The experiment was conducted to investigate the effect of cyclic high temperatures on the Zn2+ concentration and metallothionein content in pancreas, liver and duodenum of broiler chickens. One hundred and twenty 32-day-old Arbor Acres male broilers were randomly allotted to 3 groups (normal-temperature group, paired-feeding group and high-temperature group) with 4 replicates in each group and 10 birds in each replicate. The broilers in the normal-temperature group and paired-feeding group were maintained in a controlled environment temperature at 23 ℃， and the paired-feeding group were matched the diet intake of broilers in high-temperature group at the previous day of the experiment. The broilers in the high-temperature group were housed in a controlled environment temperature with daily cyclic high temperature (23 ℃-33 ℃－23 ℃), and drinked ad libitum. The results showed as follows: after 4 and 8 d cyclic high temperatures, the Zn2+ concentration in plasma, liver, pancreas and duodenum of broilers was significantly decreased (P＜0.05), and the mRNA expression level of metallothionein gene in pancreas was significantly increased (P＜0.05). After 4 d cyclic high temperatures, the metallothionein content and its mRNA expression level in liver and duodenum of broilers were significantly decreased (P＜0.05), after 8 d cyclic high temperatures, they became the normol levels, but the metallothionein content in pancreas was significantly decreased (P＜0.05); there was no significant differences in the Zn2+ concentration and metallothionein content, and the mRNA expression level of metallothionein gene between the normal-temperature group and paired-feeding group (P＞0.05). In conclusion, cyclic high temperatures significantly decreases the Zn2+ concentration and metallothionein content in liver, pancreas and duodenum of broilers, which is independent of the decreasing of food intake induced by cyclic high temperatures, and the changing trend of metallothionein content is similar with that of zinc concentration in liver, pancreas and duodenum of broilers.［Chinese Journal of Animal Nutrition, 2011, 23（8）：1273 -1279］
［1］PROUDFOOT F G, HULAN H W. Nutritive value of triticale as a feed ingredient for broiler chickens[J]. Poultry Science, 1988, 67:1743-1749.
[2]LEESON S, SUMMERS J D. Response of White Leghorns to diets containing ground or whole triticale[J]. Canadian Journal of Animal Science, 1987, 67(2):583-585.
[3]MCNAUGHTON J L, REECE F N. Response of broiler chickens to dietary energy and lysine levels in a warm environment[J]. Poultry Science, 1984, 63:1170-1174.
[4]李静，乔健，高铭宇，等.37 ℃持续热应激对肉鸡动脉压的影响[J].畜牧与兽医，2006，38(11)：4-6.
[5]杜荣，张敏红，张卫红，等.急性热应激对肉鸡血浆及尿中的矿物质浓度的影响[J].畜牧兽医学报，2000，31(4)：311-316.
[6]WOELFEL R L, OWENS C M, HIRSCHLER E M, et al. The characterization and incidence of pale, soft, and exudative broiler meat in a commercial processing plant[J]. Poultry Science, 2002, 81(4):579-584.
[7]徐振华，李褔昌，秦应和.日粮锌水平对生长肉兔生产性能、血清肝脏抗氧化酶活性和金属硫蛋白-1基因表达的影响[J].动物营养学报，2008,20(3)：337-342.
[8]王全溪，邵良平，林树根，等.日粮锌水平对雏番鸭免疫器官中细胞凋亡及表达的影响[J].动物营养学报，2008,20(5)：509-514.
[9]王建枫，孙建义，翁晓燕，等.日粮锌对大鼠肝脏脂肪酸代谢的影响[J].动物营养学报，2008,20(5)：586-591.
[10]BARTLETT J R, SMITH M O. Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress[J]. Poultry Science, 2003, 82(10):1580-1588.
[11]SUNDER G S, PANDA A K, GOPINATH N C S, et al. Effects of higher levels of zinc supplementation on performance, mineral availability, and immune competence in broiler chickens[J]. The Journal of Applied Poultry Research, 2008, 17:79-86.
[12]JAMES C F, RODNEY R D, CHARLE CM, et al. Tissue-specific accumulation of metallothionein in chickens as influenced by the route of zinc administration[J]. The Journal of Nutrition, 1988, 118:176-182.
[13]TOMOK K, NORIO I. Function of metallothionein in gene expression and signal transduction: newly found protective role of metallothionein[J]. Journal of Health Science, 2008, 54(3):251-260.
[14]JAMES C F, RODNEY R D, CHARLESh C M, et al. Ron-induced metallothionein in chick liver: a rapid, route-dependent effect independent of zinc status[J].The Journal of Nutrition, 1990, 120:1214-1222.
[15]BORGHESI L A, LYNES M A. Stress proteins as agents of immunological change: some lessons from metallothionein[J].Cell Stress ＆ Chaperones, 1996, 1(2)：99-108.
