Abstract：This study aimed to investigate the effects of aging corn on performance, digestive and antioxidant functions and egg quality of laying hens. A total of 288 Hy-Line laying hens (60-week of age) were randomly divided into 4 groups with 6 replicates per group and 12 hens per replicate. Aging corn replaced equivalent amount of normal corn used in the basal diet at various levels, and the four dietary treatments were as follows: 1) control group: basal diet (normal corn); 2) 25% aging corn group: aging corn replaced equivalent amount of normal corn in the basal diet at a level of 25%; 3) 50% aging corn group: aging corn replaced equivalent amount of normal corn in the basal diet at a level of 50%; 4) 100% aging corn group: aging corn replaced equivalent amount of normal corn in the basal diet at a level of 100%. The pre-experimental period lasted for 1 week, and the experimental period lasted for 8 weeks. The results showed as follows: 1) compared with the control group, the laying rate of 100% aging corn group was significantly decreased (P<0.05). 2) Compared with the control group, the content of glucose (GLU) and albumin (ALB) in serum of laying hens of 100% aging corn group were significantly decreased (P<0.05), the activity of trypsin in anterior intestinal chyme of laying hens of 50% and 100% aging corn groups was significantly decreased (P<0.05). 3) Compared with the control group, the serum glutathione peroxidase (GSH-Px) activity of laying hens of 25%, 50% and 100% aging corn groups was significantly decreased (P<0.05), and the serum malondialdehyde (MDA) content of laying hens of 100% aging corn group was significantly increased (P<0.05). Compared with the control group, the liver GSH-Px activity of laying hens of 50% and 100% aging corn groups was significantly decreased (P<0.05), the liver catalase (CAT) activity of laying hens of 100% aging corn group was significantly decreased (P<0.05), the liver MDA content of laying hens of 50% and 100% aging corn groups was significantly increased (P<0.05), and the liver protein carbonyl (PC) content of laying hens of 100% aging corn group was significantly increased (P<0.05). 4) Compared with the control group, the egg yolk color of 100% aging corn group on day 56 was significantly decreased (P<0.05). It is concluded that laying rate is decreased with the increasing proportions of aging corn, and 100% aging corn diet could significantly decrease the laying rate, reduce the contents of GLU and ALB in serum, affect the digestive function of anterior intestine, decrease the antioxidant function of serum and liver, and have a negative effect on yolk color.
杨露,赵江涛,谭会泽,等.储藏玉米陈化机制及品质判定进展[J].饲料研究,2016(24):49-53. YANG L,ZHAO J T,TAN H Z,et al.Advances in aging mechanism and quality judgment of stored corn[J].Feed Research,2016(24):49-53.(in Chinese)
[35]
周显青,张玉荣,张勇.储存玉米生理指标的研究[J].玉米科学,2007,15(4):96-99. ZHOU X Q,ZHANG Y R,ZHANG Y.Studies on the physi1ological indexes of stored-maize[J].Journal of Maize Sciences,2007,15(4):96-99.(in Chinese)
[5]
张玉荣,周显青,张勇.储存玉米膜脂过氧化与生理指标的研究[J].中国农业科学,2008,41(10):3410-3414. ZHANG Y R,ZHOU X Q,ZHANG Y,et al.Research on membrane lipid peroxidation and physiological parameters of storage maize[J].Scientia Agricultura Sinica,2008,41(10):3410-3414.(in Chinese)
[15]
YIN F G,LIU Y L,YIN Y L,et al.Dietary supplementation with Astragalus polysaccharide enhances ileal digestibilities and serum concentrations of amino acids in early weaned piglets[J].Amino Acids,2009,37(2):263-270.
[25]
LUO B,CHEN D W,TIAN G,et al.Effects of dietary aged maize with oxidized fish oil on growth performance,antioxidant capacity and intestinal health in weaned piglets[J].Animals,2019,9(9):624.
[4]
周显青,张玉荣,张勇.储藏玉米陈化机理及挥发物与品质变化的关系[J].农业工程学报,2008,24(7):242-246. ZHOU X Q,ZHANG Y R,ZHANG Y,et al.Ageing mechanism of stored maize and the relationship between its volatile components and quality[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(7):242-246.(in Chinese)
[6]
权萌萌,鞠兴荣,石嘉怿.稻谷蛋白质氧化及其对稻谷陈化品质的影响[J].粮食与饲料工业,2015(8):9-13. QUAN M M,JU X R,SHI J Y,et al.Effects of protein oxidation on aging quality of rice[J].Cereal & Feed Industry,2015(8):9-13.(in Chinese)
[10]
李锦英,陈代文,郑萍,等.饲粮添加陈化玉米和复合抗氧化剂对仔猪生长性能、抗氧化功能及肝脏损伤的影响[J].四川农业大学学报,2019,37(1):83-91. LI J Y,CHEN D W,ZHENG P,et al.Effects of aging corn and antioxidant blend supplementation on growth performance,antioxidant capacity and liver injure of weaning piglets[J].Journal of Sichuan Agricultural University,2019,37(1):83-91.(in Chinese)
[14]
张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:49-79. ZHANG L Y.Analysis of feed and feed quality detection technology[M].3rd ed.Beijing:China Agricultural University Press,2007:49-79.(in Chinese)
[16]
ZHANG X L,LU P,XUE W Y,et al.An evaluation of heat on protein oxidation of soy protein isolate or soy protein isolate mixed with soybean oil in vitro and its consequences on redox status of broilers at early age[J].Asian-Australasian Journal of Animal Sciences,2017,30(8):1135-1142.
