动物营养学报 >

2011 , Vol. 23 >Issue 11: 1953 - 1960

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.11.016

饲料营养

成年母猪后肠木聚糖降解菌的筛选、鉴定及其对饲草纤维降解作用的研究

展开
  • 1. 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193;
    2. 南京农业大学动物科技学院, 南京 210095;
    3. 西南民族大学生命科学与技术学院,成都 610041
董杰丽(1982-),女,河北衡水人,硕士研究生,从事动物营养与饲料科学研究。E-mail: dongjieli-001@163.com

收稿日期: 2011-06-10

  网络出版日期: 2011-11-21

基金资助

中央级公益性科研院所基本科研业务费专项资金项目"成年猪后肠厌氧菌木聚糖酶基因克隆及其异源表达的研究"(2010jc-5-2);公益性行业(农业)科研专项经费专项"奶牛良种扩繁技术与产业化开发"(nyhyzx07-036)

收起

Xylan-Degrading Bacteria from the Hindgut of Adult Sows: Screening, Identification and Effects on Degradation of Forage Fiber

Expand
  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science, Nanjing Agriculture University, Nanjing 210095, China;
    3. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China

Received date: 2011-06-10

  Online published: 2011-11-21

Fold

摘要

本研究旨在通过对内蒙古黑猪成年母猪后肠木聚糖降解菌的分离、筛选及鉴定,并对比研究筛选菌和瘤胃液对饲草纤维降解的作用,获得可高效降解木聚糖的菌株。以木聚糖为唯一碳源,根据筛选平板上菌株透明圈大小和发酵液中木聚糖酶活性分离得到了2株活性较高的木聚糖降解菌(分别命名为ZF和MZ)。经形态学观察和16S rDNA核苷酸序列分析表明,菌株ZF和MZ分别为兼性厌氧产黄纤维单胞菌(Cellulomonas flavigena)和类芽孢杆菌(Paenibacillus sp.)。饲草纤维降解结果表明:瘤胃液对苜蓿和青贮玉米的中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)降解率均高于筛选菌;菌株ZF和MZ对3种饲草NDF和ADF降解率均有随发酵时间延长而增加的趋势,其中菌株ZF降解羊草ADF和NDF的效果等同甚至优于瘤胃液。结果提示,从成年母猪后肠中筛选的木聚糖降解菌株ZF和MZ可有效降解饲草中的纤维类物质,可作为生产饲用木聚糖酶的候选菌株。

关键词： 成年母猪; 木聚糖降解菌; 筛选; 鉴定; 纤维降解

本文引用格式

董杰丽, 张铁鹰, 齐志国, 吴兴群, 欧阳张智 . 成年母猪后肠木聚糖降解菌的筛选、鉴定及其对饲草纤维降解作用的研究[J]. 动物营养学报, 2011 , 23(11) : 1953 -1960 . DOI: 10.3969/j.issn.1006-267x.2011.11.016

Abstract

This experiment was conducted to study the isolation, screening and identification of efficient xylan-degrading bacteria from the hindgut of high roughage-resistance Inner Mongolia black adult sows, and to compare the effects of screening bacteria and rumen liquid on neutral detergent fiber (NDF) and acid detergent fiber (ADF) degradation of forages (alfalfa, Chinese wildrye and silage corn). Using xylan as the sole carbon source, two xylan-degrading bacteria named ZF and MZ were isolated by screening with the diameter of clearing zone and xylanase activity. Strains of ZF and MZ were identified as Cellulomonas flavigena and Paenibacillus sp., respectively, which were facultative anaerobe strains, by morphology observation and 16S rDNA sequence analysis. The degradation results showed that the degradabilities of NDF and ADF in alfalfa and silage corn by rumen fluid were higher than those by strains of ZF and MZ. With fermentation time prolonging, the degradabilities of NDF and ADF in Chinese wildrye by strain ZF and MZ had a tendency to increase, and the degradabilities of NDF and ADF in Chinese wildrye by strain ZF were equal or higher than those by rumen fluid. In conclusion, xylana-degrading bacteria ZF and MZ isolated from the hindgut of adult sows have efficient ability to degrade fiber; therefore, they can be used as candidate strains for xylanase production.

