本试验旨在通过研究饲喂氧化鱼油对新生仔猪生长性能、肠道黏膜氧化相关指标、免疫因子、氧化和免疫共同相关因子的影响以及添加大豆异黄酮（SI）对其的干预作用，探讨氧化应激对新生仔猪生长、肠道免疫应答的影响机制。试验选用4日龄新生仔猪96头，分成4组，每组6个重复，每个重复4头仔猪，各组饲粮中分别添加5%新鲜鱼油（Ⅰ组）、5%新鲜鱼油+50 mg/kg SI（Ⅱ组）、5%氧化鱼油（Ⅲ组）和5%氧化鱼油+50 mg/kg SI（Ⅳ组）。试验期21 d。试验结束后，每重复选取2头仔猪进行屠宰取样。结果表明：试验第21天Ⅲ组仔猪体重显著低于Ⅱ组（P<0.05）；1～21天Ⅲ组仔猪平均日增重显著低于Ⅰ组和Ⅱ组（P<0.05）。Ⅲ组仔猪肠道黏膜丙二醛（MDA）含量比Ⅰ组和Ⅱ组分别提高了90.89%（P<0.05）和73.28%（P<0.05）。SI添加显著提高肠道黏膜总超氧化物歧化酶（T-SOD）活性（P<0.05）。氧化鱼油和SI对肠道黏膜总抗氧化能力（T-AOC）产生极显著互作效应（P<0.01）。Ⅲ组新生仔猪肠道黏膜白细胞介素-2（IL-2）含量极显著高于Ⅰ组（P<0.01），白细胞介素-10（IL-10）含量极显著低于Ⅰ组(P<0.01)，白细胞介素-8（IL-8）含量显著低于Ⅰ组（P<0.05）。氧化鱼油显著提高了新生仔猪肠道黏膜诱导型一氧化氮合成酶（iNOS）活性（P<0.05），极显著提高了核因子-κB（NF-κB）含量（P<0.01）。综上所述，饲粮中添加氧化鱼油显著降低了新生仔猪（4～24日龄）肠道抗氧化能力，引发了新生仔猪肠道的炎症反应。饲粮中添加SI在一定程度上可以缓解新生仔猪肠道受损。
This study was conducted to determine the effects of dietary oxidized fish oil (OFO) and the intervention of soybean isoflavones (SI) supplementation on growth performance, intestinal mucosal oxidation-related indicators, immune factors, and co-oxidation and immune-related factors in neonatal piglets, and to explore the effects of oxidative stress on growth and intestinal immune response in neonatal piglets. The neonatal piglets (n=96, 4 day old) were selected and divided into four groups. They were fed 5% fresh fish oil (FFO) (group Ⅰ), 5% FFO+50 mg/kg SI (group Ⅱ), 5% OFO (group Ⅲ), OFO+50 mg/kg SI (group Ⅳ). After 21 d of feeding, two piglets per replicate were slaughtered, and small intestines were sampled. The results were showed as follows: at the end of 21 d, body weight of the neonatal piglets in group Ⅲ was significantly decreased compared with that in group Ⅱ (P<0.05). From 1 to 21 d, daily weight gain of the neonatal piglets in group Ⅲ was significantly lower than that in group Ⅰ and group Ⅱ (P<0.05). Compared with group Ⅰ and group Ⅱ, the content of malondialdehyde (MDA) in the intestinal mucosa of piglets in group Ⅲ was increased by 90.89% (P<0.05) and 73.28% (P<0.05), respectively. SI supplementation significantly improved the activity of total superoxide dismutase (T-SOD) in intestinal mucosa (P<0.05). The OFO and SI produced a significant interaction effect on the activity of total anti-oxidation capability (T-AOC) in intestinal mucosa (P<0.01). The content of interleukin-2 (IL-2) in group Ⅲ was significantly higher than the that in group Ⅰ (P<0.01) and the contents of interleukin-8 (IL-8) (P<0.05) and interleukin-10 (IL-10) (P<0.01) in group Ⅲ were significantly lower than those in group Ⅰ(P<0.05), respectively. The OFO significantly increased the activity of inducible nitric oxide synthase (iNOS) (P<0.05) and the content of nuclear factor-κB (NF-κB) (P<0.01). Collectively, these results suggest that dietary OFO significantly reduces the antioxidant capacity and causes intestinal inflammation in piglets (4 to 24 d old). SI supplementation can alleviate intestinal damage of neonatal piglets.［Chinese Journal of Animal Nutrition, 2011, 23（5）：799 -806］
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