本试验旨在研究谷氨酰胺（Gln）对新生仔猪生长性能、血清激素水平和小肠黏膜中相关酶活性的影响。试验选用4日龄新生仔猪36头，分成3组，每组4个重复，每个重复3头（2公1母），各组日粮中分别添加0（对照组）、0.5%和1.0%谷氨酰胺。试验期18 d。结果表明：与对照组相比，日粮中添加1.0%谷氨酰胺显著提高了仔猪4～14日龄平均日采食量（P<0.05）；日粮中添加0.5%谷氨酰胺显著提高了仔猪14日龄血清胃泌素含量（P<0.05）；添加1.0%谷氨酰胺也显著提高了仔猪21日龄血清胃泌素含量（P<0.05）;仔猪21日龄时，添加0.5%和1.0%谷氨酰胺显著提高了空肠黏膜中鸟氨酸脱羧酶（ODC）、Ca2+,Mg2+-ATP酶和Na+,K+-ATP酶的活性（P<0.05）；添加1.0%谷氨酰胺显著降低了空肠黏膜中谷氨酰胺合成酶（GS）和鸟氨酸转氨酶（OAT）的活性（P<0.05）。日粮中添加1.0%谷氨酰胺显著提高了仔猪14日龄空肠黏膜蔗糖酶、麦芽糖酶和乳糖酶活性（P<0.05）。综上所述，日粮中添加1.0%谷氨酰胺提高了人工饲养新生仔猪14日龄肠道消化能力，改善了21日龄空肠黏膜中与谷氨酰胺代谢相关的酶活性。

This study was conducted to determine the effects of glutamine (Gln) supplementation on growth performance, serum hormone levels and small intestinal mucosal enzyme activities of neonatal piglets. Thirty-six 4-day-old neonatal piglets were divided into 3 groups containing 4 replicates of 3 piglets (2 males and 1 female). Piglets in 3 groups were fed basal diet with supplementation of 0 (control group), 0.5% and 1.0% Gln, respectively. The experiment lasted for 18 d. The results showed as follows: compared with control group, dietary supplementation with 1.0% Gln increased average daily feed intake of piglets at 4～14 d of age (P<0.05). Diet supplemented with 0.5% Gln significantly increased the concentrations of serum gastrin of piglets at 14 d of age (P<0.05); supplementation with 1.0% Gln also significantly increased the concentrations of gastrin in serum at 21 d of age in piglets (P<0.05). At 21 d of age, diet supplemented with 0.5% and 1.0% Gln significantly increased the activities of ornithine decarboxylase, Ca2+,Mg2+-ATPase and Na+,K+-ATPase in jejunal mucosal (P<0.05); supplementation with 1.0% Gln significantly decreased the activities of glutamine synthetase and ornithine aminotransferase (P<0.05). Supplementation with 1.0% Gln significantly increased the activities of sucrase, maltase and lactase in jejunal mucosal of piglets at 14 d of age (P<0.05). In conclusion, dietary supplementation with 1.0% Gln could increase intestinal ability to digest in artificial reared neonatal piglets at 14 d of age, and improve metabolism related enzyme activities with Gln in jejunal mucosal at 21 d of age.［Chinese Journal of Animal Nutrition, 2010，22（1）:125-131］