[20]
朱正鹏,王瑜铭,丁莹,等.陈化玉米对肉鸭生长性能、抗氧化功能及免疫器官指数的影响[J].中国畜牧杂志,2018,54(11):71-74. ZHU Z P,WANG Y M,DING Y,et al.Effects of aged corn on growth performance,serum antioxidant activity and immune organ indexes of meat ducks[J].Chinese Journal of Animal Science,2018,54(11):71-74.(in Chinese)
[24]
刘洋,刘比一,尹达菲,等.不同储存时间的玉米对肉鸡血清生化和组织学的影响[J].中国家禽,2015,37(13):22-26. LIU Y,LIU B Y,YIN D F,et al.Effect of corns at different storage periods on serum biochemical parameters and organ histology of broilers[J].China Poultry,2015,37(13):22-26.(in Chinese)
[26]
李锦英,陈代文,郑萍,等.饲粮添加陈化玉米和复合抗氧化剂对仔猪空肠屏障及后肠微生物和代谢产物的影响[J].中国兽医学报,2019,39(9):1840-1849. LI J Y,CHEN D W,ZHENG P,et al.Effects of aging corn and complex antioxidant supplementation on the jejunal barrier and hindgut microbes and metabolities of piglets[J].Chinese Journal of Veterinary Science,2019,39(9):1840-1849.(in Chinese)
[30]
GRUNE T,JUNG T,MERKER K,et al.Decreased proteolysis caused by protein aggregates,inclusion bodies,plaques,lipofuscin,ceroid,and ‘aggresomes’ during oxidative stress,aging,and disease[J].The International Journal of Biochemistry & Cell Biology,2004,36(12):2519-2530.
[34]
CHEN X X,ZHANG L,LI J L,et al.Hydrogen peroxide-induced change in meat quality of the breast muscle of broilers is mediated by ROS generation,apoptosis,and autophagy in the NF-κB signal pathway[J].Journal of Agricultural and Food Chemistry,2017,65(19):3986-3994.
[1]
胡元森,段永康,王改利,等.不同储藏年限玉米主要品质变化研究[J].农业机械,2011(6):64-67. HU Y S,DUAN Y K,WANG G L,et al.Study on main quality changes of maize in different storage years[J].Farm Machinery,2011(6):64-67.(in Chinese)
[7]
黄友如,华欲飞,裘爱泳.脂质氧化诱导的大豆蛋白质聚集机理的研究[J].中国粮油学报,2006,21(1):80-87. HUANG Y R,HUA Y F,QIU A Y.Mechanism of aggregation of soybean proteins induced by lipid oxidation[J].Journal of the Chinese Cereals and Oils Association,2006(1):80-87.(in Chinese)
[11]
孙得发.2016-2017年中国饲料企业使用陈化玉米导致的黄膘肉问题及思考[J].中国畜牧杂志,2017,53(7):147-151,166. SUN D F.The yellow fat problem caused by the use of aged corn in Chinese feed enterprises from 2016 to 2017 and its thinking[J].Chinese Journal of Animal Science,2017,53(7):147-151,166.(in Chinese)
[17]
LEVINE R L,GARLAND D,OLIVER C N,et al.Determination of carbonyl content in oxidatively modified proteins[J].Methods in Enzymology,1990,186:464-478.
[21]
YIN D F,YUAN J M,GUO Y M,et al.Effect of storage time on the characteristics of corn and efficiency of its utilization in broiler chickens[J].Animal Nutrition,2017,3(3):252-257.
[27]
RIDEAU N,NITZAN Z,MONGIN P.Activities of amylase,trypsin and lipase in the pancreas and small intestine of the laying hen during egg formation[J].British Poultry Science,1983,24(1):1-9.
[31]
YU B P.Cellular defenses against damage from reactive oxygen species[J].Physiological Reviews,1994,74(1):139-162.
[36]
SILVERSIDES F G,SCOTT T A.Effect of storage and layer age on quality of eggs from two lines of hens[J].Poultry Science,2001,80(8):1240-1245.
