本试验旨在研究无氮饲粮对14日龄和35日龄肉鸡盲肠微生物菌群结构及内源氨基酸基础损失量的影响。分别选用10日龄和31日龄罗斯(Ross)308雄性肉鸡,随机分为2个组:常规饲粮组和无氮饲粮组,每组5个重复,其中10日龄时每个重复12只鸡,31日龄时每个重复6只鸡,试验期均为4 d。结果表明:肉鸡14日龄和35日龄时,收粪法所得大多数氨基酸内源基础损失量均显著或极显著高于回肠食糜法(P<0.05或P<0.01)。相对常规饲粮,无氮饲粮改变了14日龄和35日龄肉鸡盲肠微生物16SrDNA V3高变区变性梯度凝胶电泳图谱的优势条带,其中14日龄和35日龄发生变化的优势条带灰度值占其整个优势条带总灰度值的比例分别为72%、39%。与常规饲粮相比,无氮饲粮对14日龄肉鸡盲肠食糜重、氨态氮浓度没有显著影响(P>0.05),但极显著降低了盲肠食糜中丁酸浓度(P<0.01),显著降低了异丁酸和戊酸浓度(P<0.05),极显著增加了丙酸浓度(P<0.01)。无氮饲粮组35日龄肉鸡盲肠食糜重极显著高于常规饲粮组(P<0.01),盲肠食糜氨态氮、乙酸、异丁酸、戊酸、异戊酸浓度则极显著降低(P<0.01)。综上所述,采用无氮饲粮测定内源氨基酸基础损失量时,收粪法所得大多数氨基酸内源基础损失量显著高于回肠食糜法。饲喂无氮饲粮4 d后,肉鸡盲肠食糜原有微生物优势菌群发生改变。
The purpose of this study was to explore the effects of nitrogen-free diets on cecal microflora and endogenous amino acid basal losses of broilers aged 14 and 35 days. A total of 308 Male Ross broiler chickens, aged 10 and 31 days, respectively, were chosen and randomly assigned into two dietary treatments, in which one was fed a conventional diet and the other was fed a nitrogen-free diet for 4 days. Every treatment contained five replicates with 12 chickens on day 10 and 6 chickens on day 31 per replicate. The results showed that endogenous basal losses of most amino acids determined by excreta-based assay were significantly higher than those determined by ileum digesta-based assay in broilers aged 14 and 35 days (P<0.05 or P<0.01). Compared with the conventional diet, there were some changes of DGGE predominant bands of cecal microflora 16S rDNA V3 region in broilers fed the nitrogen-free diet aged 14 and 35 days, the 72% and 39% of total gray value of predominant bands changed respectively for broilers fed the nitrogen-free diet aged 14 and 35 days. On day 14, there was no significant difference in cecal digesta weight and ammoniacal nitrogen concentration in broilers between nitrogen-free diet group and conventional diet group (P>0.05), but the concentrations of butyric acid (P<0.01), isobutyric acid (P<0.05) and valeric acid (P<0.05) in cecum digesta of broilers were significantly decreased and propionic acid concentration was significantly increased (P<0.01) by the nitrogen-free diet compared with the conventional diet. On day 35, compared with conventional diet group, cecal digesta weight of broilers in the nitrogen-free diet group was significantly increased, and the concentrations of ammoniacal nitrogen, acetic acid, isobutyric acid, valeric acid and isovaleric acid were significantly decreased (P<0.01). In conclusion, endogenous basal losses of most amino acids measured by excreta-based assay are higher than those measured by ileal digesta-based assay when broilers are fed the nitrogen-free diet. Some predominant species of cecal microflora should change after broilers are fed the nitrogen-free diet for 4 days.
[1] ADEDOKUN S A,ADEOLA O,PARSONS C M,et al.Factors affecting endogenous amino acid flow in chickens and the need for consistency in methodology[J].Poultry Science,2011,90(8):1737-1748.
[2] PARSONS C M,POTTER L M,BROWN R D.Effects of dietary carbohydrate and of intestinal microflora on excretion of engogenous amino acid by poultry[J].Poultry Science,1983,62(3):483-489.
[3] SALTERA D N,FULFORDA R J.The influence of the gut microflora on the digestion of dietary and endogenous proteins:studies of the amino acid composition of the excreta of germ-free and conventional chicks[J].British Journal of Nutrition,1974,32(3):625-637.
