动物营养学报 >

2012 , Vol. 24 >Issue 4: 704 - 711

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.04.015

分子营养

断奶仔猪小肠黏膜脂肪酸结合蛋白和二肽转运载体1 mRNA表达发育性变化及谷氨酰胺对其的影响

  • 周琳 ,
  • 曹广添 ,
  • 张帅 ,
  • 杨彩梅 ,
  • 陈安国 ,
  • 洪奇华
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  • 浙江大学动物科学学院, 杭州 310058

收稿日期: 2011-10-17

  网络出版日期: 2012-03-30

基金资助

国家自然科学基金(30700578)

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Intestinal Fatty Acid Binding Protein and Dipeptide Transporter 1 mRNA in the Small Intestinal Mucosa of Weaner Piglets: Developmental Expression and Influence of Glutamine

  • ZHOU Lin ,
  • CAO Guangtian ,
  • ZHANG Shuai ,
  • YANG Caimei ,
  • CHEN Anguo ,
  • HONG Qihua
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  • College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2011-10-17

  Online published: 2012-03-30

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摘要

本试验旨在研究断奶仔猪小肠黏膜脂肪酸结合蛋白(I-FABP)和二肽转运载体1(PEPT1)mRNA表达的发育性变化及谷氨酰胺对其的影响。以69头(21±3)日龄断奶杜×长×大仔猪为试验动物,断奶当天选取3头猪进行屠宰,剩余66头随机分为2组,每组3个重复,每个重复11头。对照组饲喂基础饲粮,试验组饲喂基础饲粮+1%谷氨酰胺。断奶后第3、5、7、14天试验组和对照组分别选取3头猪进行屠宰(共计27头),取十二指肠、空肠和回肠黏膜组织样品,通过实时定量PCR法测定I-FABP和PEPT1 mRNA的表达量。结果表明:1)I-FABP和PEPT1 mRNA的表达量各肠段间无显著差异(P>0.05);2)I-FABP和PEPT1 mRNA在十二指肠、空肠和回肠的表达量均在断奶后急剧下降,断奶第3天的表达量最低,显著低于断奶当天(P<0.05),而后逐渐升高,第14天达到峰值;3)试验组I-FABP和PEPT1 mRNA表达量与对照组无显著差异(P>0.05),但试验组表现出促使十二指肠、空肠、回肠黏膜的I-FABP和十二指肠PEPT1 mRNA表达提前恢复至断奶前水平的趋势。结果提示,断奶仔猪I-FABP和PEPT1 mRNA表达量随时间而变化,谷氨酰胺对断奶后I-FABP和PEPT1 mRNA表达量的恢复有一定的促进作用。

关键词： 断奶仔猪; 小肠黏膜; 吸收; 小肠脂肪酸结合蛋白; 二肽转运蛋白1; 谷氨酰胺

本文引用格式

周琳 , 曹广添 , 张帅 , 杨彩梅 , 陈安国 , 洪奇华 . 断奶仔猪小肠黏膜脂肪酸结合蛋白和二肽转运载体1 mRNA表达发育性变化及谷氨酰胺对其的影响[J]. 动物营养学报, 2012 , 24(4) : 704 -711 . DOI: 10.3969/j.issn.1006-267x.2012.04.015

Abstract

This study was conducted to investigate the developmental expression of intestinal fatty acid binding protein (I-FABP) and dipeptide transporter 1 (PEPT1) mRNA in the small intestinal mucosa of weaner piglets and the influence of glutamine (Gln). Sixty nine crossed piglets (Duroc譒andrace譒arge white) aged (21?3) d were used as the exprimental animals, 3 piglets were slaughted at the day of weaning, and the rest 66 piglets were divided into two gruops with 3 replicates in each group and 11 piglets per relicate. Piglets in the control group and the experimental group were fed a basal diet and the basal diet+1% Gln, respectively. Three piglets were slaughtered at 3, 5, 7 and 14 d after weaning, respectively. Mucosal tissues were collected from the duodenum, jejunum and ileum. Real-time PCR was applied to determine the mRNA expressions of I-FABP and PEPT1. The results showed as follows: 1) no significant difference was observed in the mRNA expression levels of I-FABP and PEPT1 in the duodenum, jejunum and ileum (P<0.05); 2) the mRNA expression levels of I-FABP and PEPT1 in the duodenum, jejunum and ileum were decreased obviously due to the weaning, and the expression levels of piglets at the 3rd day postweaning reached the lowest and were significantly lower than those at the wearning day (P<0.05), then the expression levels were increased and reached a peak at the 14th day postweaning; 3) there was no significant difference in mRNA expression levels of I-FABP and PEPT1 between the control group and the experimental group (P>0.05), however, the experimental group showed a trend that I-FABP mRNA expression level in the duodemum, jejunum and ileum as well as PEPT1 mRNA expression level in the duodemum recovered to normal levels early. The results indicate that the mRNA expression levels of I-FABP and PEPT1 in the duodenum, jejunum and ileum of weaner piglets change over time, and Gln plays a role in promoting the recovery of I-FABP and PEPT1 mRNA expression levels of weaner piglets.

Key words： weaner piglet; small intestinal mucosa; absorption; I-FABP; PEPT1; glutamine

参考文献

[1] ZIMMERMAN A W,VAN MOERKERK H T,VEERKAMP J H.Ligand specificity and conformational stability of human fatty acid-binding proteins[J].The International Journal of Biochemistry & Cell Biology,2001,33(9):865-876.  

[2] 刘忠臣,陈代文,余冰,等.不同脂肪来源对断奶仔猪生长性能和脂类代谢的影响[J].动物营养学报,2011,23(9):1466-1474.

