动物营养学报 >

2012 , Vol. 24 >Issue 8: 1583 - 1588

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.08.025

研究简报 Short Communications

甘露寡糖、果寡糖和大豆寡糖组合对锦江黄牛瘤胃液细菌多样性的影响

  • 刘立恒 ,
  • 闵力 ,
  • 瞿明仁 ,
  • 戈婷婷 ,
  • 钟志勇
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  • 江西农业大学江西省高校动物营养与饲料重点实验室,南昌 330045

收稿日期: 2012-02-27

  网络出版日期: 2012-08-03

基金资助

现代农业产业技术体系项目(CARS-38);国家自然科学基金(30960254);江西省教育厅项目(GJJ10405,2009GJJ10409)

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A Combination of Mannose Oligosaccharides, Fructo-Oligosaccharides and Soybean Oligosaccharides Affects Diversity of Rumen Fluid Bacteria of Jinjiang Cattle

  • LIU Liheng ,
  • MIN Li ,
  • QU Mingren ,
  • GE Tingting ,
  • ZHONG Zhiyong
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  • Key Laboratory of Animal Nutrition and Feed Science of University and College in Jiangxi Province, Jiangxi Agriculture University, Nanchang 330045, China

Received date: 2012-02-27

  Online published: 2012-08-03

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摘要

本试验旨在研究功能性寡糖(甘露寡糖、果寡糖和大豆寡糖)组合对锦江黄牛的瘤胃液细菌多样性的影响。选择3头健康且体重相近的安装有永久性瘤胃瘘管的锦江黄牛,采用自身对照方法,试验分2期进行,第1期:对照期,饲喂基础饲粮,不添加任何寡糖;第2期:添加期,饲喂基础饲粮+功能性寡糖(0.8%甘露寡糖+1.0%果寡糖+0.8%大豆寡糖),每期持续14 d。利用PCR-变性梯度凝胶电泳(DGGE)方法研究分析牛瘤胃液细菌多样性。结果表明:添加功能性寡糖组合后,牛瘤胃液细菌PCR-DGGE图谱条带数明显增加,3头牛增加了5个共同条带。经测序,其中2个是产琥珀酸丝状杆菌属,3个是未鉴定细菌。饲粮中添加功能性寡糖组合可使瘤胃液细菌菌群种类明显增加。结果提示,饲粮添加功能性寡糖组合能够影响瘤胃菌群区系,增强其稳定性。

关键词： 功能性寡糖; 变性梯度凝胶电泳; 瘤胃; 细菌; 多样性

本文引用格式

刘立恒 , 闵力 , 瞿明仁 , 戈婷婷 , 钟志勇 . 甘露寡糖、果寡糖和大豆寡糖组合对锦江黄牛瘤胃液细菌多样性的影响[J]. 动物营养学报, 2012 , 24(8) : 1583 -1588 . DOI: 10.3969/j.issn.1006-267x.2012.08.025

Abstract

This experiment was conducted to study the effects of a combination of functional oligosaccharides (mannose oligosaccharides, fructo-oligosaccharides and soybean oligosaccharides) on diversity of rumen fluid bacteria of Jinjiang cattle. Three healthy Jinjiang cattle with permanent rumen cannulas and similar body weight were selected. By self-contrast method, the experiment was divided into two phases, the first phase: the control period, cattle were fed a basal diet without oligosaccharides supplementation; the second period, supplemental period, cattle were fed the basal diet supplemented with 0.8% mannose oligosaccharides, 1% fructo-oligosaccharides and 0.8% soybean oligosaccharides. The experiment lasted for 14 d in each phase. The diversity of rumen fluid bacteria was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE). The results showed as follows: after adding the combination of functional oligosaccharides, the band number of rumen fluid bacteria of Jinjiang cattle on PCR-DGGE map increased obviously. Five common bands were found in the three cattle. Based on the results of sequencing, two bands belonged to Fibrobacter succinogenes, and the other three bands were uncultured bacterium. The results indicate that supplementation of combination of functional oligosaccharides can affect rumen fluid bacterial flora, and increase its stability.

