LF2, LF3 and Fe-Gly groups were significantly decreased (P<0.05). In conclusion, dietary adding appropriate dose (200 mg/kg) LF of Dahe black sow in the late pregnancy can increase the weight at birth and litter weight at birth of piglets, improve the iron nutrition and serum immune indexes of piglets, increase the expression levels of pBD-1 gene in liver, spleen and duodenum, which is beneficial to improve the disease resistance of piglets.

Key words： lactoferrin Dahe black sows reproductive performance hemoglobin porcine β-defensin

收稿日期: 2020-01-22

PACS:

S825

基金资助:云南省科技厅重大科技专项子课题"种猪生产营养饲料技术集成与示范"（2012ZAO18-3）与"乳铁蛋白对新生期仔猪肠道健康的影响"（A3007495）

通讯作者: 安清聪,副教授,E-mail:naccynaccy@163.com;郭荣富,教授,博士生导师,E-mail:rongfug@163.com E-mail: naccynaccy@163.com;rongfug@163.com

作者简介: 车丽涛(1979-),女,云南昆明人,博士研究生,从事猪的营养与免疫研究。E-mail:272320726@qq.com

引用本文:

车丽涛, 李岑曦, 徐娜娜, 张春勇, 刘水灵, 安清聪, 郭荣富. 乳铁蛋白对大河乌猪繁殖性能及母猪与仔猪铁营养、血细胞参数、免疫指标和猪β防御素基因表达的影响[J]. 动物营养学报, 2020, 32(7): 3089-3098.
CHE Litao, LI Cenxi, XU Nana, ZHANG Chunyong, LIU Shuiling, AN Qingcong, GUO Rongfu. Effects of Lactoferrin on Reproductive Performance of Dahe Black Sows and Iron Nutrition, Blood Cell Parameters, Immune Indexes and Porcine β-Defensin Gene Expression of Sows and Piglets. Chinese Journal of Animal Nutrition, 2020, 32(7): 3089-3098.

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http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2020.07.018 或 http://www.chinajan.com/CN/Y2020/V32/I7/3089

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