为了探讨N-氨甲酰谷氨酸(N-carbamylglutamate, NCG)对环江香猪生长性能、营养物质消化率及血浆游离氨基酸含量的影响,试验选用平均体重为(12.6±1.7) kg的环江香猪10头,随机分为2组,每组5个重复,每个重复1头猪,单笼饲养,分别饲喂基础饲粮和添加0.1% NCG的试验饲粮,试验期为10 d。每日记录采食量,分别在试验开始和结束时称取试猪空腹体重;试验第8~10天收集粪样,用外源指示剂(TiO2)法测定常规营养物质的表观消化率;试验第10天采集血样,测定血浆中生化指标和氨基酸含量。结果表明,与基础饲粮组相比,0.1%的NCG添加组平均日增重显著升高(P<0.05),料重比显著降低(P<0.05);粗蛋白质、粗脂肪和干物质表观消化率均有所升高(P>0.05);血浆尿素氮、血氨和低密度脂蛋白含量均显著降低(P<0.05),碱性磷酸酶活性显著升高(P<0.05);血浆总氨基酸、必需氨基酸、芳香族氨基酸、苯丙氨酸和蛋氨酸含量均显著升高(P<0.05)。上述结果提示,饲粮中添加0.1%的NCG可促进机体对营养物质的消化吸收,改善机体氨基酸平衡,从而提高环江香猪的生长性能。
This study was conducted to determine effects of N-carbamylglutamate (NCG) on growth performance, nutrient digestibility and plasma free amino acid concentrations in growing pigs. Ten Huanjiang mini-pigs with an average body weight (BW) of (12.6±1.7) kg were used and randomly assigned into two groups (five replicates with one pig per replicate), representing supplementation with 0 or 0.1% NCG in a basal diet for 10 days. Daily feed intake, initial and final BW were recorded, respectively; fecal samples were collected from day 8 to 10 for determining the digestibility of routine nutrients by TiO2 indicator; blood samples were collected on day 10 for analyzing biochemical parameters and concentrations of free amino acids (AA), respectively. Results showed that compared with the basal diet group, 0.1% NCG supplementation significantly increased the average daily gain (P<0.05), and decreased the ratio of gain to feed (P<0.05). The apparent digestibility of crude protein, crude fat and dry matter were increased (P>0.05). Plasma alkaline phosphatase activity was significantly increased (P<0.05), while the plasma concentrations of urea nitrogen, ammonia and low-density lipoprotein were significantly decreased (P<0.05). The plasma concentrations of total amino acid, essential amino acid, aromatic amino acid, phenylalanine and methionine of pigs fed the diet supplemented with 0.1% NCG were higher than those of pigs fed the basal diet (P<0.05). These findings suggest that 0.1% NCG supplementation can promote the digestibility of nutrients, improve AA balance, and then increase the growth performance, which may provide some scientific information for the application of NCG in diets of Huanjiang mini-pigs.
[1] 孔祥峰,印遇龙,伍国耀,等.猪功能性氨基酸营养研究进展[J].动物营养学报,2009,21(1):1-7.
[2] 印遇龙,吴信,唐志如,等.不同水平精氨酸衍生物对断奶仔猪生长性能及腹泻的影响[J].农业现代化研究,2008,29(6):723-725.
[3] 岳隆耀,王春平,谯仕彦.日粮中添加N-氨甲酰谷氨酸(NCG)对断奶仔猪生长的影响[J].饲料与畜牧,2010,(1):15-17.
[4] 刘俊峰,吴琛,孔祥峰,等.精氨酸对妊娠环江香猪胎儿生长发育的影响[J].中国农业科学,2011,44(5):1040-1045.
[5] NRC. Nutrient requirements of swine[S]. 10th ed. Washington, D. C.: National Academy Press, 1998.
[6] 汤文杰,孔祥峰,刘志强,等.日粮不同蛋白质水平对肥育宁乡猪养分消化率和氮能代谢的影响[J].动物营养学报,2008,20(4):458-462.
[7] 孔祥峰,印遇龙,黄瑞林,等.中药超微粉饲料添加剂对断奶仔猪生长性能和血清生化参数的影响[J].中国兽医学报,2008,28(2):184-188.
[8] 何庆华,印遇龙,孔祥峰,等.中草药超微粉对断奶仔猪氨基酸消化吸收的影响[J].天然产物研究与开发,2008,20:307-311.
[9] 王琤,瞿明仁,游金明,等.N-氨甲酰谷氨酸对断奶仔猪生长性能、养分消化率及血清游离氨基酸含量的影响[J].动物营养学报,2010,22(4):1012-1018.
[10] 王凤来,张曼夫,陈清明,等.日粮磷和钙磷比例对小型猪(香猪)血清、肠、骨碱性磷酸酶及血清钙磷的影响[J].动物营养学报,2008,13(1):36-42.
[11] SHARMAN M J, FERNANDEZ M L, ZERN T L, et al. Replacing dietary carbohydrate with protein and fat decreases the concentrations of small LDL and the inflammatory response induced by atherogenic diets in the guinea pig[J]. Journal of Nutritional Biochemistry, 2008, 19:732-738.
[12] CHEN H Y, MILLER P S, LEWIS A J, et al. Changes in plasma urea concentration can be used to determine protein requirements of two populations of pigs with different protein accretion rates[J]. Journal of Animal Science, 1995, 73:2631-2639.
[13] 周锡红,吴信,唐香山,等.不同水平精氨酸生素对断奶仔猪生长性能和血液指标的影响[J].农业现代化研究,2010,31(2):237-240.
[14] WU G, MORRIS S M, Jr. Arginine metabolism: nitric oxide and beyond[J]. Biochemistry Journal, 1998, 336:1-17.
[15] ZHAN Z F, OU D Y, PIAO X S. Dietary arginine supplementation affects microvascular development in the small intestine of early-weaned pigs[J]. The Journal of Nutrition, 2008, 138(7):1304-1309.
[16] 胡琴,印遇龙,孔祥峰,等.中药复方对早期断奶仔猪血清游离氨基酸含量的影响[J].广西农业生物科学,2008,27(1):55-59.
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