[16]COYLE P, PHILCOX J C, CAREY L C, et al. Metallothionein: the multipurpose protein[J]. Cellular and Molecular Life Sciences, 2002, 59:627-647.
[17]FERNANDO L P, WEI D, ANDREWS G K. Structure and expression of chicken metallothionein[J]. Journal of Nutrition, 1989, 119(2):309-318.
[18]HAQA F, MAHONEY M, KOROPATNICK J. Signaling events for metallothionein induction[J]. Mutation Research, 2003, 533:211-226.
[19]MCCORMICK C C. Induction and accumulation of metallothionein in liver and pancreas of chicks given oral zinc: a tissue comparison[J]. The Journal of Nutrition, 1984, 114(1):191-203.
[20]SCHROEDER J J, COUSINS R J. Interleukin 6 regulates metallothionein gene expression and zinc metabolism in hepatocyte monolayer cultures[J]. Proceedings of the National Academy of Sciences of the United States of America, 1990, 87(8):3137-3141.
[21]ARIZONO K, KAGAWA S, HAMADA H, et al. Nitric oxide mediated metallothionein induction by lipopolysaccharide[J]. Research Communications in Molecular Pathology and Pharmacology, 1995, 90(1):49-58.
[22]DE S K, MCMASTER M T, ANDREWS G K. Endotoxin induction of murine metallothionein gene expression[J]. The Journal of Biological Chemistry, 1990, 265(25):15267-15274.
[23]BAUMAN J W, MADHU C, MCKIM J M, Jr, et al. Induction of hepatic metallothionein by paraquat[J]. Toxicology and Applied Pharmacology, 1992, 117:233-241.
[24]BAUMAN J W, LIU Y P, ANDREWS G K, et al. Examination of potential mechanism(s) of metallothionein induction by diethyl maleate[J]. Toxicology and Applied Pharmacology, 1992, 117:226-232.
[25]BAUMAN J W, MCKIM J M, Jr, LIU J, et al. Induction of metallothionein by diethyl maleate[J]. Toxicology and Applied Pharmacology, 1992, 114:188-196.
[26]BAUMAN J W, LIU J, LIU Y P, et al. Increase in metallothionein produced by chemicals that induce oxidative stress[J]. Toxicology and Applied Pharmacology, 1991, 110(2):347-354.
[27]MIN K S, TERANO Y, ONOSAKA S, et al. Induction of hepatic metallothionein by nonmetallic compounds associated with acute-phase response in inflammation[J]. Toxicology and Applied Pharmacology, 1991, 111:152-162.
[28]吴大亮，杨开友.食品湿法消化中几个问题的探讨[J].海峡预防医学杂志，1996，2(2)：46.
[29]郭亚东，陈克嶙.火焰原子吸收光谱法测定生物样品中锌、铜和锰[J].光谱实验室，2006，23(3)：463-464.
[30]SCHEUHAMMER A M, CHERIAN M G. Quantification of metallothioneins by a silver-saturation method[J]. Toxicology and Applied Pharmacology, 1986, 82(3):417-425.
[31]SAHIN K, SMITH M O, ONDERCI M, et al. Supplementation of zinc from organic or inorganic source improves performance and antioxidant status of heat-distressed quail[J]. Poultry Science, 2005, 84:882-887.
[32]SAHIN K, KUCUK O. Zinc supplementation alleviates heat stress in laying Japanese quail[J]. Journal of Nutrition, 2003, 33:2808-2811.
[33]MORENG R E, BALNAVE D, ZHANG D. Dietary zinc methionine effect on eggshell quality of hens drinking saline water[J]. Poultry Science, 1992, 71:1163-1167.
[34]BALNAVE D, ZHANG D. Response of laying hens on saline drinking water to dietary supplementation with various Zn compounds[J]. Poultry Science, 1993, 72:603-609.
[35]SAHIN K, SAHIN N, KUCUK O, et al. Role of dietary zinc in heat-stressed poultry: a review[J]. Poultry Science, 2009, 88(10):2176-2183.
[36]DING H Q, ZHOU B, LIU L, et al. Oxidative stress and metallothionein expression in the liver of rats with severe thermal injury[J]. Burns, 2002, 28(3):215-221.
[37]LIBERGE M, BARTHLMY M R. Localization of metallothionein, heat shock protein (Hsp70), and superoxide dismutase expression in Hemidiaptomus roubaui (Copepoda, Crustacea) exposed to cadmium and heat stress[J]. Zool, 2007, 85(3):362-371.
[38]TAMAI K T, LIU X, SILAR P, et al. Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways[J]. Molecular and Cellular Biology, 1994, 14(12):8155-8165.
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