[4] KADIM I T,MOUGHAN P J,RAVINDRAN V.Ileal amino acid digestibility assay for the growing meat chicken-comparison of ileal and excreta amino acid digestibility in the chicken[J].British Poultry Science,2002,43(4):588-597.
[5] ZHOU H,GONG J,BRISBIN J T,et al.Appropriate chicken sample size for identifying the composition of broiler intestinal microbiota affected by dietary antibiotics,using the polymerase chain reaction-denaturing gradient gel electrophoresis technique[J].Poultry Science,2007,86(12):2541-2549.
[6] SHORT F J,WISEMANA G P,BOORMAN K N.Determination of titanium dioxide added as an marker in chicken digestibility studies[J].Animal Feed Science and Technology,1996,59(4):215-221.
[7] 邓雪娟,刘国华,蔡辉益,等.分光光度计法测定禽饲料和食糜中二氧化钛[J].饲料工业,2008,29(2):57-58.
[8] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and amino acids in ruminal fluids and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.
[9] TAYLOR R D,JONES G P D.The incorporation of whole grain into pelleted broiler chicken diets.Ⅱ.Gastrointestinal and digesta characteristics[J].British Poultry Science,2004,45(2):237-246.
[10] 高巍.猪胃肠道正常微生物区系及其凝集素活性与性质.硕士学位论文.北京:中国农业大学,2001.
[11] TANIKAWA T S,SONOHARA N,SAITO N,et al.Aging transition of the bacterial community structure in the chick ceca[J].Poultry Science,2011,90(5):1004-1008.
[12] YU Z T,MARK M.Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis[J].Applied and Environmental Microbiology,2004,70(8):4800-4806.
[13] ADEDOKUN S A,PARSONS C M,LILBURN M S.Endogenous amino acid flow in broiler chicks is affected by the age of birds and method of estimation[J].Poultry Science,2007,86(12):2590-2597.
[14] SELLE P,RAVINDRAN V,BRYDEN W L,et al.Influence of dietary phytate and exogenous phytase on amino acid digestibility in poultry:a review[J].The Journal of Poultry Science,2006,43:89-103.
[15] OBERLEAS D,LI Y C,STOECKER J,et al.The rate of chromium transit through the gastrointestinal tract[J].Nutrition Research,1990(10):1189-1194.
[16] MOUGHAN P J,DONKOH A.Amino acid digestibility in non-ruminants:a review[C]//FARREL D J,ed.Recent advances in animal nutrition in Austrilia.Armidale,NSW(New South Wales):Australia University of New England,1991:172-184.
[17] GOLIAN A,GUENTER W,HOEHLER D,et al.Comparison of various methods for endogenous ileal amino acid flow determination in broiler chickens[J].Poultry Science,2008,87(4):706-712.
[18] RAVINDRAN V,HENDRIKS W H.Recovery and composition of endogenous protein collected at the terminal ileum as influenced by the age of broiler chickens[J].Australian Journal of Agricultural Research,2004,55(6):705-709.
[19] LACEY R E,REDWINE J S,PARNELL C B.Particulate matter and ammonia emisson factors for tunnel-ventilated broiler production houses in the southern US[J].American Society of Agricultural Engineers,2003,46(4):1203-1214.
[20] KARASAWA Y.Ammonia production from uric acid,urea and amino acids and its absorption from the ceca of the cockerel[J].Journal of Experimental Zoology,1989,252(S3):75-80.
[21] MOAT A G,FOSTER J W,SPECTOR M P,et al.微生物生理学[M].4版.李颖,文莹,关国华,等译.北京:高等教育出版社,2009:347.
[22] MISIR R,SAUER W C.Effect of starch infusion at the terminal ileum on nitrogen balance and apparent digestibilities of nitrogen and amino acids in pigs fed meat-and-bone and soybean meal diets[J].Journal of Animal Science,1982,55(3):599-607.
[23] PARSONS C M,POTTER L M,BROWN R D.True metabolizable energy and amino acid digestibility of dehulled soybean meal[J].Poultry Science,1981,60(12):2687-2696.
[24] CRESSMAN M D.A molecular approach to understanding the interrelation between the microbiomes in the litter and intestines of commercial broiler chickens.Master degree thesis.Columbus:Ohio State University,2009:18-25.
[25] KLAPPENBACH J A,DUNBAR J M,SCHMIDT T M.rRNA operon copy number reflects ecological strategies of bacteria[J].Applied And Environmental Microbiology,2000,66(4):1328-1333.
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