Key words： functional oligosaccharides; denaturing gradient gel electrophoresis; rumen; bacteria; diversity

参考文献

[1] 瞿明仁,凌宝明,卢德勋,等.灌注果寡糖对生长绵羊瘤胃发酵功能的影响[J].畜牧兽医学报,2006,37(8):779-784.

[2] 凌宝明,瞿明仁,卢德勋.体外条件下甘露寡糖对生长绵羊瘤胃发酵功能的影响[J].当代畜牧,2006(6):35-37.

[3] 刘兵,瞿明仁,张学峰,等.瘤胃灌注大豆寡糖对绵羊消化道内营养物质流通与消化的影响[J].畜牧兽医学报,2008,39(7):915-922.

[4] HILL M J R,MARSH P D.Control of large bowel microflora in human microbiology ecology[M].Boca Raton:CRC Press Inc.,1990:95-100.

[5] 吴晓盼.利用PCR-DGGE技术研究益生菌和抗生素对肉鸡肠道菌群多样性的影响 .硕士学位论文.南昌:江西农业大学,2008.

[6] 张洁.不同碳源对山羊瘤胃发酵和微生物氨基酸组成影响的研究 .硕士学位论文.扬州:扬州大学,2008.

[7] WHITFORD M F,FORSTER R J,BEARD C E,et al.Phylogenetic analysis of rumen bacteria by comparative sequence analysis of cloned 16S rRNA genes[J].Anaerobe,1998,4:153-163.

[8] SHARP R,ZIEMER C J,MARSHALL D S,et al.Taxon-specific associations between protozoal and methanogen populations in the rumen and a model system[J].FEMS Microbiology Ecology,1998,26:71-78.

[9] WAND H F,ZHU W Y,YAO W,et al.DGGE and 16S rDNA sequencing analysis of bacterial communities in colon content and feces of pigs fed whole crop rice[J].Anaerobe,2007,13:127-133.

[10] VAN ORSOUW N J,LI D,VIJG J.Denaturing gradient gel electrophoresis (DGGE) increases resolution and information of Alu-directed inter-repeat PCR[J].Molecular and Cellular Probes,1997,11:95-101.

[11] SIMPSON J M,MCCRACKEN V J,WHITE B A,et al.Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota[J].Journal of Microbiological Methods,1999,36:167-179.

[12] DAVIS M E,MAXWELL C V,BROWN D C,et al.Effect of dietary mannan oligosaeeharides and (or) pharmacological additions of copper sulfate on growth performance and immuno-competence of weanling and growing/finishing pigs[J].Journal of Animal Science,2002,80(11):2887-2893.

[13] DONOVAN D C,FRANKLIN S T,CHASE C C L,et al.Growth and health of holstein calves fed milk replacers supplemented with antibiotics or enteroguard[J].Journal of Dairy Science,2002,85(4):947-950.  

[14] 赵晓静,李建国,李秋风,等.甘露寡糖对犊牛粪便菌群影响的研究[J].中国畜牧杂志,2007,43(5):31-34.

[15] 戈婷婷.不同组合的功能性寡糖(NDOs)对锦江黄牛体外发酵的影响 .硕士学位论文.南昌:江西农业大学,2011.

[16] 姚文,朱伟云,韩正康.应用变性梯度凝胶电泳和16S rDNA序列分析对山羊瘤胃细菌多样性的研究[J].中国农业科学,2004,37(9):1374-1378.

[17] KOCHERGINSKAYA S A,AMINOV R I,WHITE B A.Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis,random sequencing,and statistically ecology approaches[J].Anaerobe,2001,7:119-134.

[18] 郭勇庆.牛蒡果寡糖对绵羊生产性能、营养物质消化代谢影响研究 .硕士学位论文.保定:河北农业大学,2010.

[19] 毛胜勇.延胡索酸及其钠盐对瘤胃发酵特性及瘤胃细菌菌群的影响 .博士学位论文.南京:南京农业大学,2006.

[20] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.

[21] JUN H S,QI M,HA1 J K,et al.Fibrobacter succinogenes,a dominant fibrolytic ruminal bacterium:transitionto thepost genomicera[J].Asian-Australasian Journal of Animal Sciences,2007,20:802-810.

[22] 毛华明,马松成,陈静,等.大额牛瘤胃细菌16S rRNA基因序列分析[J].中国农业科学,2008,41(11):3769-3775.

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