本文旨在初步探讨不同ω-6/ω-3多不饱和脂肪酸（PUFA）比例对扬州鹅血脂、血清超敏C反应蛋白（hypersensitive C-reactive protein, HsCRP）及可溶性CD40配体（sCD40L）的影响。试验选择160只同批出雏、体重接近的21日龄的扬州鹅，随机分成4组，每组4个重复，每个重复10只。分别饲喂ω-6/ω-3 PUFA比例为3∶1、6∶1、9∶1、12∶1的饲粮,并分别于42、56、70日龄时进行采血和血脂等指标的测定。结果表明：56、70日龄时，ω-6/ω-3 PUFA比例为6∶1组扬州鹅的血清中有较低的总甘油三酯（TG）（P<0.05）、总胆固醇（TC）（P<0.05）和低密度脂蛋白（LDL）（P<0.05），且该组的高密度脂蛋白（HDL）在4组中最高（P<0.05）。HsCRP或sCD40L水平总体上随ω-6/ω-3 PUFA比例的增加呈现上升趋势，基本都以3∶1组最低，但各处理间的差异多不显著（P＞0.05）。综上所述，ω-6/ω-3 PUFA比例为6∶1组有较好的降低血脂的效应，而低ω-6/ω-3 PUFA比例（3∶1）饲粮有降低扬州鹅HsCRP和sCD40L的趋势。
The objective of this trial was to investigate the effect of different ω-6/ω-3 PUFA ratios on blood lipid metabolism, and predictive factors for coronary artery disease in growing Yangzhou geese. Hundred and sixty 21-day-old healthy Yangzhou geese with average body weight of (0.407 0.023) kg were randomly divided into 4 groups. They were fed diets with different ω-6/ω-3 PUFA ratios, which were 3∶1, 6∶1, 9∶1 and 12∶1, respectively. The blood samples were obtained to determine the blood lipid, hypersensitive C-reactive protein (HsCRP), and soluble CD40 ligand (sCD40L) at the age of 42, 56, and 70 days, respectively. The results showed as follows: the geese fed the diet with the ω-6/ω-3 PUFA ratio of 6∶1 had much lower blood total triglyceride (TG) (P<0.05), total cholesterol (TC) (P<0.05), low-density lipoprotein (LDL) (P<0.05), and the highest high-density lipoprotein (HDL) (P<0.05) among the 4 groups at the age of 56 or 70 days. It was further observed that blood HsCRP or sCD40L levels were increased along with the ω-6/ω-3 PUFA ratio increasing, and generally were all the lowest in 3∶1 group, although no significant differences were found among the 4 groups (P>0.05). In conclusion, the diet with ω-6/ω-3 PUFA ratio of 6∶1 can decrease blood lipid; moreover, the diet with low ω-6/ω-3 PUFA ratios (3∶1) has a tendency to decrease blood HsCRP and sCD40L levels in Yangzhou geese.［Chinese Journal of Animal Nutrition, 2011, 23（8）：1289 -1295］
［1］RACKLEY C E. New clinical markers predictive of cardiovascular disease: the role of inflammatory mediators[J]. Cardiology in Review, 2004, 12(3):151-157.
[2]YUAN G, AI-SHALI K Z, HEGELE R A. Hypertriglyceridemia: its etiology, effects and treatment[J]. Canadian Medical Association Journal, 2007, 176(8):1113-1120.
[3]ALBERT C M, MA J, RIFAI N, et al. Prospective study of C-reactive protein, homocysteine, and plasma lipid levels as predictors of sudden cardiac death[J]. Circulation, 2002, 105(22): 2595-2599.
[4]AZIZ N, FAHEY J L, DETEL R, et al. Analytical performance of a high sensitive C-reactive protein based immunoassay and the effects of laboratory variables on levels of protein in blood[J]. Clinical and Diagnostic Laboratory Immunology, 2003, 10:652-657.
[5]SCHOENBECK U, LIBBY P. CD40 signaling and plaque instability[J]. Circulation Research, 2001, 89(12):1092-1103.
[6]SCHONBECK U, SUKHOVS G K, SHIMIZU K, et al. Inhibition of CD40 signaling limits evolution of established atherosclerosis in mice[J]. Proceedings of the National Academy of Sciences, USA., 2000, 97(13):7458-7463.
