早期断奶仔猪饲粮丙氨酰谷氨酰胺适宜添加量的研究

袁雪波; 马黎; 陈克嶙; 陈恒灿; 郭荣富

doi:10.3969/j.issn.1006-267x.2012.04.007

动物营养学报 >

2012 , Vol. 24 >Issue 4: 631 - 637

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.04.007

猪与禽营养

早期断奶仔猪饲粮丙氨酰谷氨酰胺适宜添加量的研究

袁雪波 ,
马黎 ,
陈克嶙 ,
陈恒灿 ,
郭荣富

展开

1. 云南农业大学, 云南省动物营养与饲料重点实验室, 昆明 650201;
2. 云南农业职业技术学院, 昆明 650212

收稿日期: 2011-10-20

网络出版日期: 2012-03-30

基金资助

云南省教育厅科学研究基金项目(08y0396);云南生猪产业技术体系建设项目(A3006688)

收起

Dietary Adequate Supplementation of Alanyl-Glutamin for Early Weaner Piglets

YUAN Xuebo ,
MA Li ,
CHEN Kelin ,
CHEN Hengcan ,
GUO Rongfu

Expand

1. Yunnan Provincial Animal Nutrition and Feed Key Laboratory, Yunnan Agricultural University, Kunming 650201, China;
2. Yunnan Agricultural Vocational and Technical College, Kunming 650212, China

Received date: 2011-10-20

Online published: 2012-03-30

Fold

摘要

本文旨在研究丙氨酰谷氨酰胺(Ala-Gln)对早期断奶仔猪生长性能、血液生化指标和小肠黏膜酶活性的影响,探讨Ala-Gln在早期断奶仔猪饲粮中的适宜添加量。选用48头(公母各占1/2)纯种云南撒坝仔猪为试验对象,35日龄断奶,按单因素完全随机化设计分为4组,每组3个重复,每个重复4头猪。各组分别饲喂在基础饲粮上添加0(对照组)、0.15%、0.30%、0.45% Ala-Gln的试验饲粮,试验期为35~60日龄。结果表明:1)0.30%组显著提高了全期仔猪平均日增重和平均日采食量(P<0.05),随着Ala-Gln添加水平的提高,全期仔猪平均日增重和平均日采食量以二次曲线方式提高(P=0.004和P=0.006);2)0.30%组极显著提高了49日龄仔猪血清总蛋白、葡萄糖含量及谷丙转氨酶活性(P<0.01),显著提高了血清碱性磷酸酶活性(P<0.05),极显著降低了血清尿素氮含量(P<0.01),显著降低了乳酸脱氢酶活性(P<0.05)。各组血清白蛋白、肌酐含量及肌酸激酶活性均差异不显著(P>0.05)。3)试验组仔猪空肠黏膜钠泵(Na⁺-K⁺-ATPase)和总二糖酶活性均显著或极显著高于对照组(P<0.05,P<0.01)。结果提示,添加适量的Ala-Gln对早期断奶仔猪具有一定的促生长效应,在本试验条件下,依据二次曲线拟合结果,推荐Ala-Gln适宜添加量为0.27%。

关键词： 早期断奶仔猪; 丙氨酰谷氨酰胺; 适宜添加量; 生长性能; 钠泵

本文引用格式

袁雪波 , 马黎 , 陈克嶙 , 陈恒灿 , 郭荣富 . 早期断奶仔猪饲粮丙氨酰谷氨酰胺适宜添加量的研究[J]. 动物营养学报, 2012 , 24(4) : 631 -637 . DOI: 10.3969/j.issn.1006-267x.2012.04.007

Abstract

This experiment was conducted to investigate the effects of alanyl-glutamine (Ala-Gln) on growth performance, blood biochemical indices and jejunal mucosal enzyme activities of early weaner piglets, and to explore the dietary adequate supplementation of Ala-Gln for early weaner piglets. Forty eight pure breed Yunnan Saba piglets (half male, half female) weaned at 35 days of age were selected and randomly allocated into 4 groups with 3 replicates in each group and 4 piglets in each replicate. Diets of the four groups were supplemented with 0 (control group), 0.15%, 0.30% and 0.45% Ala-Gln in a basal diet, respectively. The duration of experiments was 35 to 60 days of age. The results showed as follows: 1) the average daily gain and feed intake were significantly increased by adding 0.30% Ala-Gln (P<0.05), with Ala-Gln supplementation levels increasing, total average daily gain and feed intake were improved in a quadratic fashion manner (P=0.004 and P=0.006); 2) at 49 days of age, the contents of serum total protein, glucose and alanine aminotransferase activity were significantly increased by adding 0.3% Ala-Gln (P<0.01), and the serum alkaline phosphatase activity was significantly increased (P<0.05), but the serum urea nitrogen content (P<0.01) and lactate dehydrogenase activity (P<0.05) were significantly lower than those in the control group (P<0.05). There were no significant differences in the contents of serum albumin, creatinine and creatine kinase activity between all groups (P>0.05). The activities of jejunal mucosal Na⁺-K⁺-ATPase and total disaccharidase in experimental groups were significantly higher than those in the control group (P<0.05, P<0.01). The results suggest that growth performance of early weaner piglets can be improved by adding Ala-Gln. Collectively, according to the result of quadratic fashion, the dietary adequate supplementation of Ala-Gln is 0.27% for early weaner piglets.

