Abstract：This experiment was conducted to investigate the effects of dietary guanidineacetic acid (GAA) on growth performance, slaughter performance and meat quality of lambs. Forty-eight 4-months-old Dorper×thin-tailed Han F1 hybrid ram lambs with similar body weight of (24.8±1.3) kg were randomly divided into 4 groups with 12 sheep in each group. Lambs in four groups were fed basal diets supplemented with 0 (control group), 300 (300GAA group), 600 (600GAA group) and 900 mg/kg (900GAA group) GAA, respectively. The first 15 days were for adaption, and the experimental period lasted for 70 days. The results showed as follows: 1) the final body weight, average daily gain of lambs of 900GAA group were significantly higher than those of control group, 300GAA group and 600GAA group (P<0.05), and the feed to gain ratio of lambs of 900GAA group was significantly lower than that of control group, 300GAA group and 600GAA group (P<0.05). 2) The carcass weight and net meat weight of 900GAA group were significantly higher than those of the control group (P<0.05), and the net meat percentage and eye muscle area of 300GAA group, 600GAA group and 900GAA group were significantly higher than those of the control group (P<0.05). There were no significant differences in organ indexes among all groups (P>0.05). 3) The ileum length of 300GAA group, 600GAA group and 900GAA group was significantly higher than that of the control group (P<0.05), and the ileum weight of 900GAA group was significantly higher than of control group, 300GAA group, 600GAA group (P<0.05). 4) The longissimus dorsi pH after slaughter 24 h of 300GAA group, 600GAA group and 900GAA group was significantly higher than that of the control group (P<0.05), the longissimus dorsi brightness (L*) value after slaughter 24 h of 600GAA group was significantly higher than that of control group and 300GAA group (P<0.05), the longissimus dorsi water holding capacity of 300GAA group, 600GAA group and 900GAA group was significantly higher than that of the control group (P<0.05), and the longissimus dorsi crude protein content of 600GAA group and 900GAA group was significantly higher than that of the control group (P<0.05). In conclusion, dietary appropriate supplementation of GAA can improve the growth performance, slaughter performance of lambs, and improve the meat quality. In this experiment, the dietary optimal GAA supplemental level is 900 mg/kg.
张俊玲,张德福,石凤云,等.胍基乙酸在动物生产上的研究进展[J].中国畜牧杂志,2016,52(4):63-66. ZHANG J Z,ZHANG D F,SHI F Y,et al.Research process for guanidino acetic acid in animal production[J].Chinese Journal of Animal Science,2016,52(4):63-66.(in Chinese)
[13]
朱宇旌,郑兰宇,张勇,等.饲料中钙含量测定方法的比较[J].畜牧与兽医,2009,41(9):51-53. ZHU Y J,ZHENG L Y, ZHANG Y,et al.Comparison of determination methods of calcium content in feeds[J].Animal Husbandry & Veterinary Medicine,2009,41(9):51-53.(in Chinese)
[11]
AOAC.Official methods of analysis of AOAC international[S].19th ed.Gaithersburg:AOAC International,2012.
[34]
周光宏.肉品学[M].北京:中国农业科技出版社,1999. ZHOU G H.Meat science[M].Beijing:Agriculture and Science Press,1999.(in Chinese)
[1]
张德福,田耀耀,马佳,等.胍基乙酸对AA肉仔鸡生长性能和经济效益的影响[J].饲料研究,2016(17):32-35. ZHANG D F,TIAN Y Y,MA J,et al.Effects of guanidine acetic acid on growth performance and economic benefit of AA broilers[J].Feed Research,2016(17):32-35.(in Chinese)
[5]
LEMME A,RINGEL J,STERK A,et al.Supplemental guanidino acetic acid affects energy metabolism of broilers[C]//Proceedings of the 16th European symposium on poultry nutrition.Strasbourg France:[s.n.],2007.
[7]
WANG L S,SHI B M,SHAN A S,et al.Effects of guanidinoacetic acid on growth performance,meat quality and antioxidation in growing-finishing pigs[J].Journal of Animal and Veterinary Advances,2012,11(5):631-636.
[9]
晁雅琳,刘博,寇启芳,等.胍基乙酸对舍饲滩羊生长性能、屠宰性能、脂肪沉积及肌肉营养成分的影响[J].动物营养学报,2019,31(1):388-394. CHAO Y L,LIU B,KOU Q F,et al.Effects of guanidine acetic acid on growth performance,slaughter performance,fat deposition and nutritional components in muscle of stabling Tan Sheep[J].Chinese Journal of Animal Nutrition,2019,31(1):388-394.(in Chinese)
[15]
MICHIELS J,MAERTENS L,BUYSE J,et al.Supplementation of guanidinoacetic acid to broiler diets:effects on performance,carcass characteristics,meat quality,and energy metabolism[J].Journal of Poultry Science,2012,91(2):402-412.
