The aim of the study was to investigate the effects of dietary energy level on expression of lipid anabolism related genes in adipose tissue of Wujin pigs. Fifty-four Wujin pigs with the body weight of 15 kg were randomly assigned into 3 groups with 3 replicates per group and 6 heads in each replicate. Pigs in low digestive energy group (group LDE), middle digestive energy group (group MDE) and high digestive energy group (group HDE) were fed diets with three digestive energy levels, which were 11.74, 12.89 and 14.22 MJ/kg, respectively. At the body weight of 30, 60 and 100 kg, pigs were slaughtered to determine the fat percentage. The subcutaneous adipose tissue was collected for the analysis of expression levels of lipid anabolism related genes [acetyl-CoA carboxylase (ACC), fatty acid synthetase （FAS） and sterol regulatory element-binding protein 1c （SREBP-1c）] and fatty acid transport factor [adipocyte fatty acid-binding protein (A-FABP)] by real-time PCR. The results showed as follows: 1) fat percentage of Wujin pigs was significantly increased with the increasing of dietary digestive energy level at the body weight of 60 and 100 kg (P<0.01); 2) compared with those in group MDE, the expression levels of ACC, FAS and SREBP-1c of pigs in group HDE were significantly decreased at the body weight of 30 and 100 kg （P<0.05）, but significantly increased at the body weight of 60 kg （P<0.05）; 3) the expression level of A-FABP was increased significantly with the increasing of dietary digestive energy level at the body weight of 30 and 100 kg (P<0.05), but decreased significantly at the body weight of 60 kg (P<0.05). The results indicate that high dietary energy level can significantly promote the expression of lipid anabolism related genes (ACC, FAS and SREBP-1c) but significantly inhibit the expression of fatty acid transport factor (A-FABP) and the transportation of free fatty acids. As a result, the de nove synthesis of free fatty acids is increased and the intake of exogenous fatty acids is decreased in the adipose tissue.［Chinese Journal of Animal Nutrition, 2011, 23（5）：781 -788］
［1］王忠庆,荣耀先.大河乌猪与大河猪（乌金猪）肉质形状研究[J].云南农业科技,2004,增刊：41-44.
[2]LIU C Y, GRANT A L, KIM K H, et al. Porcine somatotropin decreases acetyl-CoA carboxylase gene expression in porcine adipose tissue[J]. Domestic Animal Endocrinology, 1994, 11(1):125-132.
[3]JEUKENDRUP A E. Regulation of fat metabolism in skeletal muscle[J]. Annals of the New York Academy of Sciences, 2002, 967:217-35.
[4]SMITH S, WITKOWSKI A, JOSHI A K. Structural and functional organization of the animal fatty acid synthase[J]. Progress in Lipid Research, 2003, 42(4):289-317.
[5]周顺伍.动物生物化学[M].3版.北京:中国农业出版社,1999.
[6]FISCHER P P, WABITSCH M, HOCHBERG Z. Endocrinology of adipose tissue-an update[J]. Hormone and Metabolic Research, 2007, 39(5):314-321.
[7]ASSAF S, HAZARD D, PITEL F, et al. Cloning of cDNA encoding the nuclear form of chicken sterol response element binding protein-2 (SREBP-2), chromosomal localization, and tissue expression of chicken SREBP-1C and -2 genes[J]. Poultry Science, 2003, 82:54-61.
[8]KIM J B, PASHA S, MARGART W, et al. Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1[J] Journal of Clinical Investigation, 1998, 101:1-9.
[9]GERBENS F, JANSEN A, VAN ERP ANTON J M, et al. The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs[J]. Mammalian Genome, 1998b(9):1022-1026.
[10]GERBENS F, VAN ERP ANTON J M, HARDERS F L, et al. Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs[J]. Journal of Animal Science, 1999, 77:846-852.
[11]ZHANG X, ZHAO S M, GE C R, et al. Effects of dietary digestible energy levels on meat quality in Wujin pig[J]. Chinese Journal of Animal Nutrition, 2008, 20(4):377-387.
