妊娠后期母猪和仔猪补饲外源精胺对初生和28日龄仔猪肠道形态结构及二糖酶活性的影响

何余湧; 邹伟; 刘晓兰; 王仁华; 陆伟

doi:10.3969/j.issn.1006-267x.2012.08.006

动物营养学报 >

2012 , Vol. 24 >Issue 8: 1429 - 1437

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.08.006

猪与禽营养 Swine and Poultry Nutrition

妊娠后期母猪和仔猪补饲外源精胺对初生和28日龄仔猪肠道形态结构及二糖酶活性的影响

何余湧 ,
邹伟 ,
刘晓兰 ,
王仁华 ,
陆伟

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江西农业大学动物科技学院,南昌 330045

收稿日期: 2012-06-05

网络出版日期: 2012-08-03

基金资助

江西省自然科学基金项目(2008GZN0018)

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Effects of Dietary Exogenous Spermine Supplemented to Late-Pregnant Sows and Suckling Piglets on Intestinal Morphology and Disaccharidase Activity of Newborn and 28-Day-Old Piglets

HE Yuyong ,
ZOU Wei ,
LIU Xiaolan ,
WANG Renhua ,
LU Wei

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College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2012-06-05

Online published: 2012-08-03

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摘要

本文通过研究妊娠后期母猪和仔猪补饲外源精胺对初生和28日龄仔猪肠道形态结构和二糖酶活性的影响,初步探讨干预"仔猪早期断奶综合征"的技术措施。试验第1阶段,选择6头体重和膘情相近、胎次为3、已怀孕91 d的健康"长×大"母猪,随机分为Ⅰ、Ⅱ和Ⅲ 3个组,每个组设2个重复,每个重复1头母猪。Ⅰ、Ⅱ和Ⅲ组妊娠母猪饲粮中外源精胺的添加量为0、1.5和3.0 mg/kg,饲喂至分娩结束;第2阶段,分娩后母猪采食同一种不含外源精胺的饲粮,Ⅰ、Ⅱ和Ⅲ组哺乳仔猪于7日龄起相应补饲外源精胺添加量为0、3.0和6.0 mg/kg的哺乳仔猪饲粮至28日龄。在仔猪初生和28日龄时,分别从每窝仔猪中选1头接近平均体重的健康仔猪进行屠宰,用于胃肠发育指标的测定。结果表明:与未添加外源精胺相比,妊娠母猪饲粮添加3.0 mg/kg外源精胺显著提高了初生仔猪十二指肠和回肠的柱状细胞数量、十二指肠和空肠的杯状细胞数量(P<0.05),显著增加了空肠隐窝深度(P<0.05),显著提高了麦芽糖酶和蔗糖酶比活力(P<0.05);哺乳仔猪饲粮添加6.0 mg/kg外源精胺显著增加了28日龄仔猪空肠黏膜重(P<0.05),显著提高了十二指肠和空肠的麦芽糖酶和蔗糖酶比活力(P<0.05),极显著增加了空肠柱状细胞和杯状细胞数量(P<0.01),极显著增加了十二指肠和空肠隐窝深度(P<0.01)。因此,在妊娠后期母猪和哺乳仔猪饲粮中添加外源精胺有利于初生和28日龄仔猪肠道形态结构的改善和二糖酶活性的提高。

关键词： 外源精胺; 肠道形态结构; 二糖酶; 初生仔猪; 28日龄仔猪

本文引用格式

何余湧 , 邹伟 , 刘晓兰 , 王仁华 , 陆伟 . 妊娠后期母猪和仔猪补饲外源精胺对初生和28日龄仔猪肠道形态结构及二糖酶活性的影响[J]. 动物营养学报, 2012 , 24(8) : 1429 -1437 . DOI: 10.3969/j.issn.1006-267x.2012.08.006

Abstract

A successive feeding experiment was conducted to evaluate the effects of dietary exogenous spermine supplemented to late-pregnant sows and suckling piglets on intestinal morphology and disaccharidase activity of newborn and 28-day-old piglets in order to achieve a technology to alleviate early weaned stress syndrome of piglets. In the stage 1, spermine was added to a late-pregnant sow diet at a level of 0 (group Ⅰ), 1.5 (group Ⅱ) or 3.0 mg/kg (group Ⅲ), respectively, and then the diets were offered randomly to one of three groups of sows (Large White×Yorkshire) from day 91 after pregnancy to parturition, each group had 2 sows, and all the pregnant sows had the similar live weight, condition and the same boar when the experiment started; in the stage 2, suckling piglets in groups Ⅰ, Ⅱ and Ⅲ were accordingly offered a creep diet supplemented with spermine at a level of 0, 3.0 or 6.0 mg/kg from 7 to 28 days of age. At birth and 28 days of age, one piglet with the average body weight from each sow was killed to determine the development of gastrointestinal tract. The results showed that compared with the no-added-spermine diet (group Ⅰ), the pregnant sow diet that added spermine at a level of 3.0 mg/kg significantly increased the number of cylindric cells in duodenum and ileum, the number of goblet cells in duodenum and jejunum, the crypt depth, and the specific activities of maltase and sucrase in jejunum of newborn piglets (P<0.05). The suckling piglet diet that supplemented with the spermine at a level of 6.0 mg/kg significantly increased the mucosal weight of jejunum and the specific activities of maltase and sucrase in duodenum and jejunum (P<0.05), and increased the number of cylindric and goblet cells in jejunum and the crypt depth in duodenum and jejunum of 28-day-old piglets (P<0.01). Spermine supplemented to the diets of late-pregnant sows and of suckling piglets is conducive to the early development of intestinal morphology and disaacharidase activity in newborn and 28-day-old piglets.

Key words： exogenous spermine; intestinal morphology; disaacharidase; newborn piglets; 28-day-old piglets

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