本试验旨在研究饲粮ω-6/ω-3多不饱和脂肪酸(PUFA)比例对鹅肝脏过氧化脂质和肝细胞超微结构的影响。选择160只同批出雏、体质健壮、体重(0.407±0.023) kg的21日龄扬州鹅,随机分成4组,每组4个重复,每个重复10只,分别饲喂ω-6/ω-3 PUFA比例为12:1、9:1、6:1、3:1的试验饲粮。饲养至42、56和70日龄时分别进行肝脏分离和过氧化脂质测定,并采用电镜观察肝细胞超微结构。结果表明:鹅肝脏丙二醛(MDA)、一氧化氮(NO)含量随日龄的增加呈上升趋势,并以70日龄较高,其中12:1和9:1组升高的幅度较大,而3:1和6:1组较小。MDA、NO含量随ω-6/ω-3 PUFA比例的降低呈现下降的趋势,各日龄基本以3:1组最低。12:1和9:1组肝细胞糖原颗粒相对减少,线粒体有肿胀现象,但各组线粒体的面积没有显著变化(P>0.05)。由此可见,生长期扬州鹅肝脏抗氧化状态受饲粮ω-6/ω-3 PUFA比例的影响;较低饲粮ω-6/ω-3 PUFA比例(3:1和6:1)能够减少肝细胞过氧化脂质的损伤。
The objective of this study was to investigate the effects of dietary ω-6/ω-3 polyunsaturated fatty acid (PUFA) ratios on lipid peroxide and cellular ultrastructure in geese liver. A total of 160 healthy Yangzhou geese with similar weight (0.407±0.023) kg, aged 21 days, were randomly divided into 4 groups with 4 replicates per group and 10 geese per replicate. They were fed the diets with ω-6/ω-3 PUFA ratios of 12:1, 9:1, 6:1, and 3:1, respectively. The liver was separated and lipid peroxides were tested at 42, 56, and 70 days of age. The results showed as follows:the contents of malondialdehyde (MDA) and nitric oxide (NO) in liver showed an ascendant tendency with age increasing, and the highest was at 70 days of age. The increasing ranges of 12:1 and 9:1 groups were higher than these of 6:1 and 3:1 groups. It was also observed that the contents of MDA and NO showed a declined tendency with the decreasing of ω-6/ω-3 PUFA ratios, and the lowest was in 3:1 group regardless the detected ages. The cellular ultrastructure observation showed that dietary ω-6/ω-3 PUFA ratios in 12:1 and 9:1 groups decreased the number of hepatocyte glycogen pellets, and swelled mitochondria; but there was no significant difference in the average area of mitochondria among all groups (P>0.05). In conclusion, dietary ω-6/ω-3 PUFA ratio affects the hepatic antioxidant function in growing Yangzhou geese, and dietary lower ω-6/ω-3 PUFA ratio, such as 3:1 and 6:1, can decrease hepatocytes damage of lipid peroxide.
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