[12]ZHANG X, ZHAO S M, GE C R, et al. Effects of dietary energy levels on growth performance and carcass composition of Wujin pigs[J]. Chinese Journal of Animal Nutrition, 2008,20(5):489-450.
[13]ZHAO S M, WANG J, SONG X L, et al. Impact of dietary protein on lipid metabolism related gene expression in porcine adipose tissue[J]. Nutrition & Metabolism, 2010(7):6-18.
[14]张宏福,张子仪.动物营养参数与饲养标准:中国肉脂型生长肥育猪饲养标准[M].北京:中国农业出版社,1998.
[15]BIKKER P, VERSTEGEN M W, CAMPBELL R G. Performance and body composition of finishing gilts (45 to 85) as affected by energy intake and nutrition in carlier life[J]. Journal of Animal Science, 1996, 74:817-826.
[16]QUINIOU N, DOURMAD J Y, NOBLET J. Effect of energy intake on the performance of different types of pig from 45 to 100 kg body weight[J]. Animal Science, 1996, 63:277-288.
[17]QUINIOU N, NOBLET J. The Effect of energy supply on the contribution of lean tissue to total body protein mass in pigs slaughtered at 100 kg[J]. Animal Science, 1997, 65:509-513.
[18]刘作华,杨云飞,孔路军,等.日粮能量水平对生长育肥猪肌内脂肪含量以及脂肪酸合成酶和激素敏感脂酶mRNA表达的影响[J].畜牧兽医学报,2007,38(9):934-941
[19]魏彩虹.猪脂肪沉积调控酶的研究进展[J].甘肃畜牧兽医,1997,27(4):29-32.
[20]KATSURADA A, IRIANI N, FUKUDA H, et al. Effects of nutrients and hormones on transcriptional and post-transcriptional regulation of acetyl-CoA carboxylase in rat liver[J]. European Journal of Biochemistry, 1990, 190(2):435-441.
[21]IRITANI N, FUKUDA E, INOGUCHI K. A possible role of Z protein in dietary control of hepatic triacylglycerol synthesis[J]. Journal of Nutritional Science and Vitaminology, 1980, 26:271-277.
[22]MILDNER A M, CLARKE S D. Porcine fatty acid synthase: cloning of a complementary DNA, tissue distribution of its mRNA and suppression of expression by somatotropin and dietary protein[J]. Journal of Nutrition, 1991, 121:900-907.
[23]DING S T, SCHINCKELl A P, WEBER T E, et al. Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations[J]. Journal of Animal Science, 2000, 78:2127-2134.
[24]熊文中,杨凤,周安国.猪重组生长激素对不同杂交肥育猪脂肪代谢调控的研究[J].畜牧兽医学报,2001,32(1):1-4.
[25]BORGSTROM B，BROCKMAN H L. Adipose tissue lipases[C]//BELFRAGE P, FREDRIKSON G, STRALFORS P, et al. Lipases. Amsterdam: Elsevier, 1984： 365-416.
[26]SEMENKOVICH C F. Regulation of fatty acid synthase (FAS)[J]. Progress in Lipid Research, 1997, 36:43-53.
[27]STOECKMAN A K, TOWLE H C. The role of SREBP-1c in nutritional regulation of lipogenic enzyme gene expression[J]. The Journal of Biological Chemistry, 2002, 277:27029-27035.
[28]BROWN M S, Goldstein J L. A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood[J]. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96:11041-11048.
[29]GERBENS F, KONING D J, HARDERS F L, et al. The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs[J]. Journal of Animal Science, 2000, 78:552-559.
[30]GARDAN D, LOUVEAV I, GONDRET F, et al. Adipocyte- and heart-type fatty acid binding proteins are both expressed in subcutaneous and intramuscular porcine (Sus scrofa) adipocytes[J]. Comparative Biochemistry and Physiology, 2007, 148:14-19.
[31]YU Y H, GINSBERG H N. The role of acyl-CoA: diacylglycerol cyltransferase (DGAT) in energy metabolism[J]. Annals of Medicine, 2004, 36(4):252-261.
京公网安备 11010802041926号