[7]ZHU Z R, AGREN J, MANNISTO S, et al. Fatty acid composition of breast adipose tissue in breast cancer patients and in patients with benign breast disease[J]. Nutrition & Cancer, 1995, 24(2):151-160.
[8]BALDASSARRE D, AMATO M, ELIGINI S, et al. Effect of n-3 fatty acids on carotid atherosclerosis and haemostasis in patients with combined hyperlipoproteinemia: a double-blind pilot study in primary prevention[J]. Annals of Medicine, 2006, 38(5):367-375.
[9]HJERKINN E M, ABDELNOOR M, BREIVIK L, et al. Effect of diet or very long chain omega-3 fatty acids on progression of atherosclerosis, evaluated by carotid plaques, intima-media thickness and by pulse wave propagation in elderly men with hypercholesterolaemia[J]. European Journal of Cardiovascular Prevention & Rehabilitation, 2006, 13(3):325-333.
[10]陈燕芬.ω-3多不饱和脂肪酸对脂代谢的影响及其抗炎抗心律失常作用的相关研究[D].硕士学位论文.南京：南京医科大学，2007．
[11]KATO T, HANCOCK R L, MOHAMMADPOUR H, et al. Influence of omega-3 fatty acids on the growth of human colon carcinoma in nude mice[J]. Cancer Letter, 2002, 187(2):169-177.
[12]张弛，肖俊霞，席守民，等.NO-1886降低脂负荷新西兰兔血清甘油三酯和游离脂肪酸[J]．中国药理学通报，2005，3:280-282．
[13]麻丽坤，尹兆正，谭利伟，等.日粮不同水平ω-3多不饱和脂肪酸对蛋鸡脂质代谢和免疫功能的影响[J]．中国兽医学报，2007，5:760-764．
[14]汪鲲，高占峰.富含n-3 PUFA蛋黄保健作用的研究[J]．饲料工业，2000，21(8):1-4．
[15]NAKANISHI T, OIKAWA D, KOUTOKU T, et al. Gamma-linolenic acid prevents conjugated linoleic acid-induced fatty liver in mice[J]. Nutrition, 2004, 20:390-393.
[16]时皎皎，糜漫天，韦娜．不同脂肪酸构成比对大鼠血脂影响的研究[J]．第三军医大学学报，2007，29(9):824-827．
[17]喻礼怀，王梦芝，王洪荣，等.能量水平对6~10扬州鹅增重效率及脂肪代谢的影响[J]．中国畜牧杂志，2010，46(21):28-32．
[18]ELABBASSI W, AL-NOORYANI A. Acute coronary syndrome. An acute inflammatory syndrome[J]. Saudi Medical Journal, 2006, 27(12):1799-1803.
[19]MATSUSHITA K, YATSUYA H, TAMAKOSHI K. Epidemiology of acute coronary syndrome[J]. Japanese Journal of Clinical Medicine, 2006, 64(4):625-632.
[20]CIPOLLONE F, CHIARELLI F, DAVI G, et al. Enhanced soluble CD40 ligand contributes to endothelial cell dysfunction in vitro and monocyte activation in patients with diabetes mellitus: effect of improved metabolic control[J]. Diabetologia, 2005, 48(6):1216-1224.
[21]樊一笋,朱华亭,李文香,等.可溶性CD40配体酶联检测试剂盒的研制及其运用[J].中国免疫学杂志，2004，20(7):480-482.
[22]蒋兴亮，周京国，唐中.脑梗死患者血浆sCD40L和可溶性血管黏附分子水平的变化及意义[J].中国现代医学杂志,2007,17(1):1595-1598.
[23]LEE J Y, PLAKIDAS A, LEE W H, et al. Differential modulation of toll-like receptors by fatty acids: preferential inhibition by n-3 polyunsaturated fatty acids[J]. Journal of Lipid Research, 2003, 44(3):479-486.
[24]VALERIO S, PASQUALE P, LUISA L, et al. Short-term treatment with atorvastatin reduces platelet CD40 ligand and thrombin generation in hypercholesterolemic patients[J]. Circulation, 2005, 111(4):412-419.
京公网安备 11010802041926号