本试验利用体外产气法研究蛋氨酸羟基类似物异丙酯(HMBi)对瘤胃发酵参数的影响。试验分为3个组,分别为对照组、试验1组和试验2组。其中,2个试验组的培养管中分别加入干物质量1%和2%的HMBi。分别在培养至3、6、9、12、24 h时测定产气量,培养12、24 h后测定发酵液pH以及挥发性脂肪酸和乳酸浓度。结果表明:12 h时2个试验组的乙酸、丁酸、异戊酸和总挥发性脂肪酸浓度均显著高于对照组(P<0.05),但2个试验组间差异不显著(P>0.05);24 h时各挥发性脂肪酸以及总挥发性脂肪酸浓度试验各组间均无显著差异(P>0.05)。试验各组的pH在12、24 h时均处于正常范围,12 h时pH试验1组高于对照组(P>0.05)和试验2组(P<0.05);而24 h时pH试验各组间均无显著差异(P>0.05)。24 h时2个试验组的乳酸浓度均高于对照组,且差异显著(P<0.05),但2个试验组间差异不显著(P>0.05)。3、6、12 h时2个试验组的产气量均高于对照组,且差异显著(P<0.05),但2个试验组间差异不显著(P>0.05)。由此得出,在发酵底物中添加HMBi可改善瘤胃体外发酵。
This study was conducted to investigate the influence of 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMBi) on rumen fermentation parameters in vitro. There were 3 groups (control group, trial groups 1 and 2) in the trial, and culture tubes in trial groups 1 and 2 were added with 1% and 2% HMBi (DM basis), respectively. The gas production of fermentation broth was measured after cultured for 3, 6, 9, 12 and 24 h. The concentrations of volatile fatty acid (VFA) and lactic acid, and pH of fermentation broth were measured after cultured for 12 and 24 h. The results showed that the concentrations of acetate, butyrate, isovalerate and total VFA at 12 h in trial groups were significantly higher than those in the control group (P<0.05), but there were no significant differences between the trial groups (P>0.05). No significant differences were observed in concentrations of proportions of VFA and total VFA at 24 h among all groups (P>0.05). The pH at 12 and 24 h in all groups were in normal range, and pH at 12 h in trial group 1 was higher than that in control group (P>0.05) and trial group 2 (P<0.05), while the pH at 24 h was no significant difference among all groups (P>0.05). The lactic acid concentration at 24 h in trial groups was significantly higher than that in the control group (P<0.05), but there was no significant difference between the trial groups (P>0.05). The gas production at 3, 6 and 12 h in trial groups was higher than that in the control group (P<0.05), but there was no significant difference between the trial groups (P>0.05). In conclusion, the rumen fermentation can be improved by supplementation of HMBi.
[1] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:7-55.
[2] 唐一国,龙瑞军,补小艳,等.反刍家畜饲料营养价值体外法评定研究进展[J].中国奶牛,2002(5):31-33.
[3] NOFTSGER S,ST-PIERRER N R,SYLVESTER J T.Determination of rumen degradability and ruminal effects of three sources of methionine in lactating cows[J].Journal of Dairy Science,2005,88(1):223-237.
[4] ST-PIERRE N R,SYLVESTER J T.Effects of 2-hydroxy-4-(methylthio) butanoic acid (HMB) and its isopropyl ester on milk production and composition by Holstein cows[J].Journal of Dairy Science,2005,88(7):2487-2497.
[5] 郗伟斌,夏科,张永根,等.补充2-羟基-4-甲硫基-丁酸异丙酯对奶牛生产性能及血液生化指标的影响[J].动物营养学报,2010,22(2):372-379.
[6] MENKE K H,RAAB L,SALEWSKI A,et al.The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro[J].Journal of Agricultural Science,1979,93(1):217-222.
[7] 赵广永,冯仰廉.用短期人工瘤胃发酵法研究饮水温对反刍动物瘤胃发酵的影响[J].中国畜牧杂志,1996,32(5):8-10.
[8] 胡伟莲.皂苷瘤胃发酵与甲烷产气量及动物产性能影响的研究 .博士学位论文.杭州:浙江大学,2005:45-48.
[9] BARKER S B,SUMMERSON W H.The colorimetric determination of lactic acid in biological material[J].Journal of Biological Chemistry,1941,138:535-554.
[10] 周为琴,赵健亚,郭玉华,等.不同粗精比底物对体外发酵pH值和发酵产物VFA的影响[J].家畜生态学报,2005(4):50-54.
[11] 韩正康,陈杰.反刍动物瘤胃的消化和代谢[M].北京:科学出版社,1988:64-75.
[12] 刘敏雄.反刍动物消化生理学[M].北京:北京农业大学出版社,1991:91-153.
[13] 龙际飞,赵胜军,刘艳枝,等.利用体外产气法研究2-甲基丁酸对山羊瘤胃发酵的影响[J].中国饲料,2007(8):23-25,28.
[14] 毛胜勇,张耿,朱伟云.延胡索酸二钠对瘤胃微生物体外发酵的影响[J].南京农业大学学报,2006,29(4):80-85.
[15] CALSAMIGLIA S,FERRET A,DEVANT M.Effects of pH and pH fluctuations on microbial fermentation and nutrient flow from a dual-flow continuous culture system[J].Journal of Dairy Science,2002,85(3):574-579.
[16] 郭冬生.反刍动物日粮组合效应对瘤胃发酵和可利用粗蛋白的影响研究 .硕士学位论文.北京:中国农业大学,2004:37-40.
[17] GULIYE A,ATASOGLU C,WALLCE R.Assessment of amino acid requirements for optimum fermentation of xylan by mixed micro-organisms from the sheep rumen[J].Animal Science,2005,80(3):353-360.
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