本试验旨在研究天冬酰胺(ASN)对脂多糖(LPS)刺激断奶仔猪生长性能、血细胞分类计数和血液生化指标的影响。选择24头"杜×长×大"断奶仔猪,随机分为4个组,每组6个重复,每个重复1头猪。4个组分别为:对照组(基础饲粮);LPS组(基础饲粮+LPS);0.5%ASN组(基础饲粮+LPS+0.5%ASN);1.0%ASN组(基础饲粮+LPS+1.0%ASN)。试验第16天,分别给LPS组、0.5%ASN组和1.0%ASN组的猪注射100 μg/kg BW的LPS,对照组注射等量的生理盐水。结果表明:1)0.5%ASN组可显著缓解LPS导致的平均日增重的下降(P<0.05)。2)在4 h时,0.5%ASN组显著提高了淋巴细胞和单核细胞比例(P<0.05);1.0%ASN组显著缓解了LPS刺激导致的嗜酸性粒细胞比例的升高和血小板数量的降低(P<0.05)。在24 h时,0.5%ASN组显著缓解了LPS刺激导致的红细胞数量和红细胞压积的降低(P<0.05);1.0%ASN组显著缓解了LPS刺激导致的白细胞和嗜中性粒细胞数量的上升和淋巴细胞数量、淋巴细胞比例、血红蛋白含量及红细胞压积的下降(P<0.05)。3)在24 h时,0.5%ASN组显著缓解了LPS刺激导致的谷草转氨酶(GOT)活性的上升和葡萄糖含量的下降(P<0.05);1.0%ASN组显著缓解了LPS刺激导致的GOT活性的上升和谷丙转氨酶/谷草转氨酶值的下降(P<0.05),显著提高了总蛋白含量(P<0.05),显著降低了碱性磷酸酶活性(P<0.05)。由此可见,ASN能在一定程度上缓解LPS刺激所导致的断奶仔猪生长抑制和应激反应。
李爽
,
刘玉兰
,
石海峰
,
陈逢
,
朱惠玲
,
侯永清
,
丁斌鹰
,
皮定安
,
冷炜博
. 天冬酰胺对脂多糖刺激断奶仔猪生长性能、血细胞分类计数和血液生化指标的影响[J]. 动物营养学报, 2012
, 24(12)
: 2450
-2458
.
DOI: 10.3969/j.issn.1006-267x.2012.12.020
This study was conducted to investigate the effects of asparagine (ASN) on growth performance, blood cell differential count and blood biochemical indices of weaner pigs challenged with lipopolysaccharide (LPS). Twenty-four weaner piglets (Duroc×Landrace×Large) were randomly allocated into 4 groups with 6 replicates and 1 piglet per replicate. The four groups included control group (basal diet), LPS group (basal diet+LPS), 0.5%ASN group (basal diet+LPS+0.5%ASN) and 1.0%ASN group (basal diet+LPS+1.0%ASN). On day 16, the piglets in the LPS group, 0.5%ASN group and 1.0%ASN group were injected intraperitoneally with 100 μg/kg BW LPS, and the piglets in the control group were injected intraperitoneally with the same dose of physiological saline. The results showed as follows: 1) 0.5%ASN group significantly alleviated the decrease of average daily gain (ADG) induced by LPS (P<0.05). 2) At 4 h, 0.5%ASN group significantly increased the percentage of lymphocytes and monocytes (P<0.05), and 1.0%ASN group significantly alleviated the increase of eosinophilic granulocyte percentage and the decrease of platelet number induced by LPS (P<0.05). At 24 h, 0.5%ASN alleviated the decrease of red blood cell number and hematokrit induced by LPS (P<0.05). 1.0%ASN group significantly alleviated the increase of the number of white blood cell and neutrophili induced by LPS (P<0.05), also significantly alleviated the decrease of lymphocyte number and percentage, hemoglobin content and hematocrit induced by LPS (P<0.05). 3) At 24 h, 0.5%ASN group significantly alleviated the increase of glutamic oxalacetic transaminase (GOT) activity and the decrease of blood glucose content induced by LPS (P<0.05). 1.0%ASN group significantly alleviated the increase of GOT and the decrease of glutamic-pyruvic transaminase (GPT)/GOT value induced by LPS (P<0.05), significantly increased the total protein content, and decreased the alkaline phosphatase activity (P<0.05). In conclusion, ASN can alleviate the growth depression and stress response induced by LPS to some degree.
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