The study was conducted to investigate the effect of thyroxin (T4), pancreas glucagon (PG) and epidermal growth factor (EGF) on the mRNA expression level of oligopeptide transporter 1 (PepT1) in small intestine of broilers. Thirty broilers aged 25 days with similar body weight were randomly divided into 5 groups with 6 broilers in each group. Broilers from different groups were treated with different hormones once a day for 5 days. Broilers in control group, EGF group and PG group were subcutaneously injected distilled water at 0.20 mL/kg, EGF at 0.03 mg/kg and PG at 0.10 mg/kg, respectively; those in T4 group and TH group were drenched levothyoxine Na (T4) at 330.00 μg/kg and methimazole at 25.00 mg/kg, respectively, and subcutaneously injected distilled water at 0.20 mL/kg. After 12 h of the last treatment, the human epidermal growth factor (h-EGF), triiodothyronine (T3) and PG contents in serum were determined, as well as the mRNA expression level of PepT1 in duodenum, jejunum and ileum. The results showed as follows: there was no significant difference between the EGF group and PG group on the corresponding contents in serum (P>0.05); orally taken of T4 and methimazole significantly increased and decreased serum T3 content, respectively (P<0.05). The mRNA expression level of Pept1 in small intestine was significantly increased by subcutaneous injection of PG (P<0.05) and orally taken of T4 (P<0.01), but decreased by subcutaneous injection of EGF and orally taken of methimazole (P>0.05). The results indicate that thyroxin and PG play import roles in regulation of peptides absorption in broilers.［Chinese Journal of Animal Nutrition, 2011, 23（7）：1161 -1166］
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