本试验旨在研究内蒙古呼和浩特地区不同生育期柠条锦鸡儿营养成分的动态变化及其在绵羊瘤胃内的降解特性。分别于孕蕾期、开花期和结实期采集当年生柠条锦鸡儿嫩枝条,测定初水分后在65℃烘箱中烘干,测定常规营养物质含量;选用安装有永久性瘤胃瘘管的内蒙古小尾寒羊3只,采用瘤胃尼龙袋法评定不同生育期柠条锦鸡儿营养成分的瘤胃降解特性。试验结果表明:1)开花期和结实期柠条锦鸡儿的干物质(DM)和粗蛋白质(CP)含量显著高于孕蕾期(P<0.05);不同生育期柠条锦鸡儿有机物(OM)、粗脂肪和无氮浸出物含量无显著差异(P>0.05);结实期粗灰分含量显著高于孕蕾期和开花期(P<0.05);孕蕾期和开花期柠条锦鸡儿中性洗涤纤维(NDF)含量显著高于结实期(P<0.05),而酸性洗涤纤维(ADF)含量显著低于结实期(P<0.05);随着生育期的延续,柠条锦鸡儿木质素含量逐渐升高,开花和结实期显著高于孕蕾期(P<0.05);随着生育期的延续,柠条锦鸡儿钙含量显著提高(P<0.05),磷含量显著降低(P<0.05)。2)随着生育期的延续,柠条锦鸡儿DM的72h消失率和有效降解率逐渐降低,开花期和结实期DM的有效降解率无显著差异(P>0.05);不同生育期柠条锦鸡儿CP的72 h消失率无显著差异(P>0.05),但有效降解率孕蕾期显著高于开花期和结实期(P<0.05);随着生育期的延续,柠条锦鸡儿OM和NDF的72h消失率和有效降解率均显著降低(P<0.05);开花期和结实期柠条锦鸡儿ADF的72h消失率和有效降解率无显著差异(P>0.05),但显著低于孕蕾期(P<0.05)。试验结果提示,结合不同生育期柠条锦鸡儿营养物质含量、 饲草DM产量以及营养物质的瘤胃降解特性,开花期柠条锦鸡儿营养物质的利用效率最高,为最佳刈割时期。
This experiment was conducted to investigate the dynamic change and rumen degradation characteristics of Caragana korshinskii nutrients in different growth periods in Hohhot, Inner Mongolia. Twigs of Caragana korshinskii were mowed in budding, flowering and fructicative periods, respectively, and the nutrients were analyzed after determination of initial moisture and then drying in the oven at 65 ℃. Three sheep fixed with permanent rumen fistulas were selected and the rumen nylon bag technique was used to study the rumen degradation characteristics of Caragana korshinskii nutrients in different growth periods. The results showed as followed:1) the contents of dry matter (DM) and crude protein (CP) of Caragana korshinskii in flowering and fructicative periods were significantly higher than those in budding period (P<0.05); there were no significant differences in the contents of ether extract, nitrogen free extract and organic matter (OM) among budding, flowering and fructicative periods (P>0.05); the crude ash content in fructicative period was significantly higher than that in budding and flowering periods (P<0.05); the neutral detergent fiber (NDF) content in budding and flowering periods was significantly increased compared with that in fructicative period, however, the acid detergent fiber (ADF) content was significantly decreased (P<0.05); the lignin content was increased with the extend of growth period, and the lignin content in the flowering and fructicative periods was significantly increased compared with that in budding period (P<0.05); the Ca content was significantly increased (P<0.05) but the P content was significantly decreased (P<0.05) with the extend of growth period. 2) Rumen DM disappearance rate at 72 h and effective degradation rate were decreased gradually with the extend of growth period, and effective degradation rate in budding period was significantly higher than that in flowering and fructicative periods (P<0.05); there were no significant effects for growth period on rumen CP disappearance rate at 72 h (P>0.05), however, effective degradation rate in budding period was significantly higher than that in flowering and fructicative periods (P<0.05); with the extend of growth period, rumen disappearance rate at 72 h and effective degradation rate of OM and NDF were decreased significantly (P<0.05); there were no significant differences in rumen disappearance rate at 72 h and effective degradation rate of ADF between flowering and fructicative periods (P>0.05), but they were all significantly lower than those in budding period (P<0.05). It is concluded that Caragana korshinskii has a higher utilization rate in flowering period and it is appropriate for mowing in this period considering nutritive components, grass DM production and rumen degradation characteristics of Caragana korshinskii in different growth periods.
[1] 弓剑,曹社会.柠条饲料的营养价值评定研究[J].饲料博览:技术版,2008(1):53-55.
[2] 王聪,黄应祥,刘强,等.柠条饲喂荷斯坦育成母牛效果的研究[J].饲料与畜牧,2004(6):10-12.
[3] 吴建国,高雁峰,张晓春.不同刈割期柠条饲喂绵羊试验效果的研究[C]//第四届中国畜牧科技论坛论文集.重庆:中国畜牧兽医学会,2009:662-666.
[4] 罗惠娣,牛西午,毛杨毅,等.柠条饲喂山羊效果试验[J].草业科学,2006,23(4):63-66.
[5] 王爱武.柠条饲喂家兔效果试验[J].安徽农学通报,2008,14(23):147.
[6] 魏建秋,王海,贾喜棉,等.河北坝上地区柠条营养价值分析[J].河北林果研究,2009,24(2):131-133.
[7] 王聪,刘强,黄应祥,等.刈割时间与加工方法对柠条营养价值的影响[J].中国畜牧杂志,2006,42(7):54-56.
[8] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:49-74.
[9] 颜品勋,冯仰廉,王燕兵,等.青粗饲料通过牛瘤胃外流速度的研究[J].动物营养学报,1994,6(2):20-22.
[10] 刘强,董宽虎,刘明祥.刈割时间与加工方法对柠条营养价值的影响[J].草地学报,2005,13(2):121-125.
[11] 许冬梅,王玲,崔慰贤,等.治沙灌木主要养分及在羊瘤胃的降解率和体外消化率[J].宁夏农林科技,1999(4):31-33.
[12] 项锴锋,朝鲁孟其其格,贾玉山.柠条微贮前后饲用价值评价[J].内蒙古草业,2005,17(3):27-30.
[13] 马文智,文学飞,李爱华,等.几种处理柠条干物质在滩羊瘤胃内的降解[J].饲料工业,2005,26(2):43-46.
[14] 温学飞,马文智,李红兵,等.几种处理对柠条养分的影响及其在瘤胃内的降解[J].草业科学,2006,23(2):38-42.