动物营养学报 >

2012 , Vol. 24 >Issue 8: 1589 - 1601

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.08.026

研究简报 Short Communications

温度与饲料蛋白质水平对吉富品系尼罗罗非鱼 (Oreochromis nilotica)幼鱼生长和血清生长激素水平的影响

  • 强俊 ,
  • 杨弘 ,
  • 王辉 ,
  • 徐跑 ,
  • 乐贻荣 ,
  • 何杰
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  • 1. 南京农业大学无锡渔业学院,无锡 214081;
    2. 中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室,无锡 214081;
    3. 广东海洋大学水产学院,湛江 524025

收稿日期: 2012-02-10

  网络出版日期: 2012-08-03

基金资助

公益性行业(农业)科研专项(200903046-02);现代农业产业技术体系建设专项资金"罗非鱼产业技术体系"(CARS-49);江苏省普通高校研究生科研创新计划(CXLX11-0708);中央公益性科研业务费专项资金(2011jbfa09);广东省海洋渔业科技推广专项[A201009C02、A2010002-010(b)];广东省科技计划项目(2010B090500032)

收起

Temperature and Dietary Protein Level Affect Growth and Serum Growth Hormone Level in GIFT Nile Tilapia Juvenile (Oreochromis nilotica)

  • QIANG Jun ,
  • YANG Hong ,
  • WANG Hui ,
  • XU Pao ,
  • LE Yirong ,
  • HE Jie
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  • 1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;
    2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
    3. Guangdong Ocean University, Zhanjiang 524025, China

Received date: 2012-02-10

  Online published: 2012-08-03

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摘要

采用中心复合试验设计和响应曲面分析方法,在试验室条件下,研究了温度(20~34 ℃)和饲料蛋白质水平(25%~50%)对吉富品系尼罗罗非鱼(Oreochromis nilotica)幼鱼生长、饲料效率和血清生长激素(GH)水平的影响。试验期为56 d。随着温度和饲料蛋白质水平的上升,特定生长率、饲料效率和血清GH水平均呈先上升后下降的变化趋势。温度与饲料蛋白质水平的一次效应对特定生长率、饲料效率和血清GH水平有显著影响(P<0.05);温度和饲料蛋白质水平的二次效应与互作效应对饲料效率和血清GH水平有显著影响(P<0.05),温度与蛋白质水平对特定生长率无互作效应(P>0.05)。高温与饲料高蛋白质水平会降低饲料效率和血清GH水平。因子与响应值间二次多项回归方程的决定系数分别达到0.958、0.955和0.875(P<0.01),可用于预测。温度/饲料蛋白质水平最优组合为29.9 ℃/40.3%,此时,特定生长率和饲料效率均较高,分别为2.748%/d和77.5%,其可靠性达0.888。温度对特定生长率、饲料效率和血清GH水平影响较饲料蛋白质明显。血清GH水平与特定生长率相关性较低,而与饲料效率呈正相关。因此,建议在罗非鱼幼鱼的培育中,按照上述温度与饲料蛋白质组合安排生产,以提高罗非鱼养殖效益。

关键词： 温度; 饲料蛋白质水平; 生长; 血清GH; 响应曲面

本文引用格式

强俊 , 杨弘 , 王辉 , 徐跑 , 乐贻荣 , 何杰 . 温度与饲料蛋白质水平对吉富品系尼罗罗非鱼 (Oreochromis nilotica)幼鱼生长和血清生长激素水平的影响[J]. 动物营养学报, 2012 , 24(8) : 1589 -1601 . DOI: 10.3969/j.issn.1006-267x.2012.08.026

Abstract

The purpose of this experiment was to examine the effects of temperature (from 20 ℃ to 34 ℃) and dietary protein level (from 25% to 50%) on specific growth rate (SGR), feed efficiency (FE) and serum growth hormone (GH) level in GIFT Nile tilapia juveniles through central composite rotatable experimental design and response surface method under laboratory conditions. The entire experiment lasted for 56 days. Results showed that SGR, FE and serum GH level were increased at the beginning and then decreased with increasing of water temperature and dietary protein level. The linear effects of temperature and dietary protein level on SGR, FE and serum GH level were statistically significant (P<0.05). The quadratic effects of temperature and dietary protein level and reciprocal effects of temperature × dietary protein level on FE and serum GH level were significant (P<0.05); but the reciprocal effects of temperature × dietary protein level on SGR was nonsignificant (P>0.05). High temperature and high dietary protein level inhibited FE and serum GH level. Model equations of the SGR, FE and serum GH level on temperature and dietary protein level were established, with the coefficients of determination being 0.958, 0.955 and 0.875, respectively (P<0.01), and could be applied in prediction. The optimal temperature/dietary protein level combination was obtained utilizing statistical optimization approach: 29.9 ℃/40.3%, at which the maximal SGR and FE reached 2.748%/d and 77.5%, respectively, with the reliability being 0.888. The effects of temperature on SGR, FE and serum GH level were more significant than those of dietary protein level. There was weak correlation between serum GH and SGR. However, a positive correlation was found between serum GH and FE. Thus, breeding efficiency of tilapia juvenile can be improved by rearing the species at an optimal combination of temperature and dietary protein level.

