本试验旨在研究环境温度和维生素A(VA)对育成期(12~17周龄)笼养公鸭睾丸发育及相关激素的影响。选取12周龄金定公鸭144只,采用2×3两因素交互试验设计,随机分配到适宜舍温[(18±1) ℃]和较低舍温[(2±1) ℃]下的3个VA水平组(4 000、10 000和15 000 IU/kg)中,每组6个重复,每个重复4只试鸭。结果表明:(1)环境温度对三碘甲腺原氨酸(T3)和甲状腺素(T4)影响极显著(P<0.01),对胰岛素影响不显著(P>0.05);VA水平对T3影响不显著(P>0.05),对T4影响极显著(P<0.01),对胰岛素影响不显著(P>0.05);环境温度和VA水平对胰岛素有交互作用(P<0.05);(2)环境温度对血清促卵泡生成激素(FSH)影响显著(P<0.05),对血清促黄体生成激素(LH)和血清睾酮(T)影响极显著(P<0.01),VA水平对FSH和T影响不显著(P>0.05),对LH影响显著(P<0.05);(3)环境温度对睾丸相对重量和睾丸相对长度影响显著(P<0.05);VA水平对睾丸相对重量和相对长度影响均不显著(P>0.05)。本试验条件下,15 000 IU/kg的VA能有效地调节较低舍温对激素水平产生的影响,使其与正常生理水平相接近,10 000 IU/kg的VA能降低较低舍温对睾丸发育造成的不利影响。
This experiment was conducted to study the effects of environmental temperature and vitamin A on testicular development and relative hormones of rearing drake (12~17 weeks) in cage. A 2×3 factorial arrangement of treatment was employed with 2 temperature levels (suitable indoor temperature (18±1) ℃ and lower indoor temperature (2±1) ℃) and 3 vitamin A levels (4 000, 10 000 and 15 000 IU/kg) .One hundred and forty-four drakes were randomly allotted into 6 groups and each group contained 6 replicates with 4 drakes. The results showed as follows: 1) environmental temperature significantly affected triiodothyronine (T3) and thyroxine (T4) (P<0.01) but had not significant influenceon insulin (P>0.05). The T4 level was significantly affected by vitamin A levels (P<0.01); however, T3 and insulin were not significantly affected by vitamin A levels (P>0.05). There was significant effect of interaction of environmental temperature and vitamin A levels on insulin level (P<0.05); 2) follicle stimulating hormone (FSH) (P<0.05), luteinzing hormone (LH) (P<0.01) and testosterone (T) in serum (P<0.01) were significantly influenced by environmental temperature. Vitamin A levels significantly affected LH level (P<0.05), but had not significantly influenced on the level of FSH and T (P>0.05); 3) testicular relative weight and testicular relative length were significantly influenced by environmental temperature (P<0.05), however, vitamin A levels did not influence testicular relative weight and testicular relative length (P>0.05). In conclusion, it would appear from the results of the present study that 15 000 IU/kg vitamin A could efficiently regulate the thyroid hormone levels which were influenced by lower indoor temperature, and make them closed to normal physiology standard; and the 10 000 IU/kg vitamin A could reduce the adverse effects of lower indoor temperature on testicular development of drakes.[Chinese Journal of Animal Nutrition, 2009,21(6):829-833]