本文旨在探讨饲粮蛋氨酸添加水平对断奶至3月龄獭兔生长性能、免疫功能及血清生化指标的影响。选用30日龄断奶生长獭兔200只,随机分成5组,每组40个重复,每个重复1只兔。各组分别饲喂在基础饲粮中添加不同水平(0、0.15%、0.30%、0.45%、0.60%)蛋氨酸的试验饲粮。预试期7 d,正试期53 d。结果表明:1)饲粮蛋氨酸添加水平对平均日采食量影响不显著(P>0.05);对平均日增重影响显著(P<0.05),在0.45%添加组时达到最大值16.76 g/d;对料重比影响极显著(P<0.01),在0.45%添加组时达到最小值3.55。2)饲粮蛋氨酸添加水平对摄入氮、粪氮、尿氮、可消化氮、沉积氮、氮表观消化率、氮利用率和氮生物利用率均无显著影响(P>0.05)。3)饲粮蛋氨酸添加水平对血清免疫球蛋白A、免疫球蛋白G和免疫球蛋白M含量均无显著影响(P>0.05)。4)饲粮蛋氨酸添加水平对血清总蛋白、白蛋白、甘油三酯含量及碱性磷酸酶活性均无显著影响(P>0.05),但极显著影响血清尿素氮含量(P<0.01),其中0.30%和0.45%添加组极显著低于0和0.15%添加组(P<0.01),在0.45%添加组时达到最小值5.70 mmol/L。综合考虑本试验所测指标,断奶至3月龄獭兔的蛋氨酸适宜添加水平为0.45%(饲粮总蛋氨酸水平为0.68%)。
This experiment was conducted to study the effects of dietary methionine (Met) supplemental level on growth performance, immune function and serum biochemical indices of weaner to 3-month-old growing Rex rabbits. Two hundred weaner Rex rabbits were randomly assigned to 5 groups with 40 replicates in each group and each replicate contained 1 rabbit. Rabbits in 5 groups were fed a basal diet supplemented with 0,0.15%,0.30%,0.45% and 0.60% Met,respectively. The trial lasted for 7 days for adaptation, and 53 days for experimental period. The results showed as follows:1) dietary Met supplemental level did not significantly affected average daily intake (P>0.05), but significantly affected average daily gain (P<0.05), the average daily gain in 0.45% Met group was the highest (16.76 g/d), and extremely significantly affected feed to gain (P<0.01), the feed to gain in 0.45% Met group has the minimum value (3.55). 2) Dietary Met supplemental level had no significant influence on nitrogen intake, fecal nitrogen, urinary nitrogen, digestible nitrogen, nitrogen retention, nitrogen apparent digestibility, nitrogen utilization rate and nitrogen biological availability (P>0.05). 3) Dietary Met supplemental level had no significant influence on the contents of IgA, IgM and IgG in serum (P>0.05). 4) Dietary Met supplemental level had no significant influence on the contents of total protein, albumin and triglyceride and alkaline phosphatase activity in serum (P>0.05), but had extremely significant influence on serum urea nitrogen content (P<0.01). The serum urea nitrogen content in the groups supplemented with 0.30%, 0.45% Met was extremely lower than that in the groups supplemented with 0 and 0.15% Met, and the minimum value (5.70 mmol/L) was in 0.45% Met group. In conclusion, considering all indices of this experiment, the appropriate Met supplemental level in diet of weaner to 3-month-old growing Rex rabbit is 0.45% (the actual Met content in diet is 0.68%).
[1] CAREW L B,MC MURTRY J P,ALSTER F A.Effects of methionie deficiencies on plasma levels of thyroid hormones,insuline-like growth factors-Ⅰ and -Ⅱ,liver and body weights,and feed intake in growing chickens[J].Poultry Science,2003,82:1932-1938.
[2] GAINES A,RATLIFF B,SRICHANA P,et al.Methionine sources studied[J].National Hog Farmer,2004,49:26.
[3] 乔德堂.动物的蛋氨酸营养研究进展[J].山东畜牧兽医,2007,28(4):57-58.
[4] WICKER D L.Methionine'past[J].Feed Manage,1990,41:64-66.
[5] NRC.Nutrient requirements of rabbits[S].Washington,D.C.:National Academy Press,1977.
[6] DE BLAS C,MATEOS G G.Feed formulation[M]//DE BLAS,WISEMAN J J.The nutrition of the rabbit.New York:CABI Publishing,1998:241-253.
[7] 张丽英.饲料分析及质量检测技术[M].北京:中国农业大学出版社,2002:49-53.
[8] EL BOUSHY A R,POEL A F B,ROODBEEN A E.Broiler response to low-protein diets supplemented with oleandomycin in relation to synthetic methionine and lysine[J].Feedstuffs,1919,51:20-21.
[9] 张永翠,李福昌.日粮添加蛋氨酸对幼兔生长发育、免疫性能及血液生化指标的影响[J].中国养兔,2008,3(3):13-16.
[10] 周勤飞,肖文才,王永才.饲粮蛋氨酸水平对新西兰肉兔生长性能和毛皮品质的影响[J].动物营养学报,2011,23(1):73-77.
[11] 李天俊,宋桂敏,周淑云,等.肉兔添加蛋氨酸肥育实验[J].中国养兔杂志,2005(1):7-8.
[12] SPREADBURY D.A study of the protein and amino acid requirements of the growing New Zealand while rabbit with emphasis on lysine and the sulphur-containing amino acids[J].British Journal of Nutrition,1978,39:601-613.
[13] AHMED B M,ABOU-ASHOUR A M.Protein quality and amino acid requirement of Baladi rabbits[J].World Review of Animal Production,1984,55:58-60.
[14] SUSANNA C R,DEBORAH H L.Nutrition and the immune system of the gut[J].Nutrition,1998,14:573-579.
[15] CALDER P C,PHLIP C.Nutrition and immunity function[J].Nutrition Clinique et Metabolism,2001,15(4):286-297.
[16] TSIAGBE V K.Enhanced immune responses of broiler chicks fed methionine supplemented diets[J].Poultry Science,1987,58:1147-1154.
[17] 李振,李萍.蛋白质营养与家禽免疫[J].中国饲料,2002(18):22-23.
[18] 伍喜林,杨忠源.日粮不同苏氨酸水平对仔猪生产性能和血液生化指标的影响[J].中国畜牧杂志,1994,5(30):10-12.
[19] 赵国先,张正珊.低蛋白饲粮加蛋氨基酸对肉兔生产性能及血液生化指标的影响[J].饲料与畜牧,1997(2):9-11.
[20] MALMOLF K.Amino acid in farm animal nutrition metabolism,partition and consequences of imbalance[J].Swedish Journal of Agriculture Research,1988,18(4):191-193.
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