本试验旨在探讨饲粮中添加不同氨基酸组成的二肽对羊体外瘤胃发酵特性的影响。以4只装有永久性瘤胃瘘管的健康南江黄羊作为瘤胃液供体,采用单因素试验设计,共5个处理,对照组采用基础培养底物,试验组在基础培养底物中分别添加2%的亮氨酸-色氨酸(Leu-Trp)、缬氨酸-蛋氨酸(Val-Met)、酪氨酸-丙氨酸(Tyr-Ala)和苯丙氨酸-缬氨酸(Phe-Val)。培养后2、4、8、12、24 h,测定培养液pH、氨态氮(NH3-N)浓度、挥发性脂肪酸(VFA)含量以及微生物蛋白质(MCP)产量。结果表明:各时间点Val-Met组培养液pH低于其他各组,除24 h外其他时间点均差异显著(P<0.05);与对照组相比,试验组总挥发性脂肪酸(TVFA)浓度和MCP产量均显著提高(P<0.05),NH3-N浓度以及乙酸/丙酸均显著降低(P<0.05);Val-Met组TVFA浓度和MCP产量显著高于其他试验组(P<0.05),NH3-N浓度在一定程度上(P>0.05)或显著(P<0.05)低于其他试验组,另外2、4和12 h时Val-Met组的乙酸/丙酸也显著低于其他试验组(P<0.05)。结果提示,饲粮中添加二肽有利于瘤胃微生物的发酵,提高VFA含量和MCP产量,降低NH3-N浓度以及乙酸/丙酸,本试验条件下,以添加2% Val-Met效果最好。
This experiment was conducted to explore the effects of supplementation of di-peptides consisting of different amino acids in diet on in vitro fermentation characteristics of goats. Four healthy Nanjiang brown goats with permanent rumen fistula were selected as donating animals for rumen fluid, and a single-factor design was applied. There were five treatments, the basal culture medium was used in the control group, and the basal culture mediums supplemented with different di-peptides (Leu-Trp, Val-Met, Tyr-Ala and Phe-Val) at 2% were used in experimental groups. After cultured for 2, 4, 8, 12 and 24 h, rumen fluid pH, ammonia nitrogen (NH3-N) concentration, volatile fatty acids (VFA) contents and microbial protein (MCP) yield were determined. The results showed as follows: pH of Val-Met group was significantly lower than that of the other groups at every incubation time point (P<0.05); total VFA concentration and MCP yield of experimental groups were significantly higher than those of the control group (P<0.05), while NH3-N concentration and acetate/propionate were significantly lower than those of the control group (P<0.05); total VFA concentration and MCP yield of Val-Met group were significantly higher than those of the other experimental groups (P<0.05), while NH3-N concentration (P>0.05 or P<0.05) and acetate/propionate (2, 4 and 12 h, P<0.05) were lower than those of the other experimental groups. The results indicate that supplementation of di-peptides in diet can promote fermentation of rumen microbes, increase VFA content and MCP yield, but decrease NH3-N concentration and acetate/propionate; under conditions in this experiment, Val-Met is proved to be more effective.
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