[17]
EMAMI N K,GOLIAN A,RHOADS D D,et al.Interactive effects of temperature and dietary supplementation of arginine or guanidinoacetic acid on nutritional and physiological responses in male broiler chickens[J].British Poultry Science,2017,58(1):87-94.
[6]
MOUSAVI S N,AFSAR A,LOTFOLLAHIAN H.Effects of guanidinoacetic acid supplementation to broiler diets with varying energy contents[J].Journal of Applied Poultry Research,2013,22(1):47-54.
[10]
刘洁.肉用绵羊饲料代谢能与代谢蛋白质预测模型的研究[D].博士学位论文.北京:中国农业科学院,2012. LIU J.Prediction of metabolizable energy and metabolizable protein in feeds for meat sheep[D].Ph.D.Thesis.Beijing:Chinese Academy of Agricultural Sciences,2012.(in Chinese)
[16]
潘宝海,孙冬岩,田耀耀.胍基乙酸对育肥猪生长性能、胴体品质及肉品质的影响[J].中国畜牧杂志,2016,52(19):38-41. PAN B H,SUN D Y, TIAN Y Y.Effects of guanidinoacetic acid on growth performance,carcass traits and meat quality in finishing pigs[J].Chinese Journal of Animal Science,2016,52(19):38-41.(in Chinese)
[20]
肖玉琪,张有法,杨若飞,等.绵羊眼肌面积近似计算公式法初探[J].中国草食动物,2003(增刊1):117-118. XIAO Y Q,ZHANG Y F,YANG R F,et al.A preliminary study on the approximate formula of sheep eye muscle area[J].China Herbivores,2003(Suppl.1):117-118.(in Chinese)
[26]
谷英,孙海洲,桑丹,等.肉品质评定指标及影响因素的研究进展[J].中国畜牧兽医,2013,40(7):100-106. GU Y,SUN H Z,SANG D,et al.Research progress on evaluation indices and influencing factors of meat quality[J].China Animal Husbandry & Veterinary Medicine,2013,40(7):100-106.(in Chinese)
[30]
李光全,邓位喜,李佳剑,等.务川黑牛不同杂交组合屠宰性能及肉质研究[J].中国畜牧杂志,2019,55(7):63-66. LI G Q,DENG W X,LI J J,et al.Study on meat yield and meat quality of different hybrid combinations Wuchuan black cattle[J].Chinese Journal of Animal Science,2019,55(7):63-66.(in Chinese)
[2]
王誉杰,张进威,王讯,等.胍基乙酸及代谢产物肌酸的研究进展[J].畜牧兽医学报,2018,49(8):1577-1584. WANG Y J,ZHANG J W,WANG X,et al.The research progress of guanidinoacetic acid and creatine[J].Acta Veterinaria et Zootechnica Sinica,2018,49(8):1577-1584.(in Chinese)
[4]
SCHEFFLER T L,KASTEN S C,ENGLAND E M,et al.Contribution of the phosphagen system to postmortem muscle metabolism in AMP-activated protein kinase γ3 R200Q pig longissimus muscle[J].Meat Science,2014,96(2):876-883.
[14]
李会娟.2种植物磷含量的检测方法比较研究[J].现代农业科技,2012(11):16-17. LI H J.Comparative study on determination of phosphorus content in two kinds of plants[J].Modern Agricultural Sciences,2012,(11):16-17.(in Chinese)
[24]
常银莲.支链氨基酸对肉鸡小肠发育及TOR通路相关基因表达的影响[D].硕士学位论文.北京:中国农业科学院,2016. CHANG Y L.Effects of BCAAs on small intestinal development and relative genes expression of TOR pathway in broilers[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2016.(in Chinese)
[8]
洑琴,乔丽红,唐志刚,等.胍基乙酸对建鲤生产性能、体成分及肌肉能量代谢关键酶的影响[J].中国粮油学报,2015,30(3):85-89. FU Q,QIAO L H,TANG Z G,et al.Effects of guanidinoacetic acid on growth performance,body composition and key enzymes of energy metabolism of muscle in Jian Carp[J].Journal of The Chinese Cereals and Oils Association,2015,30(3):85-89.(in Chinese)
[12]
VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.
