动物营养学报 >

2012 , Vol. 24 >Issue 4: 638 - 645

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.04.008

猪与禽营养

43~63日龄黄羽肉鸡核黄素需要量研究

  • 阮栋 ,
  • 蒋守群 ,
  • 周桂莲 ,
  • 陈芳 ,
  • 洪平
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  • 广东省农业科学院畜牧研究所, 农业部动物营养与饲料(华南)重点开放实验室, 广州 510640

收稿日期: 2011-12-01

  网络出版日期: 2012-03-30

基金资助

公益性行业(农业)科研专项(200903006);国家肉鸡产业技术体系项目(CARS-42)

收起

Riboflavin Requirement of Yellow-Feathered Broilers Aged 43 to 63 Days

  • RUAN Dong ,
  • JIANG Shouqun ,
  • ZHOU Guilian ,
  • CHEN Fang ,
  • HONG Ping
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  • Institute of Animal Science, Guangdong Academy of Agricultural Sciences, The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou 510640, China

Received date: 2011-12-01

  Online published: 2012-03-30

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摘要

本试验旨在研究饲粮中添加不同水平核黄素对43~63日龄黄羽肉鸡生长性能、血液和肝脏生化指标及肉质性状的影响,探讨43~63日龄黄羽肉鸡核黄素需要量。试验选用43日龄岭南黄羽肉公鸡1 200只,采用单因子随机分组试验设计,随机分为5组,每组6个重复,每个重复40只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加1.5、3.0、6.0和12.0 mg/kg核黄素的试验饲粮,试验期21 d。结果表明:与对照组相比,添加3.0 mg/kg核黄素显著提高了试验鸡平均日增重(P<0.05),各组间料重比差异不显著(P>0.05)。饲粮添加核黄素显著降低了血清氧化型谷胱甘肽(GSSG)含量,并提高了还原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)值(P<0.05),其中以3.0 mg/kg添加组GSH/GSSG值最高;添加6.0 mg/kg核黄素显著提高了试验鸡肝脏黄嘌呤氧化酶(XOD)活性(P<0.05);试验鸡血浆核黄素含量随着饲粮核黄素添加水平的增加而呈递增趋势,当添加水平为12.0 mg/kg时与对照组差异显著(P<0.05)。饲粮添加核黄素对试验鸡脾脏指数、法氏囊指数、胸腺指数、血液T淋巴细胞增殖效应以及碱性磷酸酶(ALP)活性影响均不显著(P>0.05)。饲粮添加3.0 mg/kg核黄素显著提高了胸肌红度值(P<0.05),添加6.0 mg/kg核黄素则显著降低了胸肌亮度值(P<0.05);添加核黄素对试验鸡胸肌pH、滴水损失及胸肌黄度值无显著影响(P>0.05)。综上所述,以平均日增重和抗氧化性能为评定指标,43~63日龄黄羽肉鸡核黄素适宜添加水平为3.0 mg/kg。

关键词： 核黄素; 黄羽肉鸡; 需要量

本文引用格式

阮栋 , 蒋守群 , 周桂莲 , 陈芳 , 洪平 . 43~63日龄黄羽肉鸡核黄素需要量研究[J]. 动物营养学报, 2012 , 24(4) : 638 -645 . DOI: 10.3969/j.issn.1006-267x.2012.04.008

Abstract

This experiment was conducted to study the effects of dietary riboflavin levels on growth performance, biochemical indices of blood and liver, and meat quality of yellow-feathered broilers aged 43 to 63 days, and then to estimate the riboflavin requirement of yellow-feathered broilers. One thousand five hundred 43-day-old Lingnan male broilers were randomly assigned to 5 dietary groups with 6 replicates per group and 40 birds per replicate with the single factorial arrangement design. Broilers in control group were fed a basal diet and broilers in other groups were fed the basal diet supplemented with 1.5, 3.0, 6.0 and 12.0 mg/kg riboflavin, respectively. The experiment lasted for 21 days. The results showed as follows: compared with the control group, adding 3.0 mg/kg riboflavin significantly increased the average daily gain (ADG) of broilers (P<0.05), but there was no difference in the ratio of feed to gain (F/G) among all groups (P>0.05). Adding riboflavin significantly decreased serum oxidized glutathione (GSSG) content and significantly increased the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) (P<0.05), and the highest GSH/GSSG was in 3.0 mg/kg group. Adding 6.0 mg/kg riboflavin significantly increased the xanthine oxidase (XOD) activity of liver (P<0.05). The plasma riboflavin content was increased with the dietary riboflavin addition, and 12.0 mg/kg group was significantly higher than the control group (P<0.05). There was no difference in spleen index, bursa of Fabriciu index, thymus index, proliferation of T-cell effect and alkaline phosphatase (ALP) activity among all groups (P>0.05). Compared with the control group, adding 3.0 mg/kg riboflavin significantly increased the a* value (P<0.05), adding 6.0 mg/kg riboflavin significantly decreased L* value (P<0.05), but adding riboflavin did not affect the pH, drip loss and b* value of breast muscle (P>0.05). In conclusion, based on ADG and antioxidant function, the optimal riboflavin level is 3.0 mg/kg of yellow-feathered broilers aged 43 to 63 days.