[3] HO S Y,STORCH J.Common mechanisms of monoacylglycerol and fatty acid uptake by human intestinal in Caco-2 cells[J].American Journal of Physiology-Cell Physiology,2001,281(4):C1106-C1117.

[4] 初丽丽,王启贵,关天竹,等.I-FABP基因侧翼区多态性与鸡生长和胴体组成性状的相关研究[J].东北农业大学学报,2008,39(9):70-74.

[5] SWEETSER D A,BIRKENMEIER E H,KLISAK I J,et al.The human and rodent intestinal fatty acid binding protein genes.A comparative analysis of their structure,expression,and linkage relation-ships[J].Journal of Biological Chemistry,1987,262(33):16060-16071.

[6] MILOVIC V,TURCHANOWA L,STEIN J,et al.Transepithelial transport of putrescine across monolayers of the human intestinal epithelial cell line,Caco-2[J].World Journal of Gastroenterology,2001,7(2):193-197.

[7] CHARRIER L,MERLIN D.The oligopeptide transporter hPepT1:gateway to the innate immune response[J].Laboratory Investigation,2006,86(6):538-546.

[8] WU G Y,KNABLE D A.Free and protein bound amino acids in sow’s colostrums and milk[J].The Journal of Nutrition,1994,124(3):415-424.

[9] SALLOUM R M,SOUBA W W,KLIMBERG V S,et al.Glutamine is superior to glutamate in supporting gut metabolism,stimulating instestinal glutaminase activity and preventing bacterial translocation[J].Surg Forum,1989,40:6-8.

[10] 刘涛,彭健,周诗其,等.外源性谷氨酰胺和谷氨酸对早期断奶仔猪肠粘膜形态、结构和小肠吸收功能及骨骼肌中DNA、RNA浓度的影响[J].中国兽医学报,2003,23(1):62-65.

[11] MONTOUDIS A,DELVIN E,MENARD D,et al.Intestinal-fatty acid binding protein and lipid transport in human intestinal epithelial cells[J].Biochemical and Biophysical Research Communications,2006,339(1):248-254.  

[12] MONTOUDIS A,SEIDMAN E,BOUDREAU F,et al.Intestinal fatty acid binding protein regulates mitochondrion β-oxidation and cholesterol uptake[J].The Journal of Lipid Research,2008,49:961-972.

[13] VEERKAMP J H,MAATMAN R G.Cytoplasmic fatty acid-binding proteins:their structure and genes[J].Progress in Lipid Research,1995,34(1):17-52.  

[14] 姜延志,李学伟.猪I-FABP基因的分子克隆与组织特异性表达分析[J].遗传学报,2006,33(2):125-132.

[15] APONTE G W.PYY-mediated fatty acid induced intestinal differentiation[J].Peptides,2002,23(2):367-376.  

[16] 常晓彤,侯丽娟,王振辉,等.IFABP基因单核昔酸多态性与2型糖尿病患者血清脂质水平的关系[J].中国糖尿病杂志,2007,15(5):285-288.

[17] ADIBI S A.Regulation of expression of the intestinal oligopeptide transporter (Pept-1) in health and disease[J].American Journal of Physiology and Gastrointestine,2003,285(5):779-788.

[18] OGIHARA H,SUZUKI T,NAGAMACHI Y,et al.Peptide transporter in the rat small intestine:ultrastructural localization and the effect of starvation and administration of amino acids[J].The Histochemical Journal,1999,31(3):169-174.  

[19] TANAKA H,MIYAMOTO K I,MORITA K,et al.Regulation of the PepT1 peptide transporter in the rat small intestine in response to 5-fluorouracil-induced injury[J].Gastroenterology,1998,114(4):714-723.  

[20] CHEN H,WONG E A,WEBB K E,Jr.Tissue distribution of a peptide transporter mRNA in sheep,dairy cows,pigs,and chickens[J].Journal of Animal Science,1999,77(5):1277-1283.

[21] 石常友,宾石玉,褚武英,等.藏猪肠道不同部位CAT1、EAAC1和Pept1 mRNA的特异性分布[J].广西师范大学学报:自然科学版,2009,27(3):71-75.

[22] 陈敦学,石常友,宾石玉,等.哺乳藏猪肠道CAT1、EAAC1和Peptl mRNA的发育性表达分析[J].广西师范大学学报:自然科学版,2010,28(1):63-67.

[23] FREEMAN T C,BENTSEN B S,THWESAIT D T,et al.H+/di-tripeptide transporter (PepT1) expression in the rabbit intestine[J].Pflugers Archiv European Journal of Physiology,1995,430(3):390-400.

[24] 邹仕庚,冯定远,黄志毅,等.猪肠道寡肽转运载体1(PepT1)mRNA表达的肠段特异性和发育性变化[J].农业生物技术学报,2009,17(2):229-236.

[25] MIYAMOTO K I,SHIRAGA T,MORITA K,et al.Sequence,tissue distribution and developmental changes in rat intestinal oligopeptide transporter[J].Biochemica et Biophysica Acta(BBA)-Gene Structure and expression,1996,1305(1/2):34-38.

[26] 杨彩梅,徐卫丹,陈安国.甘氨酰-L-谷氨酰胺对断奶仔猪性能和消化道吸收功能的影响[J].中国畜牧杂志,2005,41(8):6-7.

[27] 赵玉蓉,王红权,贺建华,等.谷氨酰胺对断奶仔猪抗菌肽PR-39 mRNA的表达调控[J].动物营养学报,2009,21(4):567-572.
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