刺五加多糖对断奶仔猪血液免疫指标的影响

韩杰; 边连全; 刘显军; 张飞; 杨侃侃

doi:10.3969/j.issn.1006-267x.2012.12.019

动物营养学报 >

2012 , Vol. 24 >Issue 12: 2444 - 2449

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.12.019

饲料营养 Feed Science and Technology

刺五加多糖对断奶仔猪血液免疫指标的影响

韩杰 ,
边连全 ,
刘显军 ,
张飞 ,
杨侃侃

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1. 沈阳农业大学畜牧兽医学院,沈阳 110866;
2. 洛阳师范学院生命科学系, 洛阳 471022;
3. 沈阳农业大学科研种猪场,沈阳 110866

收稿日期: 2012-06-11

网络出版日期: 2012-12-03

基金资助

国家"十二五"科技支撑计划项目(2011BAD28B01-02);科技部科技成果转化项目(2009GB2B000068)

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Effects of Dietary Acanthopanax senticosus Polysaccharide on Immune Parameters in Blood of Weaner Piglets

HAN Jie ,
BIAN Lianquan ,
LIU Xianjun ,
ZHANG Fei ,
YANG Kankan

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1. College of Animal Husbandry and Veterinary, Shenyang Agricultural University, Shenyang 110866, China;
2. Department of Life Science, Luoyang Normal University, Luoyang 471022, China;
3. Breeding Pigs Field for Scientific Research, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2012-06-11

Online published: 2012-12-03

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摘要

本试验旨在探讨刺五加多糖(Acanthopanax senticosus polysaccharide,ASPS)对断奶仔猪血液免疫指标的影响。试验选用96头(28±3)日龄的"长白×约克夏"二元杂交断奶仔猪,随机分成6组(每组4个重复,每个重复4头猪)。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中分别添加150、300、500、800和1 000 mg/kg ASPS的试验饲粮,试验期21 d。结果表明:饲粮中添加ASPS对仔猪外周血红细胞数量和中性粒细胞数量无显著影响(P＞0.05),但能显著增加外周血白细胞数量和淋巴细胞数量(P＜0.05),且随ASPS添加水平增加,白细胞数量和淋巴细胞数量呈二次曲线变化(P＜0.01)。添加ASPS能显著提高仔猪血清细胞因子白细胞介素-2(IL-2)和干扰素-γ(IFN-γ)含量(P＜0.05),对白细胞介素-4含量无显著影响(P＞0.05),且随ASPS添加水平增加,IL-2(P＜0.05)和IFN-γ含量(P＜0.01)呈二次曲线变化。ASPS对仔猪血清免疫球蛋白G、免疫球蛋白A、免疫球蛋白M抗体水平均无显著影响(P＞0.05)。ASPS对仔猪外周血白细胞数量和淋巴细胞数量及血清IL-2和IFN-γ含量的影响存在剂量依赖关系,500~1 000 mg/kg的ASPS能显著(P＜0.05)或极显著(P＜0.01)提高仔猪外周血白细胞数量、淋巴细胞数量,促进IFN-γ分泌,800 mg/kg的ASPS能显著促进IL-2分泌(P＜0.05),而当剂量低于500 mg/kg时,这种影响效果不显著(P＞0.05)。由此可见,ASPS可剂量依赖性地提高断奶仔猪外周血白细胞数量和淋巴细胞数量,增加血清细胞因子IL-2、IFN-γ的分泌,提高仔猪的免疫功能。

关键词： 刺五加多糖; 断奶仔猪; 免疫功能; 细胞因子

本文引用格式

韩杰 , 边连全 , 刘显军 , 张飞 , 杨侃侃 . 刺五加多糖对断奶仔猪血液免疫指标的影响[J]. 动物营养学报, 2012 , 24(12) : 2444 -2449 . DOI: 10.3969/j.issn.1006-267x.2012.12.019

Abstract

This experiment was conducted to investigate the effects of dietary Acanthopanax senticosus polysaccharide (ASPS) on blood immune parameters of weaner piglets. This experiment was a 21-d growth assay, in which 96 'Landrac×Yorkshire’ crossbred weaner piglets aged (28±3) days were randomly allotted to 6 dietary treatments supplemented with 0, 150, 300, 500, 800 and 1 000 mg/kg of ASPS, respectively. Each treatment was replicated in 4 pens containing 4 pigs per pen. The results showed that compared with the control, diets supplemented with ASPS did not significantly affect the number of red blood cell (RBC) and neutrophil in peripheral blood, the content of IL-4 and the antibody levels of IgG, IgA and IgM in serum (P＞0.05), but significantly increased the number of white blood cell (WBC) and lymphocyte in peripheral blood and the contents of IL-2 and IFN-γ in serum (P＜0.05). With the increase of dietary ASPS supplemental level, the number of WBC (P＜0.01) and lymphocyte in peripheral blood (P＜0.01), and serum IL-2 (P＜0.05) and IFN-γ contents (P＜0.01) were increased quadratically. The effects of dietary ASPS on the number of WBC and lymphocyte in peripheral blood and the contents of IL-2 and IFN-γ in serum had a dose-dependent relationship, 500 to 1 000 mg/kg ASPS significantly affected them (P＜0.05 or P＜0.01) except that serum IL-2 content was only affected by 800 mg/kg ASPS (P＜0.05), while the effects were not significant when the supplemental level was less than 500 mg/kg (P＞0.05). The results indicate that the number of WBC and lymphocyte in peripheral blood, and the contents of IL-2 and IFN-γ in serum are improved in a dose-dependent manner, and as a consequence, ASPS can enhance immunological function of weaner piglets.

Key words： Acanthopanax senticosus polysaccharide; weaner piglets; immune function; cytokines

参考文献

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