Abstract：The metabolism of bovine mammary epithelial cells (BMECs) is vigorous during lactation, which leads to the production of reactive oxygen species (ROS) and the occurrence of oxidative stress. Moringa leaf polysaccharide (MLP) has the ability of scavenging active oxygen and free radicals, however, whether it has the potential to alleviate oxidative damage of mammary epithelial cells in dairy cows is still unknown. Therefore, MLP was used as an additive in this study to evaluate its protective effect on the oxidative damage of BMECs induced by hydrogen peroxide (H2O2). In this experiment, BMECs were first cultured in different concentrations of H2O2 for 2 h to establish an oxidative stress model to determine the optimal concentration of H2O2. Subsequently, BMECs were cultured in MLP solution with different concentrations for 2 h to determine the optimal concentration of MLP. Finally, H2O2 with a concentration of 500 μmol/L and MLP with a concentration of 4 mg/mL were selected for the following experiments. The experiment was divided into four groups: CON1 (BMECs), CON2 (BMECs+H2O2), injury group (BMECs+H2O2) and protection group (BMECs+MLP+H2O2), and three repeats in each group. The number of reactive oxygen species (ROS) in BMECs, apoptosis of BMECs, catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and malondialdehyde (MDA) content in BMECs were detected. The results showed as follows: the results of ROS detection showed that MLP inhibited the production of ROS in cells. The results of Hochest33258 staining and transmission electron microscopy showed that MLP reduced the apoptotic rate of BMECs, meanwhile, maintaining the integrity of cell membrane and cell structure. The results of kits test showed that MLP increased the activities of CAT, GSH-Px and SOD, and decreased the content of MDA. Overall, the MLP can effectively slow down the apoptosis of mammary epithelial cells and improve their antioxidant capacity.
马广明, 刘骥, 姜鑫, 张永根. 辣木叶多糖对过氧化氢诱导奶牛乳腺上皮细胞氧化损伤的保护作用[J]. 动物营养学报, 2021, 33(1): 350-358.
MA Guangming, LIU Ji, JIANG Xin, ZHANG Yonggen. Protective Effects of Moringa Leaf Polysaccharide on Oxidative Damage of Dairy Cow Mammary Epithelial Cells Induced by Hydrogen Peroxide. Chinese Journal of Animal Nutrition, 2021, 33(1): 350-358.
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