本试验旨在研究不同外源寡糖对奶山羊粪便微生物区系的影响。试验采用不完全拉丁方设计,选用平均体重为(32.80±2.45) kg、安装有永久性瘤胃瘘管的崂山奶山羊6只,分为6组,试验组羊分别饲喂在基础饲粮中添加1%的甘露寡糖(MOS组)、半乳甘露寡糖(GMOS组)、寡木糖(XOS组)、低聚异麦芽糖(IMO组)和果寡糖(FOS组)的试验饲粮,对照组(CT组)羊饲喂基础饲粮。共进行4期试验,每期35 d,其中预试期14 d,正试期21 d。于正试期第1、7、14、21天晨饲前采集新鲜粪便,测定粪便中乳酸杆菌、双歧杆菌、大肠杆菌和沙门氏菌的数量。结果表明:与CT组相比,试验组乳酸杆菌数量均有所增加,其中FOS组第7天开始达到极显著水平(P<0.01);试验组双歧杆菌数量增加,其中FOS组各采样时间均达到极显著水平(P<0.01);试验组大肠杆菌数量有所降低,其中MOS组和FOS组第7天达到极显著水平(P<0.01)。本试验条件下,不同外源寡糖对增加奶山羊粪便乳酸杆菌、双歧杆菌数量,降低大肠杆菌数量的作用效果不同,其中果寡糖效果较明显。
祁茹
,
温建新
,
程明
,
肖宇
,
褚永康
,
胡静
,
朱亚俊
,
陈俏俏
,
林英庭
. 外源寡糖对奶山羊粪便微生物区系的影响[J]. 动物营养学报, 2012
, 24(6)
: 1165
-1172
.
DOI: 10.3969/j.issn.1006-267x.2012.06.025
The effects of different exogenous oligosaccharides on fecal microflora of dairy goats were studied in this experiment. Six Laoshan dairy goats with an average body weight of (32.80?2.45) kg fitted with permanent rumen cannula were used, and an incomplete Latin square design was applied. Goats in experimental groups were fed a basal diet supplemented with 1% mannose-oligosaccharides (MOS group), galactomannan-oliggosaccharides (GMOS group), xylo-oligosaccharides (XOS group), isomalto-oligosaccharides (IMO group) and fructo-oligosaccharides (FOS group), respectively, and those in the control group (CT group) were fed the basal diet. The experiment consisted of 4 stages with 35 d in each stage, each of which included a 14-day pre-experimental period and a 21-day experimental period. Fresh fecal sample was collected for the analysis of numbers of Lactobacillus, Bifidobacteria, E. coli. and Salmonella on day 1 ,7, 14 and 21 before morning feeding. The results showed as follows: compared with the control group, number of Lactobacillus of experimental groups was increased, and the number of FOS group was significantly increased since day 7 (P<0.01); number of Bifidobacteria was also increased, and the number of FOS group was significantly increased throughout the whole experiment (P<0.01); while number of E. coli were decreased, and the numbers of MOS group and FOS group were significantly increased on day 7 (P<0.01). The results indicate that improvements on fecal Lactobacillus and Bifidobacteria and inhibition on E. coli of different exogenous oligosaccharides are different, and fructo-oligosaccharides are considered to be more efficient.
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