本试验旨在研究枯草芽孢杆菌B1对饲喂高脂饲粮小鼠抗氧化功能的影响。将45只体重相近 的普通ICR小鼠随机分为对照组(基础饲粮)、高脂组(高脂饲粮)、试验组 ,每组3个重复,每个重复5只,饲养30 d。观察枯草芽孢杆菌B1对高脂饲粮小鼠肠黏膜、肝脏和血清的抗氧化能力、氧化应激状态的影响。结果表明:1)与对照组相比,高脂组小鼠的肠黏膜总抗氧化能力(T-AOC)显著减少了28.34%(P<0.01),肝脏超氧化物歧化酶、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和硫氧还蛋白还原酶活性分别显著降低了22.91%(P<0.05)、37.35%(P<0.01)、47.47%(P<0.01)和36.42%(P<0.05);血清还原型谷胱甘肽(GSH)含量、GSH-Px和抗超氧阴离子自由基(O₂^-·)活性分别显著下降了30.73%(P<0.01)、47.26%(P<0.01)和8.02%(P<0.01);肝脏8-羟基脱氧鸟苷(8-OhdG)含量显著增加了67.87%(P<0.01)。2)与高脂组相比,试验组小鼠的肠黏膜T-AOC显著增加了32.44%(P<0.05),肝脏GSH含量和CAT活性分别显著上升了38.27%(P<0.05)和53.01%(P<0.05);血清T-AOC、GSH含量、GSH-Px和抗O₂^-·活性分别显著提高了10.72%(P<0.05)、38.01%(P<0.05)、51.98%(P<0.01)和13.45%(P<0.01);肝脏丙二醛、8-OHdG含量和黄嘌呤氧化酶活性分别显著下降了16.01%(P<0.05)、37.52%(P<0.01)和16.25%(P<0.05)。由此可见,高脂饲粮降低了小鼠肠黏膜、肝脏和血清的抗氧化功能;添加枯草芽孢杆菌B1对饲喂高脂饲粮小鼠的抗氧化功能具有一定的增强作用,并能减少机体的氧化应激状态,较好地保护组织细胞,免受氧化损伤。

The aim of this study was to investigate the effects of Bacillus subtilis B1 on antioxidant function of mice fed a high-fat diet. Forty-five ICR mice with similar weight were randomly divided into three groups, control group (fed a basal diet), high-fat group (fed a high-fat diet) and experimental group , with 3 replicates per group and 5 mice per replicate. The mice were raised for 30 days to observe the effects of Bacillus subtilis B1 on the antioxidant capacity and oxidative stress in their intestinal mucosa, liver and serum. The results showed as follows:1) compared with the control group, intestinal mucosal total antioxidant capacity (T-AOC), liver superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and thioredoxin reductase (TrxR) activities and serum glutathione (GSH) content, GSH-Px and anti-superoxide anion radical (anti-O₂^-?) activities in the high-fat group were significantly decreased by 28.34% (P<0.01), 22.91% (P<0.05), 37.35% (P<0.01), 47.47% (P<0.01), 36.42% (P<0.05), 30.73% (P<0.01), 47.26% (P<0.01) and 8.02% (P<0.01), respectively, whereas liver 8-hydroxydeoxyguanosine (8-OHdG) content in the high-fat group was remarkably increased by 67.87% (P<0.01). 2) Compared with the high-fat group, intestinal mucosal T-AOC, liver GSH content and CAT activity and serum T-AOC, GSH content, GSH-Px and anti-O₂^-? activities in the experimental group were significantly increased by 32.44% (P<0.05), 38.27% (P<0.05), 53.01% (P<0.05), 10.72% (P<0.05), 38.01% (P<0.05), 51.98% (P<0.01) and 13.45% (P<0.01), respectively, whereas liver malondialdehyde (MDA) and 8-OHdG contents, and xanthine oxidase (XOD) activity in the experimental group were remarkably reduced by 16.01% (P<0.05), 37.52% (P<0.01) and 16.25% (P<0.05), respectively. It is concluded that the high-fat diet can reduce antioxidant capacity and also cause oxidative stress in the intestinal mucosa, liver and serum of mice, and the supplementation of Bacillus subtilis B1 can enhance the antioxidant function of the mice fed the high-fat diet, reduce oxidative stress in their bodies, and protect their cells from oxidative damage.