综述

生长动物脂肪代谢关键酶基因表达调控

展开
  • 1. 中国农业科学院饲料研究所,农业部饲料生物技术重点开放实验室,北京 100081;
    2. 赣南师范学院,赣州 341000
岳 颖(1988—),女,辽宁凤城人,硕士研究生,从事动物营养与饲料科学研究。E-mail: yueying8269@126.com

收稿日期: 2011-08-29

  网络出版日期: 2012-02-07

基金资助

国家肉鸡产业技术体系建设专项基金

Modulating the Expression of Genes Regulating Key Enzymes for Lipid Metabolism in Growing Animals

Expand
  • 1. Feed Research Institute,Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Gannan Normal University, Ganzhou 341000, China

Received date: 2011-08-29

  Online published: 2012-02-07

摘要

动物体内脂肪组织合成与分解代谢的调控主要是通过调控脂肪代谢关键酶的含量和活性来进行的。关键酶的基因表达水平决定了酶蛋白的多寡和活性的高低。本文对脂肪代谢中的脂肪酸合成酶(FAS)、乙酰辅酶A羧化酶(ACC)、激素敏感酯酶(HSL)、脂蛋白酯酶(LPL)的活性及其相应的基因表达水平影响脂肪代谢的研究进展进行综述。

本文引用格式

岳颖, 刘国华, 郑爱娟, 张华, 王晓方, 李婷婷, 卢占军 . 生长动物脂肪代谢关键酶基因表达调控[J]. 动物营养学报, 2012 , 24(2) : 232 -238 . DOI: 10.3969/j.issn.1006-267x.2012.02.007

Abstract

Synthesis and catabolism in animal adipose tissue are mainly regulated by key enzymes of lipid metabolism. Gene expression level determines the amount of enzymes and their activities. This article mainly reviews the progress in effects of gene expression of some key enzymes (fatty acid synthetase, acetylcoenzyme A, hormone-sensitive lipase and lipoproteinesterase) and their activities on lipid metabolism.

参考文献

[1] 胡兰.动物生物化学[M].北京:中国农业大学出版社,2007:176.



[2] DONKIN S S, CHIU P Y, YIN D, et al. Porcine somatotropin differentially down-regulates expression of the GLUT4 and fatty acid synthase genes in pig adipose tisse[J]. The Journal of Nutrition, 1996,126:2568-2577.



[3] 熊文中,杨凤,周安国.猪重组生长激素对不同杂交肥育猪脂肪代谢调控的研究[J].畜牧兽医学报,2001,32(1):1-4.



[4] MILDNER A M, CLARKE S D. Porcine fatty acid synthase: cloning of a complementary DNA, tissue distribution of its mRNA and suppression of expression by somatotropin and dietary protein[J]. The Journal of Nutrition, 1991,121:900-907.



[5] HARRIS D M, DUNSHEA F R, BAUMAN D E, et al. Effect of in vivo somatotropin treatment of growing pigs on adipose tissue lipogenesis[J]. Journal of Animal Science, 1993, 71(12):3293-3300.



[6] YIN D, CLARKE S D, PETERS J L, et al. Somatotropin-dependent decrease in fatty acid synthase mRNA abundance in 3T3-F442A adipocytes is the result of a decrease in both gene transcription and mRNA stability[J]. Biochemical Journal, 1998, 331:815-820.



[7] YIN D, CLARKE S D, ETHERTON T D. Transcriptional regulation of fatty acid synthase gene by somatotropin in 3T3-F442A adipocytes[J]. Journal of Animal Science, 2001, 9(9):2336-2345.



[8] 郝军,刘青娟,郑书深,等.不同浓度胰岛素对人肾小管上皮细胞SREBP1、FAS表达及脂质形成的影响[J].中国病理杂志,2010,26(7):1275-1279.



[9] 韩春春,王继文,符自英,等.葡萄糖和胰岛素对鹅肝细胞脂肪酸合成酶活性及转录表达的影响[J].农业生物技术学报,2009,17(4):634-637.



[10] PRIP-BUUS C, PERDEREAU D, FOUFELLE F, et al. Induction of fatty-acid-synthase gene expression by glucose in primary culture of rat hepatocytes[J]. European Journal of Biochemistry, 1995, 230:309-315.



[11] WANG D, SUL H S. Upstream stimulatory factor binding to the E-box at -65 is required for insulin regulation of the fatty acid synthase promoter[J]. Journal of Biological Chemistry, 1997, 272(42):26367-26374.  



[12] 颜新春,汪以真,许梓荣.动物脂肪酸合成酶(FAS)基因表达的调控[J].动物营养学报,2002,14(2):1-4.



[13] KIM T S, FRESKE H C. High carbohydrate diet and starvation regulate lipogenic mRNA in rats in a tissue-specific manner[J]. The Journal of Nutrition, 1996, 126(3):611-617.



