Effects of Straw and Alfalfa Hay Treated by Enzyme and Bacteria on Growth Performance, Serum Biochemical Indexes, Rumen Bacterial Diversity and KEGG Pathway of Tan Sheep
JIANG Biwei1,2, ZHOU Yuxiang1, WANG Tian1, LI Fei1
1. College of Agriculture, Ningxia University, Yinchuan 750021, China;
2. Ningxia Vocational and Technical College, Yinchuan 750021, China
Abstract：This experiment was conducted to study the effects of straw and alfalfa hay treated by enzyme and bacteria on growth performance, serum biochemical indexes, rumen bacterial diversity and KEGG pathway of Tan sheep. Twenty 3-month-old NingxiaTan sheep with similar weight and good health condition were randomly divided into 2 groups with 10 sheep in each group. The ratio of concentrate to roughage was 30:70, and the roughage was consisted of straw and alfalfa hay with the ratio of straw to alfalfa was 60:40. The straw and alfalfa hay in the control group was not treated, but those in the trial group was treated by compound probiotics (main components were yeast, Bacillus subtilis and lactic acid bacteria) and cellulase (activity≥10 000 U/g). The pre feeding period was 15 days and the normal feeding period was 60 days. Blood samples were collected from abdominal vein on days 1, 30 and 60 of the trial period for determination of serum biochemical indexes. At the end of feeding experiment, rumen fluid was collected on an empty stomach and DNA was extracted for rumen bacterial diversity and metagenome analysis. The results showed as follows: 1) the total gain and average daily gain of Tan sheep in the trial group were significantly higher than those in the control group (P<0.01), and the feed/gain was significantly lower than that in the control group (P<0.01). 2) The contents of serum total protein and globulin in the trial group were significantly higher than those in the control group (P<0.05), and the serum urea content was significantly lower than that in the control group (P<0.01). 3) The number of operational taxonomic unit (OTU) and Shannon index in the trial group were significantly higher than those in the control group (P<0.01); there were 8 different bacteria phyla between the trial group and the control group, they were Proteobacteria, Patescibacteria, Bacteriodetes, Kiritimatiellaeota, Planctomycetes, Cyanobactria, Spirochaetes and Synergistetes. 4) In KEGG pathway, the number of genes in pyrimidine metabolism, amino sugar and nucleotide sugar metabolism and glyoxylate and dicarboxylate metabolism in the trial group was significantly higher than that in the control group (P<0.01). It is concluded that, the enzyme and bacteria mixed treatment of straw and alfalfa hay can improve the weight gain and the economic benefits of Tan sheep, at the same time, the treatment method improves the serum total protein and globulin contents, decreases the serum urea content, the changes the diversity of rumen bacteria and the number of some functional genes of Tan sheep. Under this experiment condition, the feeding effect of Tan sheep with straw and alfalfa hay after enzyme and bacteria mixed treatment is better, which is beneficial to the healthy breeding of Tan sheep and can be popularized in production.
姜碧薇, 周玉香, 王甜, 李斐. 酶菌混合处理稻草和苜蓿干草对滩羊生长性能、血清生化指标、瘤胃细菌多样性及KEGG通路的影响[J]. 动物营养学报, 2021, 33(3): 1482-1492.
JIANG Biwei, ZHOU Yuxiang, WANG Tian, LI Fei. Effects of Straw and Alfalfa Hay Treated by Enzyme and Bacteria on Growth Performance, Serum Biochemical Indexes, Rumen Bacterial Diversity and KEGG Pathway of Tan Sheep. Chinese Journal of Animal Nutrition, 2021, 33(3): 1482-1492.
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