饲粮不同采食水平下肉羊氮沉积和尿中嘌呤衍生物排出规律的研究

马涛; 刁其玉; 邓凯东; 姜成钢; 屠焰; 王永超; 刘洁; 赵一广

doi:10.3969/j.issn.1006-267x.2012.07.007

动物营养学报 >

2012 , Vol. 24 >Issue 7: 1229 - 1235

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.07.007

反刍动物营养

饲粮不同采食水平下肉羊氮沉积和尿中嘌呤衍生物排出规律的研究

马涛 ,
刁其玉 ,
邓凯东 ,
姜成钢 ,
屠焰 ,
王永超 ,
刘洁 ,
赵一广

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1. 中国农业科学院饲料研究所, 北京 100081;
2. 金陵科技学院动物科学与技术学院, 南京 210038

收稿日期: 2012-01-07

网络出版日期: 2012-07-05

基金资助

国家现代农业肉羊产业技术体系专项资金资助(CARS-39);公益性行业科研专项"饲料营养价值与畜牧饲养标准研究与应用"(200903006-03)

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Nitrogen Retention and Urinary Excretion of Purine Derivatives of Mutton Sheep at Different Levels of Feed Intake

MA Tao ,
DIAO Qiyu ,
DENG Kaidong ,
JIANG Chenggang ,
TU Yan ,
WANG Yongchao ,
LIU Jie ,
ZHAO Yiguang

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1. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. College of Animal Science, Jinling Institute of Technology, Nanjing 210038, China

Received date: 2012-01-07

Online published: 2012-07-05

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摘要

本试验旨在研究饲粮不同采食水平下肉羊氮沉积和尿中嘌呤衍生物(PD)的排出规律。试验选用12只平均体重为(41.3±2.8) kg的杜寒杂交绵羊公羔,随机平均分为3组,按照自由采食、自由采食量的70%和自由采食量的40% 3个干物质采食水平饲喂,试验期为12 d,其中预试期7 d,正试期5 d。结果表明:随着饲粮采食水平的降低,营养物质消化率均显著上升(P<0.05),粪氮和尿氮排出量均显著降低(P<0.05),沉积氮与摄入氮之间存在线性相关(R²=0.92)。尿中PD排出量和微生物氮(MN)产量均随采食水平的降低而显著降低(P<0.05),嘌呤氮指数(PNI)与氮沉积率和氮吸收率均表现出相同变化趋势。由此可见,PD排出量与可消化有机物采食量(DOMI)以及MN产量之间均存在线性相关,相关方程分别为Y=21.41X-1.81(R²=0.94)和Y=0.91X+2.57(R²=0.94);PNI能够将氮平衡和PD排出量有机结合起来,用于评价饲粮氮的利用效率。

关键词： 肉羊; 可消化有机物采食量; 尿嘌呤衍生物; 嘌呤氮指数; 微生物氮

本文引用格式

马涛 , 刁其玉 , 邓凯东 , 姜成钢 , 屠焰 , 王永超 , 刘洁 , 赵一广 . 饲粮不同采食水平下肉羊氮沉积和尿中嘌呤衍生物排出规律的研究[J]. 动物营养学报, 2012 , 24(7) : 1229 -1235 . DOI: 10.3969/j.issn.1006-267x.2012.07.007

Abstract

This experiment was aimed to investigate N retention and urinary excretion of purine derivatives (PD) of mutton sheep at different levels of feed intake. Twelve Dorper thin-tailed Han crossbred, non-castrated male lambs with an average bodyweight of (41.3 ? 2.8) kg were randomly assigned to three levels of dry matter intake: ad libitum intake, 70% of the ad libitum intake, and 40% of the ad libitum intake. The experiment lasted for 12 days (7 days for adaptation and 5 days for trial period). The results showed that the nutrient digestibility was significantly increased with decreasing feed intake (P<0.05), whereas both fecal and urinary N excretion were significantly decreased with decreasing feed intake (P<0.05). A linear relationship was found between N retention and N intake (R²=0.92). With the reduction of feed intake, urinary excretion of PD as well as microbial N (MN) yield were significantly decreased (P<0.05). Purine nitrogen index (PNI) showed the same trend as N retention and N absorption. In conclusion, there are linear equations between urinary PD excretion, digestible organic matter intake (DOMI) and also MN yield. The equations are Y=21.41X-1.81 (R²=0.94) and Y=0.91X+2.57 (R²=0.94), respectively. The combination of both N balance and PD excretion can be expressed as PNI, which can be used to evaluate utilization of dietary nitrogen.

Key words： mutton sheep; digestible organic matter intake; urinary purine derivatives; purine nitrogen index; microbial N

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