动物营养学报 >

2012 , Vol. 24 >Issue 12: 2375 - 2383

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.12.012

水产营养 Fish Nutrition

投喂高脂饲料后草鱼主要生化指标和乙酰辅酶A 羧化酶1 mRNA表达的变化

  • 汪开毓 ,
  • 苗常鸿 ,
  • 黄锦炉 ,
  • 王均 ,
  • 连海 ,
  • 吴春艳 ,
  • 牟巧凤 ,
  • 付希
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  • 四川农业大学鱼病研究中心,四川农业大学动物疫病与人类健康四川省重点实验室,雅安 625014

收稿日期: 2012-07-24

  网络出版日期: 2012-12-03

基金资助

教育部长江学者和创新团队发展计划创新团队项目(IRT0848)

收起

Changes in Main Biochemical Indices and Acetyl-CoA Carboxylase 1 mRNA Expression of Crass Carp Fed a High-Fat Diet

  • WANG Kaiyu ,
  • MIAO Changhong ,
  • HUANG Jinlu ,
  • WANG Jun ,
  • LIAN Hai ,
  • WU Chunyan ,
  • MOU Qiaofeng ,
  • FU Xi
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  • Fisheries Disease Research Center, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China

Received date: 2012-07-24

  Online published: 2012-12-03

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摘要

为了研究草鱼肝脏对高脂饲料的代谢调控机理,试验研究了连续12周投喂含8.1%脂肪的饲料对草鱼血清生化指标、肝胰脏生化指标及乙酰辅酶A羧化酶1(ACC1)mRNA表达的影响。将120尾平均体重为(15.0±2.4) g的健康草鱼随机为高脂组和基础组,分别投喂含8.1%脂肪的高脂饲料和4.6%脂肪的基础饲料,并在试验的第4、8、12周检测草鱼血清中甘油三酯(TG)、总胆固醇(CHO)含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)活性以及肝胰脏中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性。在试验第12周,制作病理切片观察肝胰脏组织形态,并应用半定量反转录聚合酶链式反应(RT-PCR)检测肝胰脏ACC1 mRNA的相对表达量。结果表明:试验期间,高脂组草鱼肝细胞出现损伤,并随投喂时间的延长而加重;高脂组草鱼血清中AST、ALT活性及TG、CHO含量以及肝胰脏中MDA含量均随投喂时间的延长而显著或极显著上升(P＜0.05或P＜0.01),而肝胰脏中SOD和CAT活性则随投喂时间的延长而显著或极显著下降(P＜0.05或P＜0.01)。在试验第12周,高脂组草鱼血清中AST、ALT活性及TG、CHO含量以及肝胰脏中MDA含量均显著或极显著高于基础组(P＜0.05或P＜0.01),而肝胰脏中SOD和CAT活性则显著或极显著低于基础组(P＜0.05或P＜0.01);与基础组相比,高脂组草鱼肝胰脏ACC1 mRNA相对表达量极显著升高(P＜0.01)。由此得出,高脂饲料的连续投喂升高了草鱼的血脂水平,并使草鱼肝胰脏出现损伤,其作用机制可能与高脂饲料降低草鱼抗氧化能力以及促进肝脏脂肪合成有关。

关键词： 草鱼; 高脂饲料; 生化指标; ACC1 mRNA; 半定量RT-PCR

本文引用格式

汪开毓 , 苗常鸿 , 黄锦炉 , 王均 , 连海 , 吴春艳 , 牟巧凤 , 付希 . 投喂高脂饲料后草鱼主要生化指标和乙酰辅酶A 羧化酶1 mRNA表达的变化[J]. 动物营养学报, 2012 , 24(12) : 2375 -2383 . DOI: 10.3969/j.issn.1006-267x.2012.12.012

Abstract

The effects of consecutive feeding 8.1% fat diet for 12 weeks on serum biochemical indices, hepatopancreas biochemical indices and acetyl-CoA carboxylase 1 (ACC1) mRNA expression of grass carp were investigated to study the metabolic regulation mechanism of grass carp liver on high-fat diet. A total of 120 grass carp with an average body weight of (15.0±2.4) g were randomly divided into high-fat group and basal group, and the fish in those 2 groups were fed the diets containing 8.1% (high-fat diet) and 4.6% fat (basal diet), respectively. The contents of triglyceride (TG) and total cholesterol (CHO) and the activities of alanine aminotransferase (AST) and aspartate aminotransferase (ALT) in serum, and the content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and catalase (CAT) in hepatopancreas were determined on the 4th, 8th and 12th weeks, respectively. On the 12th week, the tissue morphology of hepatopancreas was observed by making pathological sections, and the relative expression of ACC1 mRNA in hepatopancreas was analyzed by semi-quantitative RT-PCR. The results showed as follows: in the experimental period, the hepatocytes of grass carp of high-fat group appeared injuries, and the injuries were more and more serious with increasing the feeding time; the contents of TG and CHO and the activities of AST and ALT in serum and the content of MDA in hepatopancreas of high-fat group were significantly increased (P＜0.05 or P＜0.01), while the activities of SOD and CAT in hepatopancreas of high-fat group were significantly decreased with increasing the feeding time (P＜0.05 or P＜0.01). On the 12th week, the contents of TG and CHO and the activities of AST and ALT in serum and the content of MDA in hepatopancreas of high-fat group were significantly higher than those of basal group (P<0.05 or P＜0.01), whereas the activities of SOD and CAT in hepatopancreas of high-fat group were significantly lower than those of basal group (P<0.05 or P＜0.01). Compared with the basal group, the relative expression of ACC1 mRNA of high-fat group was significantly increased on the 12th week (P<0.01). The results indicate that consecutive feeding high-fat diet can increase the blood fat level and damage the hepatopancreas of grass carp, and its mechanism may be related to the high-fat diet decreasing antioxidant ability and increasing liver fat synthesis of grass carp.