[14] HASEGAWA J, OSATOMI K, WU R F, et al. A novel factor binding to the glucose response elements of liver pyruvate kinase and fatty acid synthase genes[J]. Journal of Biological Chemistry, 1999, 274(2):1100-1107.  



[15] 张英杰,刘月琴,刘景云.饲粮不同蛋白水平对绵羊脂肪和肌肉中FAS基因表达的影响[J].畜牧兽医学报,2010,41(7):829-834.



[16] BLARKE W L, CLARKE S D. Suppression of rat hepatic fatty acid syntehase and S14 gene transciption by dietary polyun-saturated fat[J]. The Journal of Nutrition, 1990, 120(12):1727-1729.



[17] CLARKE S D, ARMSTRONG M K, JUMP D. Nutritional control of rat liver fatty acid synthase and and S14 mRNA abundance[J]. The Journal of Nutrition, 1990, 120:218-224.



[18] CLARKE S D. Regulation of fatty acid synthase gene expression: an approach for reducing fat accumulation [J]. Journal of Animal Science, 1993, 71:1957-1965.



[19] SMITH D R, KNABE D A, SMITH S B. Depression of lipogenesis in swine adipose tissue by specific dietary fatty acids[J]. Journal of Animal Science, 1996, 74(5):975-983.



[20] 栾广春,王加启,卜登攀,等.多不饱和脂肪酸对机体脂肪代谢基因表达调控研究进展[J].中国畜牧兽医,2007,34(12):5-8.



[21] SAMPATH H, NTAMBI J M. Polyunsaturated fatty acid regulation of genes of lipid metabolism[J]. Annual Review of Nutrition, 2005, 25:317-340.



[22] XU J, NAKAMURA M T, CHO H P. Sterol regulatory element binding protein-1 expression is suppressed by dietary polyunsaturated fatty acids[J]. Journal of Biological Chemistry, 1999, 274:23577-23583.



[23] 刘作华,杨飞云,孔路军,等.饲粮能量水平对生长育肥猪肌内脂肪含量以及脂肪酸合成酶和激素敏感脂酶mRNA表达的影响[J].畜牧兽医学报,2007,38(9):934-941.



[24] 张英杰,李宗波,刘月琴,等.不同能量水平日粮对绵羊脂肪酸合成酶(FAS)基因表达的影响[J].中国草食动物,2010(z1):281-283.



[25] MABROUK G M, HELMY I M, THAMPY K G, et al. Acute hormonal control of acetyl-CoA carboxylase[J]. The Journal of Biological Chemistry, 1990, 265(11):6330-6338.



[26] COUSIN B, CASTEILLA L, DANI C, et al. Adipose tissues from various anatomical sites are characterized by different patterns of gene expression and regulation[J]. Biochemical Journal, 1993,292:873-876.



[27] LIU C Y, GRANT A L, KIM K H, et al. Porcine somatotropin decreases acetyl-CoA carboxylase gene expression in porcine adipose tissue[J]. Domestic Animal Endocrinology, 1994, 11(1):125-132.  



[28] IRITANI N, FUKUDA E, INOGUCHI K. A possible role of Z protein in dietary control of hepatic triacylglycerol synthesis. [J]. Journal of Nutritional Science and Vitaminology, 1980, 26(3):271-277.  



[29] STRALFORS P, BELFRAGE P. Phosphorylation of hormone-sensitive lipase by cyclic AMP-dependent protein kinase[J]. The Journal of Biochemical, 1983, 258:15146-15152.



[30] 邵明丽,许梓荣.猪脂肪代谢及其调控研究进展[J].饲料博览,2002(10):12-15.



[31] LAMPIDONIS A D, ROGDAKIS E, VOUTSINAS G E, et al. The resurgence of hormone-sensitive lipase (HSL) in mammalian lipolysis[J]. Gene, 2011,477:1-11.



[32] HOLM C, KIRCHGESSNER T G, SVENSON K L, et al. Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3[J]. Science, 1988, 241:1503-1506.



[33] ZHIGAO L, SUMIDA M, BIRCHBAUER A, et al. Isolation and characterization of the gene for mouse hormone-sensitive lipase[J]. Genomics, 1994, 24:259-265.



[34] SZTROLOVICS R, WANG S P, LAPIERRE P, et al. Hormone-sensitive lipase (Lipe): sequence analysis of the 129Sv mouse Lipe gene[J]. Mammalian Genome, 1997, 8(2):86-89.  



[35] FREDRIKSON G, STRACFORS P, NILSSON N O, et al. Hormone-sensitive lipase of rat adipose tissue. Purification and some properties[J]. The Journal of Biological Chemistry, 1981, 256(12):6311-6320.



[36] OSTERLUND T, DANIELSSON B, DEGERMAN E. Domain-structure analysis of recombinant rat hormone sensitive lipase[J]. Biochemical Journal, 1996,319:411-420.



[37] HARBITZ I, LANGSET M, EGE A G, et al. The porcine hormone sensitive lipase gene: sequence, structure, polymorphisms and linkage mapping[J]. Animal Genetics, 1999, 30(1):10-16.  



