Effects of Dandelion Polysaccharide on Antioxidant Enzyme Activity and Related Gene Expression in Mice
KANG Wenjin1, XU Xingjun1,2, LIU Jiaren1, ZHANG Weiwei1,2, SHAO Shuli1,2
1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161006, China;
2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161007, China
Abstract：To investigate the effects of dandelion polysaccharide on antioxidant enzyme activity and related gene expression in mice, 48 specific pathogen free (SPF) ICR 5-week-old male mice were randomly divided into control group, high concentration dandelion polysaccharide group [600 mg/(kg·d)], medium concentration dandelion polysaccharide group [300 mg/(kg·d)] and low concentration dandelion polysaccharide group [150 mg/(kg·d)]. Dandelion polysaccharide group was given different concentrations of dandelion polysaccharide once a day by regular gavage, while the control group was given the same amount of normal saline for 4 weeks. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities, total antioxidant capacity (T-AOC) and malonaldehyde (MDA) content were determined in the serum and liver tissues of mice, and the relative mRNA expression levels of copper and zinc superoxide dismutase (Cu-ZnSOD), manganese superoxide dismutase (Mn-SOD), glutathione peroxidase-1 (GPX-1) and glutathione peroxidase-4 (GPX-4) in liver of mice were determined by qRT-PCR. The results showed that after gavaged with dandelion polysaccharide, the activities of GSH-Px, SOD and T-AOC in mice serum and liver increased significantly (P<0.05), the content of MDA reduced significantly (P<0.05), and the mRNA relative expression levels of Cu-ZnSOD, Mn-SOD, GPX-1 and GPX-4 were up-regulated significantly (P<0.05). Dandelion flavonoids can significantly improve the antioxidant capacity of mice and have anti-aging effects.
康文锦, 徐兴军, 刘佳人, 张伟伟, 邵淑丽. 蒲公英多糖对小鼠体内抗氧化酶活性及相关基因表达的影响[J]. 动物营养学报, 2020, 32(12): 5910-5915.
KANG Wenjin, XU Xingjun, LIU Jiaren, ZHANG Weiwei, SHAO Shuli. Effects of Dandelion Polysaccharide on Antioxidant Enzyme Activity and Related Gene Expression in Mice. Chinese Journal of Animal Nutrition, 2020, 32(12): 5910-5915.
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