动物营养学报 >

2011 , Vol. 23 >Issue 11: 1946 - 1952

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.11.015

分子营养

饲粮能量水平对乌金猪脂肪组织脂类分解代谢相关基因表达的影响

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  • 云南农业大学云南省动物营养与饲料重点实验室,昆明 650201
潘洪彬(1974-),吉林前郭人,博士研究生,从事动物营养与饲料科学研究。E-mail: ynsdyz@163.com

收稿日期: 2011-05-11

  网络出版日期: 2011-11-21

基金资助

云南省自然科学基金重点项目(2005C0008Z)

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Dietary Energy Level Affects Lipid Catabolism-Related Gene Expression in Adipose Tissue of Wujin Pigs

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  • Yunnan Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming 650201, China

Received date: 2011-05-11

  Online published: 2011-11-21

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摘要

本试验旨在研究饲粮不同能量水平对乌金猪脂肪组织脂类分解代谢相关基因表达的影响。选取体重约15 kg的乌金猪54头,随机分为3组,每组3个重复,每个重复6头猪,分别饲喂消化能为11.74(低能组)、12.89(中能组)和14.22 MJ/kg(高能组)的饲粮,在体重30、60和100 kg时屠宰取皮下脂肪组织,提取总RNA,荧光定量PCR法检测脂肪组织脂肪分解代谢相关酶和因子 基因的表达水平。结果表明:饲喂高能量饲粮乌金猪在30和100 kg体重时,脂肪组织HSL、CPT-Ⅰ和PPARγ基因的表达水平显著下降(P<0.05),LPL基因的表达水平显著升高(P<0.05);在60 kg体重时,4种基因的表达水平均显著升高(P<0.05)。结果提示,高能量饲粮可降低乌金猪在30和100 kg体重时脂肪的分解和脂肪酸的β-氧化,增强60 kg体重时脂肪的分解和脂肪酸的β-氧化。

关键词： 乌金猪; 饲粮能量水平; 脂肪组织; 脂肪分解代谢; 基因表达

本文引用格式

潘洪彬, 王静, 黄英, 赵素梅, 张曦, 葛长荣, 高士争 . 饲粮能量水平对乌金猪脂肪组织脂类分解代谢相关基因表达的影响[J]. 动物营养学报, 2011 , 23(11) : 1946 -1952 . DOI: 10.3969/j.issn.1006-267x.2011.11.015

Abstract

The aim of this study was to investigate the effects of dietary energy level on the expression of lipid catabolism-related genes in adipose tissue of Wujin pigs. Fifty-four Wujin pigs with body weight of 15 kg were randomly assigned into 3 groups with 3 replicates per group and 6 heads in each replicate. Pigs in low digestive energy group (11.74 MJ/kg, LDE group), middle digestive energy group (12.89 MJ/kg, MDE group) and high digestive energy group (14.22 MJ/kg, HDE group) were fed diets with three different digestive energy levels. At the body weight of 30, 60 and 100 kg, pigs were slaughtered and subcutaneous adipose tissue was collected for analysis of gene expression levels of lipid catabolism-related enzymes and factors by real-time PCR. The results showed as follows: at the body weight of 30 and 100 kg, the gene expression levels of HSL, CPT-Ⅰ and PPARγ in adipose tissue of Wujin pigs fed high energy level diet significantly decreased (P<0.05), while the gene expression level of LPL significantly increased(P<0.05); however, at the body weight of 60 kg, the expression levels of the four genes were significantly increased (P<0.05). The results indicate that high dietary energy level can decrease lipid catabolism and fatty acid β-oxadation of Wujin pigs at the body weight of 30 and 100 kg, but enhance lipid catabolism and fatty acid β-oxadation of Wujin pigs at the body weight of 60 kg.

Key words： Wujin pigs; dietary energy level; adipose tissue; lipid catabolism; gene expression

参考文献

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