本试验旨在研究在小麦基础饲粮中添加木聚糖酶对生长猪生长性能、营养物质表观消化率、血清生化指标及肠道微生物菌群的影响,探讨适宜的木聚糖酶添加水平。选择80头健康、平均体重40kg的"杜×长×大"生长猪,随机分为5个处理,每个处理4个重复,每个重复4头猪。正对照组饲喂玉米-豆粕型基础饲粮,负对照组饲喂小麦-豆粕型基础饲粮,木聚糖酶组分别饲喂在负对照基础饲粮上添加500、1000和2000U/kg木聚糖酶的试验饲粮。试验期28d。结果表明:1)小麦基础饲粮中添加木聚糖酶可极显著提高生长猪平均日增重(P<0.01),显著或极显著提高平均日采食量和营养物质表观消化率(P<0.05或P<0.01),其中2000U/kg木聚糖酶组效果最好,平均日增重和平均日采食量分别提高了26.0%和16.5%(P<0.01);2)小麦基础饲粮中添加木聚糖酶显著或极显著提高了生长猪血清葡萄糖、三碘甲腺原氨酸、四碘甲腺原氨酸和D-木糖含量(P<0.05或P<0.01),极显著降低血清尿素氮含量(P<0.01);3)饲粮组成和木聚糖酶可显著影响猪肠道微生物菌群,添加木聚糖酶可维持动物肠道微生物菌群的平衡,2000U/kg木聚糖酶组肠道菌群结构和玉米-豆粕饲粮饲喂条件下接近。由此可见,添加2000U/kg的木聚糖酶可显著改善饲喂小麦基础饲粮生长猪的生长性能和营养物质表观消化率,维持肠道微生物菌群的平衡。在木聚糖含量为11.2%的生长猪小麦基础饲粮中,木聚糖酶的适宜添加水平为2000U/kg。
This experiment was conducted to investigate the effects of wheat-based diets supplemented with xylanase on growth performance, nutrient apparent digestibility ,serum biochemical parameters and intestinal microbial flora of growing pigs, and to explore the optimal level of xylanase. A total of eighty healthy crossbred pigs (Duroc譒andrace譒arge) with an average bodyweight of 40 kg were randomly allotted into 5 treatments with 4 replicates per treatment and 4 pigs per replicate. Pigs in positive control group were fed the corn-soybean basal diet, pigs in negative control group were fed the wheat-soybean basal diet, and the others in xylanase groups were fed the wheat-soybean basal diet supplemented with 500, 1 000 and 2 000 U/kg xylanase, respectively. The experiment lasted for 28 days. The results showed as follows:1) the wheat-soybean basal diet supplemented with xylanase significantly increased the average daily gain (P<0.01), average daily feed intake (P<0.05) and nutrient apparent digestibility (P<0.05) of growing pigs, and the best results were obtained at 2 000 U/kg xylanase group, the average daily gain and average daily feed intake were increased by 26.0% and 16.5% (P<0.01). 2) The wheat-soybean basal diet supplemented with xylanase significantly increased the contents of glucose, triiodothyronine, thyroxine, and D-xylose in serum of growing pigs (P<0.05, P<0.01), while significantly decreased the serum urea nitrogen content (P<0.01). 3) The intestinal microbial flora was affected by dietary composition and xylanase, diets supplemented with xylanase could maintain the microflora balance for pigs, and the composition of intestinal flora by adding 2 000 U/kg xylanase was similar to the corn-soybean basal diet. In conclusion, adding 2 000 U/kg xylanase can significantly improve the growth performance and nutrient apparent digestibility of pigs that fed with wheat-soybean basal diet, and maintain the balance of intestinal flora. The optimal level of xylanase for growing pigs in the wheat-soybean basal diet (containing 11.2% xylan) is 2 000 U/kg.
[1] 谭权,张克英.木聚糖的抗营养作用[J].中国家禽,2008,30(12):55-57.
[2] 丁强.饲用木聚糖酶的研究进展及应用[J].中国饲料,2010(3):22-25.
[3] OWUSU-ASIEDU A,SIMMINS P H,BRUFAU J,et al.Effect of xylanase and -glucanase on growth performance and nutrient digestibility in piglets fed wheat-barley-based diets[J].Livestock Science,2010,134:76-78.
[4] BARRERA M,CERVANTES M,SAUER W C,et al.Ileal amino acid digestibility and performance of growing pigs fed wheat-based diets supplemented with xylanase[J].Journal of Animal Science,2004,82(7):1997-2003.
[5] 罗定媛,杨凤霞,张利敏,等.小麦型日粮添加木聚糖酶对肉鸡生产性能和血液生化指标的影响[J].西北农林科技大学学报:自然科学版,2009(5):53-58.
[6] MOEHN S,ATAKORA J K A,SANDS J,et al.Effect of phytase-xylanase supplementation to wheat-based diets on energy metabolism in growing-finishing pigs fed ad libitum[J].Livestock Science,2007,109(1):271-274.
[7] WOYENGO T A,SANDS J S,GUENTER W,et al.Nutrient digestibility and performance responses of growing pigs fed phytase-and xylanase-supplemented wheat-based diets[J].Journal of Animal Science,2008,86(4):848-857.
[8] 黄金秀,陈代文,张克英.木聚糖酶对不同木聚糖含量的仔猪饲粮养分消化率的影响[J].中国畜牧杂志,2008,44(7):21-24.
[9] 王金全,蔡辉益,陈宝江.小麦日粮中添加木聚糖酶对肉仔鸡生产性能、免疫、消化器官发育和血液代谢激素水平的影响[J].河北农业大学学报,2005,28(1):73-76.
[10] LARSEN F M,MOUGHAN P J,WILSON M N.Dietary fiber viscosity and endogenous protein excretion at the terminal ileum of growing rats[J].The Journal of Nutrition,1993,123(11):1898.
[11] KITTELMANN S,JANSSEN P H.Characterization of rumen ciliate community composition in domestic sheep,deer,and cattle,feeding on varying diets,by means of PCR-DGGE and clone libraries[J].FEMS Microbiology Ecology,2011,75(3):468-481.
[12] SIMON O.The mode of action of NSP hydrolysing enzymes in the gastrointestinal tract[J].Journal of Animal and Feed Sciences,1998(7):115-123.
[13] VAHJEN W,GLSER K,SCHFER K,et al.Influence of xylanase-supplemented feed on the development of selected bacterial groups in the intestinal tract of broiler chicks[J].The Journal of Agricultural Science,1998,130(4):489-500.
[14] 叶楠,陈代文,余冰,等.不同木聚糖水平饲粮中添加木聚糖酶对断奶仔猪生长性能及肠道微生态环境的影响[J].动物营养学报,2011,23(11):1961-1969.
[15] 王俊丽,聂国兴.低聚木糖与肠道微生态研究进展[J].世界华人消化杂志,2011,19(7):710-717.
[16] 王继成,潘灵辉,李淑云.低聚木糖对断奶仔猪生产性能,肠道菌群及免疫水平影响的研究[J].中国畜牧兽医,2006,33(5):3-7.
[17] 赵静杰,李小成,王景儒.低聚糖对断奶仔猪生产性能的影响[J].粮食与饲料工业,2008(1):34-35.
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