本试验旨在通过对血清总超氧化物歧化酶(T-SOD)活性、过氧化氢酶(CAT)活力和丙二醛(MDA)含量的测定，评价不饱和脂肪酸不同添加比例对黄鳝脂质过氧化状态的影响。试验日粮按L9(33)正交设计在不含不饱和脂肪酸的基础日粮中添加红花籽油(C18∶2n-6来源)、亚麻籽油(C18∶3n-3来源)和EPA/DHA混合油脂(C20∶5n-3+C22∶6n-3来源)。选用平均体长(34.5±2.4) cm、平均体重(37.8±3.5) g的黄鳝1 000尾，随机分为对照组和9个试验组，每组2个重复，每个重复50尾。对照组投喂基础日粮，试验组分别投喂1种试验日粮，试验期31 d。结果显示：与对照组相比，各试验组血清中SOD活性差异不显著(P>0.05)。各试验组血清中CAT活力均有不同程度降低，但与对照组差异不显著(P>0.05)。各试验组血清MDA含量均有不同程度降低，且1组、3组、4组、5组、6组、7组和8组显著低于对照组(P<0.05)。综合生长试验可知，在保持较高生长性能的同时，当日粮中不饱和脂肪酸C18∶2n-6、C18∶3n-3和C20∶5n-3+C22∶6n-3含量分别为1.30%、1.55%和0.25%时，黄鳝体内自由基的代谢及脂质过氧化处于最佳平衡状态。

The total superoxide dismutase (T-SOD), catalase (CAT) and malondialdehyde (MDA) in serum were measured to evaluate the effect of different supplemental levels of unsaturated fatty acid on lipid peroxidation in Monopterus albus. Through the L9(33) orthogonal design, the experimental diets were added with different levels of safflower seed oil (source of C18∶2n-6), linseed oil (source of C18∶3n-3) and EPA/DHA mixture oil (source of C20∶5n-3+C22∶6n-3) on a basal diet. One thousand Monopterus albus with average body length of (34.5±2.4) cm and average body weight of (37.8±3.5) g were randomly divided into control group and 9 experimental groups, and each group contained 2 replicates and each replicate contained 50 Monopterus albus. Control group fed with basal diet, and each experimental group fed with 1 experimental diet. The experiment lasted for 31 days. The results were showed that, no significant difference was found in serum T-SOD activity among all groups (P>0.05). The serum CAT capacity in experimental groups was lower than that in control group, but no significant difference was found (P>0.05). The serum MDA content in groups 1, 3, 4, 5, 6, 7 and 8 was significant lower than that in control group (P<0.05). Combination with the growth experiment, the metabolism of free radicals and lipid peroxidation kept higher growth performance and the best state of equilibrium when the content of C18∶2n-6, C18∶3n-3 and C20∶5n-3+C22∶6n-3 were 1.30%, 1.55% and 0.25% in diet, respectively.［Chinese Journal of Animal Nutrition, 2009，21(6):1018-1022］