[37]
沈曼曼,王莹.影响蛋黄颜色沉积因素分析[J].广东饲料,2014,23(6):43-45. SHEN M M,WANG Y.Analysis of factors affecting egg yolk color deposition[J].Guangdong Feed,2014,23(06):43-45.(in Chinese)
[3]
王改利.玉米储藏期霉菌活动、品质变化及挥发性物质成分分析[D].硕士学位论文.郑州:河南工业大学,2010:8-13. WANG G L.Fungal activity,quality changes and analysis of volatile compounds in stored corn[D].Master's Thesis.Zhengzhou:Henan University of Technology,2010:8-13.(in Chinese)
[13]
QAMAR S H.Effect and mechanism of aging corn and rice bran oil on performance,antioxidative status,intestinal physiology and meat quality of meat duck[D].博士学位论文.雅安:四川农业大学,2019:58-92. QAMAR S H.Effect and mechanism of aging corn and rice bran oil on performance,antioxidative status,intestinal physiology and meat quality of meat duck[D].Ph.D.Thesis.Ya'an:Sichuan Agricultural University,2019:58-92.
[23]
BARTOV I.Effect of storage duration on the nutritional value of corn kernels for broiler chicks[J].Poultry Science,1996,75(12):1524-1527.
[33]
DRÖGE W.Free radicals in the physiological control of cell function[J].Physiological Reviews,2002,82(1):47-95.
[8]
郭玉宝.大米储藏陈化中蛋白质对其糊化特性的影响及其相关陈化机制研究[D].博士学位论文.南京:南京农业大学,2012:103-117. GUO Y B.The effects of protein on pasting properties of rice during storage ageing and its related ageing mechanism[D].Ph.D.Thesis.Nanjing:Nanjing Agricultural University,2012:103-117.(in Chinese)
[9]
赵同芳.粮食品质研究概述[J].粮食储藏,1983(6):24-29. ZHAO T F.Overview of food quality research[J].Grain Storage,1983(6):24-29.(in Chinese)
[19]
尹阳阳,卞科,魏红艳.稻谷储藏过程中巯基与质构特性的关系[J].农产品加工,2010(6):71-73. YIN Y Y,BIAN K,WEI H Y.The relationship between sulfhydryl groups and texture characteristics in rice storage[J].Farm Products Processing,2010(6):71-73.(in Chinese)
[29]
ABDELRAHIM M,MORRIS E,CARVER J,et al.Liquid chromatographic assay of dityrosine in human cerebrospinal fluid[J].Journal of Chromatography B:Biomedical Sciences and Applications,1997,696(2):175-182.
[39]
UYGUN-SARIBAY M,ERGUN E,KÖSEOĞLU T.Effect of gamma irradiation and storage on lutein and zeaxanthin in liquid,frozen and dried egg yolk samples[J].Journal of Radioanalytical and Nuclear Chemistry,2014,301(2):597-605.
[12]
LIU J B,YAN H L,ZHANG Y,et al.Effects of stale maize on growth performance,immunity,intestinal morphology and antioxidant capacity in broilers[J].Asian-Australasian Journal of Animal Sciences,2020,33(4):605-614.
[18]
吴大伟,陈星,洑琴,等.常温储存和干热处理对豆粕蛋白质氧化的影响[J].中国粮油学报,2014,29(5):90-94. WU D W,CHEN X,FU Q,et al.Effects of storing and heating on protein oxidation of soybean meal[J].Journal of the Chinese Cereals and Oils Association,2014,29(5):90-94.(in Chinese)
[22]
CABELL C A,ELLIS N R.Feeding value of stored corn[J].Journal of Animal Science,1955,14(4):1167-1173.
[28]
王坤,何青芬,程业飞,等.维生素E缓解蛋白质氧化豆粕对蛋鸡生产性能、消化功能和鸡蛋品质的影响[J].南京农业大学学报,2020,43(2):318-325. WANG K,HE Q F,CHENG Y F,et al.The protective effects of vitamin E on laying performance,digestive function and egg quality in laying hens fed with protein-oxidized soybean meal[J].Journal of Nanjing Agricultural University,2020,43(2):318-325.(in Chinese)
[32]
MICHIELS C,RAES M,TOUSSAINT O,et al.Importance of Se-glutathione peroxidase,catalase,and Cu/Zn-SOD for cell survival against oxidative stress[J].Free Radical Biology and Medicine,1994,17(3):235-248.
[38]
BLOUNT J D,HOUSTON D C,MOLLER A P.Why egg yolk is yellow[J].Trends in Ecology & Evolution,2000,15(2):47-49.
[40]
VAN HET HOF K H,WEST C E,WESTSTRATE J A,et al.Dietary factors that affect the bioavailability of carotenoids[J].The Journal of Nutrition,2000,130(3):503-506.