[38] CORDLE S R, COLBRAN R J, YEAMAN S J. Hormone-sensitive lipase from bovine adipose tissue[J]. Biochimca et Biophysica Acta: Molecular Cell Research, 1986, 887(1):51-57.  



[39] GARTON A J, CAMPBELL D G, COHEN P, et al. Primary structure of the site on bovine hormone sensitive lipase phosphorylated by cyclic AMP-dependent protein kinase[J]. FEBS Letters, 1988, 229(1):68-72.  



[40] LAMPIDONIS A D, STRAVOPODIS D J, VOUTSINAS G E, et al. Cloning and functional characterization of the 5' regulatory region of ovine Hormone[J]. Gene, 2008, 427:65-79.



[41] 孙世铎,杨在清,马志科,等.猪脂肪沉积的品系差异及其cAMP的调控作用[J].畜牧兽医学报,1996,27(6):489-494.



[42] 张幸开,万发春,王加启,等.影响激素敏感脂酶活性及基因表达的因素 //2004学术年会暨第五届全国畜牧兽医青年科技工作者学术研讨会论文集.北京:中国畜牧兽医学会,2004.



[43] 胡忠泽,金光明,刘海,等.茶多酚对肉鸡脂肪代谢的影响及作用机制[J].南京农业大学学报,2006(2):85-88.



[44] LANGFORT J, JAGSZ S, DOBRZYN P, et al. Testosterone affects hormone-sensitive lipase (HSL) activity and lipid metabolism in the left ventricle[J]. Biochemical and Biophysical Research Communication, 2010, 399(4):670-676.  



[45] AWAD A B, CHATTOPADHYAY J P. Effect of dietary saturated fatty acids on intracellular free fatty acids and kinetic properties of hormone-sensitive lipase of rat adipocytes[J]. The Journal of Nutrition, 1986, 116(6):1-95.



[46] SZTALRYD C, KRAEMER F B. Regulation of hormone-sensitive lipase during fasting[J]. American Journal of Physiology, 1994, 266(2):E179-E185.



[47] STICH V, HARANT I, GLISEZINSKI I D, et al. Adipose tissue lipolysis and hormone-sensitive lipase expression during very-low-calorie diet in obese female identical twins[J]. The Journal of Clinical Endocrinology and Metabolism, 1997, 82(3):739-744.  



[48] OGASAWARA J, NOMURA S, RAHMAN N, et al. Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes[J]. Biochemical and Biophysical Research Communication, 2010, 400:134-139.



[49] STROM K, GUNDERSEN T E, HANSSON O, et al. Hormone-sensitive lipase (HSL) is also a retinyl ester hydrolase: evidence from mice lacking HSL[J]. The FASEB Journal, 2009, 23(7):2307-2316.  



[50] DEEB S S, PENG R. Structure of human lipoprotein lipase gene[J]. Biochemistry, 1989, 28(10):4131-4135.  



[51] WION K L, KIRCHGESSNER T G, LUSIS A J, et al. Human lipoprotein lipsae complementary DNA sequence[J]. Science, 1987, 235:1638-1641.



[52] HARBITZ I, KRISTENSEN T, KRAN S, et al. Isolation and sequencing of porcine lipoprotein lipase cDNA and its use in multiallelic restriction fragment length polymorphism detection[J]. Animal Genetics, 1992, 23(6):517-522.



[53] RAISONNIER A, ETIENNE J, ARNAULT F. Comparison of the cDNA and amino acid sequences of lipoprotein lipase in eight species[J]. Comparative Biochemistry and Physiology, 1995, 111(3):385-398.



[54] 蒋瑞瑞,赵桂苹,陈继兰,等.爱拔益加肉鸡和北京油鸡脂肪代谢及其相关基因表达的比较研究[J].动物营养学报,2010,22(5):1334-1441.



[55] 谭正怀,莫正纪.脂蛋白脂酶的组织特异性表达及其调节[J].国外医学:内分泌学分册,2000,20(6):320-324.



[56] 刘蒙,宋代军,齐珂珂,等.饲粮代谢能水平对北京油鸡脂肪沉积和LPL基因表达的影响[J].中国畜牧兽医,2009,36(5):9-13.



[57] 廉红霞,卢德勋,高民.饲粮营养水平对猪血液生化指标、背最长肌IMF含量及LPL mRNA表达量的影响[J].中国饲料,2008(5):12-16.



[58] 郑珂珂,朱晓鸣,韩冬,等.饲料脂肪水平对瓦氏黄颡鱼生长及脂蛋白脂酶基因表达的影响[J].水生生物学报,2010,34(4):815-821.



[59] 梁旭方,白俊杰,劳海华.真鲷脂蛋白脂肪酶基因表达与内脏脂肪蓄积营养调控定量研究.海洋与湖沼[J].海洋与湖沼,2003,34(6):625-631.
文章导航

/