Key words： temperature; dietary protein level; growth; serum GH; response surface

参考文献

[1] AL HAFEDH Y S.Effects of dietary protein on growth and body composition of Nile tilapia[J].Aquaculture Research,1999,30:385-393.

[2] GUNASEKERA R M,LAM T J.Influence of dietary protein level on ovarian recrudescence in Nile tilapia,Oreochromis niloticus (L.)[J].Aquaculture,1997,149:57-69.

[3] EL-SAYED A M,KAWANNA M.Effects of dietary protein and energy levels on spawning performance of Nile tilapia (Oreochromis niloticus) broodstock in a recycling system[J].Aquaculture,2008,258:179-184.

[4] PERES H,OLIVA-TELES A.Influence of temperature on protein utilization in juvenile European seabass (Dicentrarchus labrax)[J].Aquaculture,1999,170:337-348.

[5] PEREZ-VELAZQUEZ M,GONZLEZ-FLIX M L,JAIMES-BUSTAMENTE F,et al.Investigation of the effects of salinity and dietary protein level on growth and survival of Pacific white shrimp,Litopenaeus vannamei[J].Journal of the World Aquaculture Society,2007,38:475-485.

[6] ABDEL-TAWWAB M,AHMAD M H,KHATTAB Y A E,et al.Effect of dietary protein level,initial body weight,and their interaction on the growth,feed utilization,and physiological alterations of Nile tilapia,Oreochromis niloticus (L.)[J].Aquaculture,2010,298:267-274.

[7] OMAR E,AL-SAGHEER F M,NOUR A M,et al.Effect of protein level and stocking density on growth performance,feed utilization and resistance of Nile tilapia (Oreochromis niloticus) to infection against aeromonas septicemia (Aeromonas hydrophila)[J].Cahiers Options Mediterraneennes,1997,22:67-77.

[8] CARMONA-OSALDE C,OLVERA-NOVOA M A,RODRGUEZ-SERNA M.Effect of the protein-lipids ratio on growth and maturation of the crayfish Procambarus (Austrocambarus) llamasi[J].Aquaculture,2005,250:692-699.

[9] PIROZZI I,BOOTH M A,ALLAN G L.The interactive effects of dietary protein and energy on feed intake,growth and protein utilization of juvenile mulloway (Argyrosomus japonicus)[J].Aquaculture Nutrition,2010,16:61-71.

[10] GARCIA J A,VILLARROEL M.Effect of feed type and feeding frequency on macrophage functions in tilapia (Oreochromis niloticus L.)[J].Fish & Shellfish Immunology,2009,27:325-329.

[11] SINGH R K,CHAVAN S L,DESAI A S,et al.Influence of dietary protein levels and water temperature on growth,body composition and nutrient utilization of Cirrhinus mrigala (Hamilton,1822) fry[J].Journal of Thermal Biology,2008,33:20-26.

[12] HIDALGO F,ALLIOT E.Influence of water temperature on protein requirement and protein utilization in juvenile Sea bass,Dicentrarchus labrax[J].Aquaculture,1988,72:115-129.

[13] MUSUKA C G,LIKONGWE J S,KANG’OMBE J,et al.The Effect of dietary protein and water temperatures on performance of T. rendalli juveniles reared in indoor tanks[J].Pakistan Journal of Nutrition,2009,8:1526-1531.

[14] PLANAS J V,MNDEZ E,BAOS N,et al.Insulin and IGF-1 receptors in trout adipose tissue are physiologically regulated by circulating hormone levels[J].The Journal of Experimental Biology,2000,203:1153-1159.

[15] CAMERON C,MOCCIA R,AZEVEDO P A,et al.Effect of diet and ration on the relationship between plasma GH and IGF-1 concentrations in Arctic charr,Salvelinus alpinus (L.)[J].Aquaculture Research,2007,38:877-886.

[16] 姜巨峰,张殿昌,邱丽华,等.用IGF-Ⅰ mRNA表达量评价鲮饲料配方效果的研究[J].南方水产,2010,6:66-72.

[17] IMSLAND A K,BJRNSSON B T,GUNNARSSON S,et al.Temperature and salinity effects on plasma insulin-like growth factor-Ⅰ concentrations and growth in juvenile turbot (Scophthalmus maximus)[J].Aquaculture,2007,271:546-552.