Key words： adult sows; xylan-degrading bacteria; screening; identification; fiber degradation

参考文献

[1] 张铁鹰,刘志伟,孙杰,等.植酸酶在肉仔鸡消化道内环境下活性变化的体外研究[J].畜牧兽医学报,2008,39(9):1212-1216.

[2] 孙建义,李卫芬,顾赛红.体外模拟动物胃肠条件下β-葡聚糖酶稳定性的研究[J].中国畜牧杂志,2002,38(1):18-19.

[3] GUO B, CHEN X L, SUN C Y, et al. Gene cloning, expression and characterization of a new cold-active and salt-tolerant endo-β-1,4-xylanase from marine Glaciecola mesophila KMM 241[J]. Applied Microbiology and Biotechnology, 2009, 84(6):1107-1115.  

[4] WU S J, LIU B, ZHANG X B. Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific[J]. Applied Microbiology and Biotechnology, 2006, 72:1210-1216.

[5] 刘辉.1株产碱性木聚糖酶菌株的筛选、产酶条件与酶学性质研究及其木聚糖酶基因的克隆表达 .硕士学位论文.杨凌:西北农林科技大学,2007.

[6] POLIMAN D S, DANIELSON D M, PEO E R. Value of high fiber diets for gravid swine[J]. Journal of Animal Science, 1979, 48:1385-1393.

[7] 高巍,孟庆翔.生长育肥猪胃肠道正常厌氧菌群的数量和分区[J].中国农业大学学报,2000,5(5):88-93.

[8] 高巍,孟庆翔.生长育肥猪后肠纤维分解菌的数量及微生物发酵作用[J].动物营养学报,2002,14(2):33-36.

[9] VAREL V H, FRYDA S F. Cellulolytic bacteria from pig large intestine[J]. Applied Microbiology and Biotechnology, 1984, 47(1):219-221.

[10] VAREL V H, POND W G. Characteristics of a new cellulolytic Clostridium sp. isolated from pig intestinal tract[J]. Applied and Environmental Microbiology, 1992,58(5) :1645-1649.

[11] VAREL V H, YEN J T, KREIKEMEIER K K. Addition of cellulolytic clostridia to the bovine rumen and pig intestinal tract[J]. Applied and Environmental Microbiology, 1995, 61(3):1116-1119.

[12] 王书然.奶牛饲用酶制剂筛选及应用的研究 .硕士学位论文.呼和浩特:内蒙古农业大学,2008.

[13] 徐柏森,冯汀,刘刚.扫描电镜生物样品的快速制备方法研究[J].中国野生植物资源,2007,19(6):47-48, 51.

[14] SIMPSON H D, HAUFLER U R, DANIEL R M. An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga[J]. Biochemical Journal, 1991, 277:413-417.

[15] ODENYO A A, BISHOP R, ASEFA G, et al. Isolation and characterization of anaerobic cellulose-degrading bacteria from East African porcupine (Hystrix cristata)[J]. Anaerobe, 1999, 5:93-100.

[16] 张丹,许景钢,路伟明,等.低温降解纤维素的细菌的筛选及鉴定[J].东北农业大学学报,2008,39(1):17-23.

[17] 陈倩颖,胡子全,刘连生.6株解有机磷细菌的分离鉴定[J].安徽农业大学学报,2009,36(3):417-421.

[18] 孙振涛.产碱性木聚糖酶菌株的筛选及产酶条件研究 .硕士学位论文.泰安:山东农业大学,2007.

[19] 高巍,孟庆翔.瘤胃细菌、原虫和真菌降解植物细胞壁的相对贡献及其互作[J].中国农业大学学报,2003,8(5):98-104.

[20] 王雅倩,俞路,闫寒寒.瘤胃真菌对纤维降解的研究进展[J].饲料工业,2008,9(5):41-43.

[21] 刘海霞,刘大森,隋美霞,等.羊常用粗饲料干物质和粗蛋白的瘤胃降解特性研究[J].营养饲料,2010,46(21):37-42.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2