Key words： riboflavin; yellow-feathered broilers; requirement

参考文献

[1] OLKOWSKI A A,CLASSEN H L.The study of riboflavin requirement in broiler chickens[J].International Journal for Vitamin and Nutrition Research,1998,68(5):316-327.

[2] MANTHEY K C,RODRIGUEZ-MELENDEZ R,HOI J T,et al.Riboflavin deficiency causes protein and DNA damage in HepG2 cells,triggering arrest in G1 phase of the cell cycle[J].Journal of Nutritional Biochemistry,2006,17(4):250-256.  

[3] 王艳辉,王安,谢富.维生素B2对笼养蛋雏鸭生长性能、内分泌及抗氧化能力的影响[J].动物营养学报,2009,21(1):31-35.

[4] 汪张贵,闫利萍,王志跃,等.日粮添加核黄素对28日龄新扬州雏鸡铁吸收和储存能力的影响[J].中国饲料,2009,16:17-18.

[5] NICHOALDS G E,LAWRENCE J D,SAUBERLICH H E.Assessment of status of riboflavin nutriture by assay of erythrocyte glutathione reductase activity[J].Clinical Chemistry,1974,20(5):624-628.

[6] WISLON A D,HAVERSON K,BLAND P W.Expression of class Ⅱ MHC antigens on normal pig intestinal endothelium[J].Immunology,1986,88:98-103.

[7] JIANG Z Y,JIANG S Q,LIN Y C,et al.Effects of soybean isoflavone on growth performance,meat quality,and antioxidation in male broilers[J].Poultry Science,2007,86:1356-1362.

[8] 王丹莉,张敏红,文杰,等.日粮核黄素水平对热应激条件下肉仔鸡生产性能及免疫机能的影响[J].动物营养学报,2003,15(3/4):19-27.

[9] RIVAS A,FABRICANT J.Indications of immunodepression in chickens infected with various strain of Marek’s disease virus[J].Avian Disease,1988,32:1-8.

[10] 张建海,原广华,庞全海,等.不同核黄素水平对肉仔鸡外周血细胞、免疫器官及生产性能的影响[J].山东家禽,2003(2):9-11.

[11] 王志跃,汪张贵,龚道清,等.核黄素对家禽免疫器官发育的影响[J].中国畜牧杂志,2005,41(10):28-30.

[12] DEYHIM F,TEETER R G.Dietary vitamin and/or trace mineral premix effects on performance humoral mediated immunity and carcass composition of during thermoneutral and high ambient temperature distress[J].Journal of Applied Poultry Research,1993(4):347-355.

[13] 顾景范,陈玉珍,王宗印.全血谷胱甘肽还原酶活性系数评价实验性核黄素缺乏的研究[J].营养学报,1981(3):251-260.

[14] SAUBERLICH H E,DOWDY R P,SKALA J M.Laboratory tests for the assessment of nutritional status[J].Critical Reviews in Clinical Laboratory Sciences,1973(4):215-340.

[15] HUSTAD S,MCKINLEY M C,MCNULTY H,et al.Riboflavin,flavin mononucleotide,and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation[J].Clinical Chemistry,2002,48(9):1571-1577.

[16] LEVIN G,COQAN U,LEVY Y,et al.Riboflavin deficiency and the function and fluidity of rat erythrocyte membranes[J].The Journal of Nutrition,1990,120(8):857-861.

[17] 梁惠芳,柳启沛,徐京.核黄素对大鼠脂质过氧化影响的研究[J].卫生研究,1999,28(6):12-15.

[18] 刘秀玲,王俐,姜春花,等.硫胺素和核黄素对H2O2诱导DNA氧化损伤的影响[J].上海交通大学学报,2009,29(9):1049-1052.

[19] ALTEMUELLER U.The role of vitamins in pork products[J].International Pig Topics,2009,123(7):11-13.

[20] 李绍玉,魏凤仙,郝国庆,等.维生素E与核黄素对肉鸡生产性能及肉质的影响[J].河南农业科学,2005(6):82-84.

[21] DELANE A A,POWERS I T.The influence of riboflavin deficiency on absorption and liver storage of iron in the growth rat[J].British Journal of Nutrition,1986,56:171-179.

[22] 周瑞华,唐仪,杨连秀.维生素B2营养状况对机体铁代谢的影响[J].华北煤炭医学院学报,1999,1(4):295-296.

[23] 陈金文.肉用仔鸡核黄素需求量的研究[J].营养学报,1990,12(4):349-354.
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