Key words： grass carp; high-fat diet; biochemical indices; ACC1 mRNA; semi-quantitative RT-PCR

参考文献

[1] 廖翔华,林鼎.养殖鱼类营养需求研究进展[J].水生生物学报,1989,13(2):170-186.
[2] 刘珂珂,王华朗.草鱼饲料与营养需求研究进展[J].广东饲料,2008,17(3):36-38.
[3] 雍文岳,黄忠志,廖朝兴,等.饲料中脂肪含量对草鱼生长的影响[J].淡水渔业,1985(6):11-14.
[4] DU Z Y,CLOUET P,DEGRACE P,et al.Hypolipidaemic effects of fenofibrate and fasting in the herbivorous grass carp (Ctenopharyngodon idella) fed a high-fat diet[J].British Journal of Nutrition,2008,100(6):1200-1212.  
[5] DU Z Y,LIU Y J,TIAN L X,et al.Effect of dietary lipid level on growth,feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella)[J].Aquaculture Nutrition,2005,11(2):139-146.  
[6] DU Z Y,CLOUET P,ZHENG W H,et al.Biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (Ctenopharyngodon idella) fed high-fat diets[J].British Journal of Nutrition,2006,95(5):905-915.  
[7] CHENG H,JI N,PENG Y,et al.Molecular characterization and tissue-specific expression of the acetyl-CoA carboxylase α gene from grass carp,Ctenopharyngodon idella[J].Gene,2011,487:46-51.
[8] BARBER M C,TRAVERS M T.Cloning and characterisation of multiple acetyl-CoA carboxylase transcripts in ovine adipose tissue[J].Gene,1995,154(2):271-275.  
[9] 程汉良,夏德全,吴婷婷.鱼类脂类代谢调控与脂肪肝[J].动物营养学报,2006,18(4):294-298.
[10] 王兴强,段青源,麦康森,等.养殖鱼类脂肪肝研究概况[J].海洋科学,2002,26(7):36-39.
[11] 金芬芬,徐团,秦圣娟,等.镉对长江华溪蟹肝胰腺线粒体抗氧化酶活力和脂质过氧化水平的影响[J].水生生物学报,2001,35(6):1019-1023.
[12] 蒋永芳,旷晖峰.慢性乙型病毒性肝炎患者血清 HBV DNA水平和谷丙转氨酶与肝组织病理的关系[J].重庆医科大学学报,2005,30(4):578-580.
[13] OLIVIRA C P M S,DA COSTA GAYOTTO L C,TATAI C,et al.Oxidative stress in the pathogenesis of nonalcoholic fatty liver disease,in rats fed with a choline-deficient diet[J].Journal of Cellular and Molecular Medicine,2002,6(3):399-406.  
[14] WOLF J,WLOFE M.A brief overview of nonneoplastic hepatic toxicity in fish[J].Toxicologic Pathology,2005,33(1):75-85.  
[15] GRATTAGLIANO I,VENDEMIALE G,CARACENI P,et al.Starvation impairs antioxidant defense in fatty livers of rats fed a choline-deficient diet[J].The Journal of Nutrition,2000,130(9):2131-2136.
[16] 岳颖,刘国华,郑爱娟,等.生长动物脂肪代谢关键酶基因表达调控[J].动物营养学报,2012,24(2):232-238.
[17] O’CALLAGHAN B L,KOO S H,WU Y,et al.Glucose regulation of the acetyl-CoA carboxylase promoter PI in rat hepatocytes[J].Journal of Biological Chemistry,2001,276(19):16033-16039.  
[18] MATSUMOTO T,TERAI S,OISHI T,et al.Medaka as a model for human nonalcoholic steatohepatitis[J].Disease Models & Mechanisms,2010,3(7/8):431-440.
[19] KUWASHIRO S,TERAI S,OISHI T,et al.Telmisartan improves nonalcoholic steatohepatitis in medaka (Oryzias latipes) by reducing macrophage infiltration and fat accumulation[J].Cell and Tissue Research,2011,344(1):125-134.  
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