本试验旨在研究饲粮铜添加水平对育成期水貂生长性能、营养物质消化率及氮代谢的影响。选择(60±3)日龄健康雄性水貂80只,随机分成8组,每组10个重复,每个重复1只貂。8组水貂分别饲喂铜添加水平为0(Ⅰ组)、4(Ⅱ组)、8(Ⅲ组)、16(Ⅳ组)、32(Ⅴ组)、64(Ⅵ组)、128(Ⅶ组)、256 mg/kg(Ⅷ组)的试验饲粮。预试期7 d,正试期60 d。结果表明:Ⅴ组的末重和平均日增重极显著高于Ⅷ组(P<0.01),而料重比则极显著低于Ⅷ组(P<0.01)。Ⅴ组的平均日采食量极显著高于Ⅰ和Ⅶ组(P<0.01),显著高于Ⅲ组(P<0.05)。Ⅴ组的干物质采食量极显著高于Ⅷ组(P<0.01)。Ⅴ组的干物质消化率极显著高于Ⅵ组(P<0.01),显著高于Ⅱ和Ⅷ组(P<0.05)。Ⅴ组的蛋白质消化率极显著高于Ⅷ组(P<0.01),显著高于Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅵ和Ⅶ组(P<0.05)。Ⅳ和Ⅴ组的脂肪消化率显著高于Ⅷ组(P<0.05)。Ⅴ组的食入氮极显著高于Ⅷ组(P<0.01)。Ⅴ组的氮沉积极显著高于Ⅱ、Ⅲ、Ⅵ和Ⅷ组(P<0.01),显著高于Ⅰ和Ⅳ组(P<0.05)。Ⅴ组的净蛋白质利用率显著高于Ⅱ、Ⅲ、Ⅵ和Ⅷ组(P<0.05)。Ⅴ组的蛋白质生物学价值极显著高于Ⅲ组(P<0.01),显著高于Ⅰ、Ⅱ、Ⅵ和Ⅷ组(P<0.05)。由此得出,饲粮铜添加水平为32 mg/kg(总铜水平为39 mg/kg)时,营养物质消化率、氮沉积、净蛋白质利用率及蛋白质生物学价值较为理想,且可获得较佳的生长性能。
This experiment was conducted to study the effects of dietary copper supplemental level on growth performance, nutrient digestibility and nitrogen metabolism of growing minks. Eighty healthy male minks at the age of (60±3) days were randomly divided into 8 groups with 10 replicates each and 1 mink per replicate. The minks in the 8 groups were fed experimental diets with copper supplemental levels of 0 (group Ⅰ), 4 (group Ⅱ), 8 (group Ⅲ), 16 (group Ⅳ), 32 (group Ⅴ), 64 (group Ⅵ), 128 (group Ⅶ) and 256 mg/kg (group Ⅷ), respectively. The pre-test period lasted for 7 days and the trial period lasted for 60 days. The results showed as follows: the final weight and average daily gain in the group Ⅴ were extremely significantly higher than those in the group Ⅷ (P<0.01), while the feed/gain in the group Ⅴ was extremely significantly lower than that in the group Ⅷ (P<0.01). The average daily feed intake in the group Ⅴ was extremely significantly higher than that in the groups Ⅰ and Ⅶ (P<0.01), and was significantly higher than that in the group Ⅶ (P<0.05). The dry matter (DM) intake in the group Ⅴ was extremely significantly higher than that in the group Ⅷ (P<0.01). The DM digestibility in the group Ⅴ was extremely significantly higher than that in the group Ⅵ (P<0.01), and was significantly higher than that in the groups Ⅱ and Ⅷ (P<0.05). The protein digestibility in the group Ⅴ was extremely significantly higher than that in the group Ⅷ (P<0.01), and was significantly higher than that in the groups Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅵ and Ⅶ (P<0.05). The fat digestibility in the groups Ⅳ and Ⅴ was significantly higher than that in the group Ⅷ (P<0.05). The nitrogen intake in the group Ⅴ was extremely significantly higher than that in the group Ⅷ (P<0.01). The nitrogen deposition in the group Ⅴ was extremely significantly higher than that in the groups Ⅱ, Ⅲ, Ⅵ and Ⅷ (P<0.01), and was significantly higher than that in the groups Ⅰ and Ⅳ (P<0.05). The net protein utilization in the group Ⅴ was significantly higher than that in the groups Ⅱ, Ⅲ, Ⅵ and Ⅷ (P<0.05). The biological value of protein in the group Ⅴ was extremely significantly higher than that in the group Ⅲ (P<0.01), and was significantly higher than that in the groups Ⅰ, Ⅱ, Ⅵ and Ⅷ (P<0.05). In conclusion, when the dietary copper supplemental level arrives at 32 mg/kg (total copper level is 39 mg/kg), the nutrient digestibility, nitrogen deposition, net protein utilization and biological value of protein of growing minks are more ideal, and the growth performance is better.
[1] UNDERWOOD E J.The mineral nutrition in livestock[M].Aberdeen:The Central Press,1966.
[2] BRAUDE R.Some observations on the need for copper in the diet of fattening pigs[J].The Journal of Agricultural Science,1945,35:163-167.