[22]
赵艳兵,任有蛇,张春香,等.日粮中添加荞麦秸秆对绵羊屠宰性能、器官指数、肉品质及血液生化指标的影响[J].山西农业大学学报(自然科学版),2017,37(12):872-878. ZHAO Y B,REN Y S,ZHANG C X,et al.Effects of adding buckwheat straw to the diet on slaughter performance,organ index,meat quality and blood biochemical indexes of sheep[J].Journal of Shanxi Agricultural University (Nature Science Edition),2017,37(12):872-878.(in Chinese)
[32]
张美琦,李妍,李树静,等.饲粮能量水平对13-18月龄荷斯坦阉牛生产性能和屠宰指标的影响[J].畜牧兽医学报,2020,51(6):1295-1305. ZHANG M Q,LI Y,LI S J,et al.Effects of dietary energy level on production performance and slaughter indicators of 13 to 18 months old Holstein Steers[J].Chinese Journal of Animal and Veterinary Science,2020,51(6):1295-1305.(in Chinese)
[18]
万凡,马涛,马晨,等.不同饲养标准对杜寒杂交肉用绵羊生产和屠宰性能的影响[J].动物营养学报,2016,28(11):3483-3492. WAN F,MA T,MA C,et al.Effects of different feeding standards on growth and slaughter performance of Dorper×Thin-Tailed Han crossbred meat lambs[J].Chinese Journal of Animal Nutrition,2016,28(11):3483-3492.(in Chinese)
[19]
常庆玲,崔亚利,敦伟涛,等.寒泊羊与小尾寒羊育肥效果及肉品质比较研究[J].中国畜牧杂志,2014,50(5):75-79. CHANG Q L,CUI Y L,DUN W T,et al.Difference of fattening effect and meat quality between Hanper sheep and small-tail sheep[J].Chinese Journal of Animal Science,2014,50(5):75-79.(in Chinese)
[21]
江涛.胍基乙酸的合成及其对肉鸡生长性能和血液理化指标的影响[D].硕士学位论文.合肥:安徽农业大学,2012. JIANG T.The synthesis of guanidine acetic acid and the influence of broiler growth performance and blood biochemical indexes[D].Master's Thesis.Hefei:Anhui Agricultural University,2012.(in Chinese)
[23]
官丽辉,刘海斌,张立永,等.日粮不同能量水平对育成鸡体增质量、血液生化指标及内脏器官发育的影响[J].中国兽医学报,2014,34(2):350-356. GUAN L H,LIU H B,ZHANG L Y,et al.Effect of different metabolic energy in diet on daily weight gain,serum[J].Chinese Journal of Veterinary Science,2014,34(2):350-356.(in Chinese)
[25]
周世霞.日粮粗纤维水平对朗德鹅生长性能、血清生化指标和胃肠道发育的影响[D].硕士学位论文.武汉:华中农业大学,2006. ZHOU S X.Effect of crude fiber level diets on growth performance,serum biochemical parameters and gastrointestinal development in Landes geese[D].Master's Thesis.Wuhan:Huazhong Agricultural University,2006.(in Chinese)
[27]
杨文军,牟春堂,王鹏举,等.饲粮中添加葡萄籽原花青素对羔羊生长性能、屠宰性能、肉品质及血清抗氧化指标的影响[J].动物营养学报,2020,32(6):2755-2764. YANG W J,MU C T,WANG P J,et al.Effects of dietary supplementation of grape seed proanthocyanidins on growth performance,slaughter performance,meat quality and serum antioxidant indexes of lambs[J].Chinese Journal of Animal Nutrition,2020,32(6):2755-2764.(in Chinese)
[28]
刘洋,李蛟龙,张林,等.胍基乙酸和甜菜碱对育肥猪肌肉能量代谢和肉品质的影响[J].畜牧兽医学报,2015,46(9):1557-1563. LIU Y,LI J L,ZHANG L,et al.Effects of dietary supplementation of guanidinoacetic acid and combination of guanidinoacetic acid and betaine on muscle energy metabolism,meat quality in finishing pigs[J].Chinese Journal of Animal and Veterinary Science,2015,46(9):1557-1563.(in Chinese)
[29]
刘瑞生,王珂,徐建峰,等.中草药添加剂改善羊肉品质的研究进展[J].中国畜牧杂志,2020,56(7):42-46. LIU R S,WANG K,XU J F,et al.Research progress on Chinese herbal medicine additives improving mutton quality[J].Chinese Journal of Animal Science,2020,56(7):42-46.(in Chinese)
[31]
曹忻,张丽,张文涛,等.不同尾型绵羊生产性能、屠宰性能、肉品质和脂肪酸组成的比较[J].西北农业学报,2020,29(1):1-10. CAO X,ZHANG L,ZHANG W T,et al.Growth and slaughter performance,meat quality,and fatty acid of sheep with distinct tail types[J].Acta Agriculturae Boreali-Occidentalis Sinica,2020,29(1):1-10.(in Chinese)
[33]
汪骁轩,高静雯,魏殿华,等.强冷应激对阿勒泰及杂交种羔羊脂质代谢相关基因mRNA表达量及脂肪沉积的影响[J].中国畜牧杂志,2020,56(3):51-56. WANG X X,GAO J W,WEI D H,et al.Effects of strong cold stress on mRNA expression of lipid metabolism related genes and fat changes in Altay and hybrid sheep[J].Chinese Journal of Animal Science,2020,56(3):51-56.(in Chinese)
[35]
赵元,许迟,吴仙花,等.不同浓度水平胍基乙酸对育肥猪肉品质影响的研究[J].中国饲料,2018(17):27-31. ZHAO Y,XU C,WU X H,et al.Study on effects of dietary supplementation guanidinoacetic acid (GAA) on fattening pork quality[J].China Feed,2018(17):27-31.(in Chinese)
