为探讨盐度对瘤背石磺(Onchidium struma)不同消化器官蛋白酶、淀粉酶和纤维素酶活性的影响,试验选用1 200只个体均一的瘤背石磺 随机分成5组,分别置于不同盐度梯度(5、15、25、35和45)下饲养,期间用海泥与螺旋藻粉(m: m=5: 1)拌饲投喂。每组设置3个重复,每个重复80只,饲养30 d后测定消化道不同器官蛋白酶、淀粉酶和纤维素酶活性。试验结果显示:1)各消化酶在幽门胃、贲门胃、肝胰腺和肠中均有分布,但各消化器官之间酶活性差异显著(P<0.05)。不同消化器官中蛋白酶活性由大到小依次为幽门胃、肝胰腺、肠 和贲门胃;淀粉酶活性由大到小依次为肝胰腺、幽门胃、肠 和贲门胃;纤维素酶活性以肝胰腺 最高,其次是肠,最低的是幽门胃。2)盐度对各种消化酶活性的影响差异显著(P<0.05)。除肝胰腺中的蛋白酶以外,幽门胃、贲门胃、肝胰腺和肠中各消化酶活性随盐度升高均呈先上升后下降的趋势,且淀粉酶和纤维素酶分别在盐度15和25时活性最高,盐度45时活性最低。试验结果表明,低盐度(盐度5~25)对各消化器官消化酶活性均具有激活作用;其中,盐度15~25时消化酶活性最高,有利于瘤背石磺对饵料的消化吸收;而当盐度高于25时,则会抑制消化酶活性。
The objective of this study was to evaluate the effects of salinity on the activities of protease, amylase and cellulose in the different digestive organs of Onchidium struma. One thousand and two hundred individuals with an initial average body weight of (16.82?2.14) g were randomly divided into 5 groups with 3 replicates per group and 80 individuals per replicate. The Onchidium struma were raised 30 days under different salinities (5, 15, 25, 35 and 45), fed with sea mud mixed with spirulina powder (m: m=5: 1), and the activities of protease, amylase and cellulose in different digestive organs were measured. The results showed as follows: 1) digestive enzymes were distributed in the pyloric stomach, cardiac stomach, hepatopancreas and intestine, while the digestive enzyme activities among these organs were significantly different (P<0.05). The descending order of protease activity of different digestive organs was pyloric stomach, hepatopancreas, intestine and cardiac stomach, while the amylase activity of hepatopancreas was the highest, followed by pyloric stomach, intestine and cardiac stomach, and the hepatopancreas also owned the highest cellulose activity, followed by the intestine, and the pyloric stomach was the lowest. 2) Salinity had a significant influence on the digestive enzyme activity (P<0.05). The activities of the digestive enzymes of all digestive organs had a trend of decreasing after an initial increase with the salinity rising except the protease activity of hepatopancreas. The activities of amylase and cellulose were the highest at salinity 15 and 25, respectively, and were the lowest at salinity 45. The results indicate that low salinity (salinity 5 to 25) can increase the three digestive enzyme activities in the digestive organs of Onchidium struma; digestive enzyme activity is the highest at salinity 15 to 25, which will be beneficial to the digestion and absorption. However, the digestive enzyme activities may be inhibited when salinity is above 25.
[1] 王云峰,朱鑫华.盐度对鱼类生态生理学特征的影响[J].海洋科学集刊,2002,44:151-158.
[2] 李希国,李加儿,区又君.盐度对黄鳍鲷幼鱼消化酶活性的影响及消化酶活性的昼夜变化[J].海洋水产研究,2006,27(1):40-45.
[3] 张玉玉,王春琳,李来国.长蛸的盐度耐受性及盐度胁迫对其血细胞和体内酶活力的影响[J].台湾海峡,2010,29(4):452-459.
[4] 王兴强,马甡,曹梅,等.不同盐度波动幅度对缢蛏生长和生化组成的影响[J].海洋通报,2010,29(5):567-571.
[5] SINGARAM G,HARIKRISHNAN T,HUANG W B,et al.Immunomodulation in the marine gastropod Haliotis diversicolor exposed to benzo(a)pyrene[J].Chemosphere,2009,75(3):389-397.
[6] WAYNE A O,NORM F L.Salinity and temperature tolerance of embryos and juveniles of the pearl oyster, Pinctada imbricata Röding[J].Aquaculture,2004,229(1/4):493-506.
[7] MCFARLANE I D.Ecology and behaviour of the intertidal pulmonate mollusks Onchidium peronii in Kuwait[J].Journal of University Kuwait,1979,6:169-180.
[8] 王金庆,成永旭,吴旭干,等.瘤背石磺的形态、习性和生殖行为[J].动物学杂志,2005,40(1):32-40.
[9] DESHPANDE U D,NAGABHUSHANAM R,HANUMANTE M M.Reproductive ecology of the marine pulmonate,Onchidium verruculatum[J].Hydrobiologia,1980,71(1/2):83-85.