[3] HAWBAKER J A,SPEER V C,HAYS V W,et al.Effect of copper sulfate and other chemotherapeutics in growing swine rations[J].Journal of Animal Science,1961,20:163-167.
[4] BARBER R S,BRAUDE R,MITCHELL K G.Copper sulphate and molasses distillers dried solubles as dietary supplements for growing pigs[J].Animal Production,1962,4(2):233-238.
[5] LUO X G,DOVE C R.Effect of dietary copper and fat on nutrient utilization, digestive enzyme activities, and tissue mineral levels in weanling pigs[J].Journal of Animal Science,1996,74(8):1888-1896.
[6] FISHER C.Use of copper sulfate as a growth promoter for broiler[J].Feedstuff,1973,16:24-25.
[7] 周文卿.高铜添加剂对肉仔鸡促生长作用的研究 .中国畜牧兽医学会动物营养研究会第二届饲料营养学术讨论会论文摘要汇编. : ,1994.
[8] 龚月生,薛桥,曹雨莉.高剂量铜对肉仔鸡生产性能的影响[J].黄牛杂志,2002,20(1):64-65.
[9] 夏中生,覃小荣,王振权,等.饲粮高剂量铜对肉仔鸡生产性能的影响[J].广西农业生物科学,2000,19(1):39-42.
[10] KING J O L.The feeding of copper sulphate to growing rabbits[J].British Veterinary Journal,1975,131:70-75.
[11] 马德磊.日粮铜水平对生长肉兔生产性能、消化代谢、血液生化指标和MT-Ⅰ mRNA表达量的影响 .硕士学位论文.泰安:山东农业大学,2009.
[12] CASTELL A G,BOWLAND J P.Supplemental copper for swine:growth,digestibility,and carcass measurements[J].Canadian Journal of Animal Science,1968,48:403-411.
[13] DROULISCOS N J,BOWLAND J P,ELLIOT J I.Influence of supplemental dietary copper on copper concentration of pig blood,selected tissues,and digestive tract contents[J].Canadian Journal of Animal Science,1970,50:113-120.
[14] JENKINS N T,MORRIS T R,VALAMOTIS D.The effect of diet and copper supplementation on chick growth[J].British Poultry Science,1970,11:241-248.
[15] AULERICH R J,RINGER R K,BLEAVINS M R,et al.Effects of supplemental dietary copper on growth, reproductive performance and kit survival of standard dark mink and the acute toxicity of copper to mink[J].Journal of Animal Science,1982,55:337-343.
[16] NRC.Nutrient requirements of mink and foxes[S].Washington,D.C.:National Academy Press,1982.
[17] 全国饲料工业标准化技术委员会.GB/T 6435—2006饲料中水分和其他挥发性物质含量的测定[S].北京:中国标准出版社,2006.
[18] 全国饲料工业标准化技术委员会.GB/T 6433—2006饲料中粗脂肪测定方法[S].北京:中国标准出版社,2006.
[19] 全国饲料工业标准化技术委员会.GB/T 6432—1994饲料中粗蛋白测定方法[S].北京:中国标准出版社,2006.
[20] AULERICH R J,RINGER R K.Feeding copper sulfate:could it have benefits in nutrition of mink?[J].American Fur Rancher,1976,56(12):4.
[21] 高秀华,杨福合.饲料蛋白质水平对育成期雄性水貂的影响[J].科技简讯,1989(1):53-54.
[22] 张文丽.粉状高铜饲料对仔猪生长性能的影响[J].饲料研究,2009(4):39-42.
[23] 王凤铃,孙丽芹,董新伟,等.脂类催化氧化[J].中国油脂,1998,23(4):52-54.
[24] 程忠刚,林映才,郑黎.饲料的氧化变质、发热变味[J].饲料博览,2000(11):27-28.
[25] KIRCHGESSNER M,BEYER M G,STEINHART H.Activation of pepsin (EC 3.4.4.1) by heavy metal ions including a contribution to the mode of action of copper sulfate in pig nutrition[J].British Journal of Nutrition,1976,36:15-22.
[26] 程忠刚,许梓荣,林映才.高剂量铜对仔猪生长性能及血液生化指标的影响[J].动物营养学报,2004,16(4):44-46.
[27] 陈杰.家畜生理学[M].北京:中国农业出版社,2006.
[28] PFEIFFER A,HENKEL H,VERSTEGEN M A.The influence of protein intake on water balance,flowrate and apparent digestibility of nutrition at the distal ileum in growing pigs[J].Livestock Product Science,1995,44:179-187.
[29] KERR B J,MCKEITH F K,EASTER R A.Effect on performance and carcass characteristics of nursery to finisher pig s fed reduced crude protein amino acid supplemented diet[J].Journal of Animal Science,1995,73:433-440.
[30] NEWELL C W.Nutrient floe and manure management in the mink industry .Ph.D.Thesis.Halifax,N.S.:Dalhousie University,1999.
[31] BRAUDE R.Copper as a growth stimulant in pigs (cuprum pro pecunia)[M]//Symposium on copper's role in plant and animal life.Vienna:Copper Development Council,1965:55-56.