动物营养学报 >

2012 , Vol. 24 >Issue 7: 1352 - 1360

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.07.021

研究简报

γ-氨基丁酸对断喙雏鸡血清细胞因子含量及脾脏中 Bcl-2和Fas基因mRNA表达量的影响

  • 谢婉莹 ,
  • 侯新燕 ,
  • 闫峰宾 ,
  • 韩瑞丽 ,
  • 孙桂荣 ,
  • 康相涛
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  • 河南农业大学牧医工程学院, 河南省家禽种质资源工程研究中心, 郑州 450002

收稿日期: 2012-02-01

  网络出版日期: 2012-07-05

基金资助

现代农业产业技术体系建设专项资金(CARS-41-K04)及河南省重大科技攻关项目(112101110800)

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γ-Aminobutyric Acid Affects Serum Cytokine Contents and mRNA Expression Levels of Bcl-2 and Fas genes in Spleen of Chicks under Beak Trimming Stress

  • XIE Wanying ,
  • HOU Xinyan ,
  • YAN Fengbin ,
  • HAN Ruili ,
  • SUN Guirong ,
  • KANG Xiangtao
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  • College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, China

Received date: 2012-02-01

  Online published: 2012-07-05

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摘要

本试验旨在研究γ-氨基丁酸(GABA)对断喙应激条件下雏鸡生长性能、血清细胞因子含量及脾脏中细胞凋亡相关基因B细胞淋巴瘤/白血病2(B-cell lympoma/leukemia-2,Bcl-2)和Fas基因mRNA表达量的影响。选取1日龄体重相近的健康固始鸡公雏360只,随机分为5个组,每组6个重复,每个重复12只鸡。其中A、B、C组均为断喙组,饮水中分别添加40、60和80 mg/kg的GABA;D组不断喙,不添加GABA;E组断喙,不添加GABA。结果表明:1)断喙应激导致雏鸡采食量减少、体重下降,添加GABA可以提高采食量和体重;2)断喙应激引起了血清白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)含量的升高,第1、5天E组血清IL-1β含量显著高于D组(P<0.05),添加80 mg/kg GABA显著降低了血清IL-1β含量(P<0.05);与D组相比,断喙后第1、7天E组血清TNF-α和IL-6含量均显著升高(P<0.05),添加80 mg/kg GABA后显著降低(P<0.05);3)E组第1、3、7天脾脏中促凋亡基因Fas mRNA表达量显著高于D组,添加GABA后下降,且随着GABA剂量加大而进一步降低(P<0.05),添加80 mg/kg GABA可轻度上调抗凋亡基因Bcl-2 mRNA表达量(P>0.05)。由此可知,GABA在改善断喙应激雏鸡生长性能方面具有良好效果,同时添加GABA抑制了断喙应激后雏鸡血清细胞因子含量以及脾脏中促凋亡基因Fas的mRNA表达量的升高,且添加80 mg/kg效果较好。

关键词： γ-氨基丁酸; 断喙应激; 细胞因子; 细胞凋亡; 雏鸡

本文引用格式

谢婉莹 , 侯新燕 , 闫峰宾 , 韩瑞丽 , 孙桂荣 , 康相涛 . γ-氨基丁酸对断喙雏鸡血清细胞因子含量及脾脏中 Bcl-2和Fas基因mRNA表达量的影响[J]. 动物营养学报, 2012 , 24(7) : 1352 -1360 . DOI: 10.3969/j.issn.1006-267x.2012.07.021

Abstract

This experiment was conducted to investigate the effects of γ-aminobutyric acid (GABA) on growth performance, serum cytokine contents and mRNA expression levels of apoptosis-related gene B-cell lympoma/leukemia-2 (Bcl-2) and Fas gene in spleen of chicks under beak trimming stress. A total of 360 one-day-old healthy Gu-shi male chicks were randomly divided into 5 groups (A, B, C, D and E) with 6 replicates per group and 12 chicks per replicate. Groups A, B and C were supplemented with 40, 60 and 80 mg/kg GABA in drinking water, group D was not supplemented with GABA, and group E was supplemented with GABA. The results showed as follows: 1) beak trimming induced FI and BW to decrease, whereas supplemented with GABA could increased FI and BW of chicks under beak trimming stress (P<0.05). 2) The contents of interleukin-1β (IL-1β), interleukin-6 (IL-6)and tumor necrosis factor-α (TNF-α)in serum were increased by beak trimming stress, the serum IL-1β content in group E was significantly higher than that in group D on the first and fifth days (P<0.05), and the supplementation of 80 mg/kg GABA significantly decreased the serum IL-1β content. Compared with group D, the contents of TNF-α and IL-6 in serum in group E were significantly increased on the first and seventh days (P<0.05), and supplementation of 80 mg/kg GABA significantly decreased the contents of TNF-α and IL-6 in serum (P<0.05). 3) The splenetic Fas gene mRNA expression level in group E was significantly higher than that in group D on the first, third and seventh days (P<0.05), and the splenetic Fas gene mRNA expression level was decreased with GABA supplementation (P<0.05), the supplementation of 80 mg/kg GABA could increase the splenetic Bcl-2 gene mRNA expression level (P>0.05). In conclusion, GABA can improve the growth performance of chick under beak trimming stress, and it also inhibit serum cytokines contents and splenetic Fas gene mRNA expression under beak trimming stress, and the optimum supplemention level of GABA is 80 mg/kg.

Key words： γ-aminobutyric acid; beak trimming stress; cytokine; apoptosis; chicks

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