[18] GABILLARD J C,WEIL C,RESCAN P Y,et al.Environmental temperature increases plasma GH levels independently of nutritional status in rainbow trout (Oncorhynchus mykiss)[J].General and Comparative Endocrinology,2003,133:17-26.

[19] CRUZ E M V,BROWN C L.Influence of the photoperiod on growth rate and insulin-like growth factor-Ⅰgene expression in Nile tilapia Oreochromis niloticus[J].Journal of Fish Biology,2009,75:130-141.

[20] BECKMAN B R,LARSEN D A,MORIYAMA S,et al.Insulin-like growth factor-Ⅰ and environmental modulation of growth during smoltification of spring Chinook salmon (Oncorhynchus tshawytscha)[J].General and Comparative Endocrinology,1998,109:325-335.

[21] AYSON F G,TAGAWA M,KANEKO T,et al.Homologous radioimmunoassays for tilapia prolactins and growth hormone[J].General and Comparative Endocrinology,1993,89:138-148.

[22] DENIZ B,BOYACI I H.Modeling and optimization Ⅰ:usability of response surface methodology[J].Journal of Food Engineering,2007,78:836-845.

[23] 胡国成,李思发,何学军,等.不同饲料蛋白质水平对吉富品系尼罗罗非鱼幼鱼生长和鱼体组成的影响[J].饲料工业,2006,27:24-27.

[24] JUANCEY K.The effects of varying dietary protein level on the growth,food conversion,protein utilization and body composition of juvenile tilapias (Sarotherodon mossambicus)[J].Aquaculture,1982,27:43-54.

[25] POPMA T J,LOVSHIN L L.Worldwide prospects for commercial production of tilapia. International center for aquaculture and aquatic environments department of fisheries and allied aquacultures[M].Alabama:Auburn University,1995:8-9.

[26] 钱曦,王桂芹,周洪琪,等.饲料蛋白水平及豆粕替代鱼粉比例对翘嘴红鲌消化酶活性的影响[J].动物营养学报,2007,19(2):182-187.

[27] ALVAREZ-GONZALEZ C A,CIVERA-CERECEDO R,ORTIZ-GALINDO J L,et al.Effect of dietary protein level on growth and body composition of juvenile spotted sand bass,Paralabrax maculatofasciatus,fed practical diets[J].Aquaculture,2001,194:151-159.

[28] GABILLARD J C,WEIL C,RESCAN P Y,et al.Does the GH/IGF system mediate the effect of water temperature on fish growth?A review[J].Cybium,2005,29:107-117.

[29] RICORDEL M J,SMAIL J,LE BAIL P Y.Application of a recombinant cichlid growth hormone radioimmunoassay to measure native GH in tilapia (Oreochromis niloticus) bred at different temperatures[J].Aquatic Living Resources,1995,8:153-160.

[30] BOEUF G,LE BAIL P Y.Does light have an influence on fish growth?[J].Aquaculture,1999,177:129-152.

[31] BJORNSSON B T,THORARENSEN H,HIRANO T,et al.Photoperiod and temperature affect plasma growth hormone levels,growth,condition factor and hypoosmoregulatory ability of juvenile Atlantic salmon (Salmo salar) during parr-smolt transformation[J].Aquaculture,1989,82:77-91.

[32] 邓利,张伟民,林浩然,等.黑鲷生长激素及其受体的季节变化[J].水产学报,2001,25:203-208.

[33] SHEPHERD B S,RON B,BURCH A,et al.Effects of salinity, dietary level of protein and 17α- methyltestosterone on growth hormone (GH) and prolactin (tPRL177 and tPRL188) levels in the tilapia,Oreochromis mossambicus[J].Fish Physiology and Biochemistry,1997,17:279-288.

[34] SWEETING R M,EALES J G.The effects of starvation and food intake on hepatic thyroxine-monodeid inase activity in the rainbow trout,Oncorhynchu smykiss[J].Canadian Journal of Zoology,1992,70:1516-1525.

[35] 王桂芹,周洪琪,陈建明,等.饲料蛋白对翘嘴鲌生长和内分泌激素的影响[J].水生生物学报,2008,32:544-550.

[36] 冯仁勇,周小秋,林燕.类胰岛素生长因子-Ⅰ对鱼类氮代谢的影响[J].动物营养学报,2006,18:372-377.

[37] GMEZ-REQUENI P,MINGARRO M,CALDUCH-GINER J,et al.Protein growth performance,amino acid utilisation and somatotropic axis responsiveness to fish meal replacement by plant protein sources in gilthead sea bream (Sparus aurata)[J].Aquaculture,2004,220:493-510.

[38] 林海,杜荣.环境温度对肉鸡消化道内食糜排空和消化酶活性的影响[J].动物营养学报,2001,13:49-53.

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