Key words： early weaner piglets; Ala-Gln; adequate supplementation; growth performance; Na⁺-K⁺-ATPase

参考文献

[1] WALLACE C,KEAST D.Glutamine and macrophage function[J].Metabolism,1992,41(9):1016-1020.

[2] REEDS P J,BURRIN D G,STOLL B,et al.Intestinal glutamate metabolism[J].The Journal of Nutrition,2000,130:978-982.

[3] MURAKAMI A E,SAKAMOTO M I,NATALI M R M,et al.Supplementation of glutamine and vitamin E on the morphometry of the intestinal mucosa in broiler chickens[J].Poultry Science,2007,86:488-495.

[4] YI G F,CARROLL J A,ALLEE G L,et al.Effect of glutamine and spray-dried plasma on growth performance,small intestinal morphology,and immune responses of Escherichia coli K88⁺-challenged weaned pigs[J].Journal of Animal Science,2005,83(3):634-643.

[5] 桑剑锋,吴文溪.丙氨酰谷氨酰胺二肽的代谢及在肠外营养中的应用[J].肠外与肠内营养,2001,8(1):46-49.

[6] 郭荣富.谷氨酰胺对隔离早期断奶仔猪的营养生理效应研究[D]博士学位论文.雅安:四川农业大学,2003:65-67.

[7] 袁雪波,马黎,陈克嶙,等.丙氨酰谷氨酰胺二肽对哺乳仔猪生长性能、小肠形态学和血清生化指标的影响[J].动物营养学报,2011,23(1):94-101.

[8] JIANG Z Y,SUN L H,LIN Y C,et al.Effects of dietary glycyl-glutamine on growth performance,small intestinal integrity,and immune responses of weaning piglets challenged with lipopolysaccharide[J].Journal of Animal Science,2009,87(12):4050-4056.

[9] 席鹏彬,林映才,蒋宗勇,等.谷氨酰胺二肽对断奶仔猪生长、免疫、抗氧化力和小肠黏膜形态的影响[J].动物营养学报,2007,19(2):135-141.

[10] 黄冠庆,傅伟龙,高萍,等.甘氨酰谷氨酰胺二肽对断奶仔猪生长性能及血液激素水平的影响[J].华南农业大学学报,2003,24(3):66-68.

[11] 曾翠平.甘氨酰谷氨酰胺对早期断奶仔猪的促生长作用及其机制[D]博士学位论文.广州:华南农业大学,2004:19-25.

[12] AZUMA T,GU W,MIZOE A,et al.Nutritional effects of alanyl-glutamine after liver transplantation in pigs[J].Transplantation Proceeding,1998,30:3705-3706.

[13] OBA M,BALDWIN R L,BEQUETTE B J.Oxidation of glucose,glutamate,and glutamine by isolated ovine enterocytes in vitro is decreased by the presence of other metabolic fuels[J].Journal of Animal Science,2004,82:479-486.

[14] FISHMAN W H.Alkaline phosphatase isozymes:recent progress[J].Clinical Biochemstry,1990,23:99-104.

[15] BRENNAN L,CORLESS M,HEWAGE C,et al.Newsholme P.¹³C NMR analysis reveals a link between L-glutamine metabolism,D-glucose metabolism and gamma-glutamyl cycle activity in a clonal pancreatic beta-cell line[J].Diabetologia,2003,46:1512-1521.

[16] ZOU X T,ZHENG G H,FANG X J,et al.Effects of glutamine on growth performance of weanling piglets[J].Czech Journal of Animal Science,2006(10):444-448.

[17] COOMBES J S,MCNAUGHTON L R.Effects of branched-chain amino acid supplementation on serum creatine kinase and lactate dehydrogenase after prolonged exercise[J].Journal of Sports Medicine and Physical Fitness,2000,40(3):240-246.

[18] UENO P M,ORIA R B,MAIER E A,et al.Alanyl-glutamine promotes intestinal epithelial cell homeostasis in vitro and in a murine model of weanling undernutrition[J].American Journal of Physiology Gastrointestinal and Liver,2011,301(4):G612-G622.

[19] 蒋宗勇,郑卫川,林映才,等.谷氨酰胺对新生仔猪生长性能、血清激素水平及小肠黏膜酶活性的影响[J].动物营养学报,2010,22(1):125-131.

[20] SAMULITIS-DOS S B K,GODA T,KOLDOVSKY O.Dietary-induced increases of disaccharidase activities in rat jejunum[J].British Journal of Nutrition,1992,67(2):267-278.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们