[10] 黄金田,沈伯平,王资生.瘤背石磺的生态习性观察[J].海洋渔业,2004,26(2):103-109.
[11] 沈和定,陈汉春,陈贤龙,等.石磺繁殖生物学的实验研究[J].水产学报,2006,30(6):753-769.
[12] FRETTER V.Studies in the functional morphology and embryology of Onchidella celtica (Forbes and Hanley) and their bearing on its relationships[J].Journal of the Marine Biological Association of the United Kingdom,1943,25(4):685-720.
[13] YOSHIHIRO M,TSUKASA G,TAKAKO N,et al.Mechanisms of hyperpolarization induced by two cytokines, hTNFα and hIL-1α in neurons of the mollusk,Onchidium[J].Brain Research,1944,653(1):112-118.
[14] HATSUO H,SATORU I.Chaotic nature of bursting discharges in the Onchidium pacemaker neuron[J].Journal of Theoretical Biology,1992,156(3):269-291.
[15] 倪小英,应雪萍,张永普,等.不同温度和pH值对瘤背石磺(Onchidium verruculatum Cuvier)消化酶活性的影响[J].温州大学学报:自然科学版,2009,30(2):1-5.
[16] 黄金田,张余霞.瘤背石磺室内温箱养殖试验[J].海洋科学,2004,26(10):14-16.
[17] 朱俭,曹凯鸣,周润琦,等.生物化学实验[M].上海:上海科学技术出版社,1981:192-193.
[18] 北京大学生物系生物化学教研室.生物化学实验指导[M].北京:高等教育出版社,1979:145-154.
[19] 徐钢春,华丹,顾若波,等.三角帆蚌消化酶的分布特性和晶杆的形态结构[J].水产学报,2008,32(2):296-302.
[20] 倪小英,应雪萍.石磺消化系统的组织学观察[J].四川动物,2009,28(4):553-557.
[21] BINDU R P,DIWAN A D.Effects of acute salinity stress on oxygen consumption and ammonia excretion rates of the marine shrimp Metapenaeus monoceros[J].Journal of Crustacean Biology,2002,22(1):45-52.
[22] DALLA V.Salinity response of the juvenile penaeid shrimp Penaeus japonicus Ⅰ.Oxygen consumption and estimation of productivity[J].Aquaculture,1986,55:297-306.
[23] 江振莹,贾志海,郭锁云,等.稀土元素对鲤鱼肠道发育及消化酶活性的影响[J].水产学报,2007,19(1):86-90.
[24] 杨蕙萍,童圣英,王子臣.皱纹盘鲍蛋白酶的研究[J].水产学报,1997,21(2):128-133.
[25] VEGA V F,NOLASCO H,CIVERA R.The digestive enzymes of the Pacific brown shrimp Penaeus californiensis Ⅰ.Properties of amylase activity in the digestive tract[J].Comparative Biochemistry Physiology,1993,106(3):547-550.
[26] MARANGOS C,BROGREN C H,ALLIOT E.The influence of water salinity on the free amino acid concentration in muscle and hepatopancreas of adult shrimps,Penaeus japonicus[J].Biochemical Systematics and Ecology,1989,17:589-594.
[27] CHEW S F,HO S Y,IP Y K.Free amino acids and osmoregulation in the intertidal pulmonate Onchidium tumidium[J].Marine Biology,1999,134:735-741.
[28] KAPPER M A,STICKLE W B,BLAKENEY E.Volume regulation and nitrogen metabolism in the muricid gastropod Thais haemostoma[J].Biology Bull Marine,1985,169:458-475.
[29] 魏峦峦,沈和定,张雨,等.瘤背石磺线粒体基因组全序列分析[J].水产学报,2011,35(4):493-500.
[30] DEATON L E.Hyperosmotic cellular volume regulation in the ribbed mussel Geukensia demissa:inhibition by lysosomal and proteinase inhibitors[J].Journal of Experimental Zoology,1987,244:375-382.
[31] 辛碧芬,谢进金,左巧阳.几种金属离子对鲢肝胰脏淀粉酶的影响[J].水生态学杂志,2009,2(3):91-95.
[32] 吴众望,潘鲁青,董双林.9种金属离子对缢蛏消化酶活力的影响[J].中国水产科学,2003,10(4):297-300.
[33] 尾崎久雄.鱼类消化生理(上、下册)[M].李爱杰,沈宗武,译.上海:上海科学技术出版社,1985.
[34] CHIU Y N,BENITEZ L V.Studies on the carbohydrates in the digestive tract of the Milkfish chanos chanos[J].Marine Biology,1981,61:247-254.
[35] 李俊辉,王庆恒,杜晓东,等.温度和pH对马氏珠母贝肝胰脏消化酶活力的影响[J].水产科学,2011,30(2):115-117.
[36] 吴永沛,蔡慧农.金属离子和pH值对九孔鲍几种消化酶活力的影响[J].海洋科学,2003,27:66-69.
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