动物营养学报 >

2013 , Vol. 25 >Issue 2: 289 - 298

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.02.009

禽营养 Poultry Nutrition

饲粮维生素E水平对22～42日龄黄羽肉鸡生长性能、免疫功能和抗氧化能力的影响

  • 蒋守群 ,
  • 周桂莲 ,
  • 林映才 ,
  • 陈芳 ,
  • 洪平 ,
  • 阮栋
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  • 广东省农业科学院畜牧研究所,畜禽育种国家重点实验室,农业部动物营养与饲料(华南)重点实验室,广东省动物育种与营养公共实验室,广东省畜禽育种与营养研究重点实验室,广州 510640

收稿日期: 2012-08-20

  网络出版日期: 2013-01-22

基金资助

公益性行业(农业)科研专项(200903006),国家肉鸡产业技术体系项目(CARS-42)

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Effects of Dietary Vitamin E Level on Growth Performance, Immune Function and Antioxidant Capacity of Yellow-Feathered Broilers Aged from 22 to 42 Days

  • JIANG Shouqun ,
  • ZHOU Guilian ,
  • LIN Yingcai ,
  • CHEN Fang ,
  • HONG Ping ,
  • RUAN Dong
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  • Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2012-08-20

  Online published: 2013-01-22

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摘要

本试验旨在研究饲粮维生素E水平对22～42日龄岭南黄羽肉鸡生长性能、免疫功能和抗氧化能力的影响。选用1 440只22日龄岭南黄羽肉鸡公鸡,共设6个处理,每个处理6个重复,每个重复40只鸡。试验采用玉米淀粉-豆粕型基础饲粮,各处理间除饲粮维生素E水平不同外,其他营养水平均一致。处理1(空白对照组,维生素E含量为3.59 mg/kg)为基础饲粮,处理2～6分别在基础饲粮中添加5、10、20、40、80 mg/kg维生素E。试验期21 d。试鸡地面平养,自由采食颗粒料和自由饮水。结果表明:饲粮中添加维生素E有降低试鸡饲料采食量的趋势(P=0.051),但对平均日增重、饲料增重比、胴体品质、脾脏指数、胸腺指数均未产生显著影响(P>0.05)。饲粮添加80 mg/kg维生素E显著提高了法氏囊指数(P<0.05),添加10、20、40和80 mg/kg维生素E显著降低了血清肿瘤坏死因子-α(TNF-α)含量(P<0.05),添加5、10 mg/kg维生素E使试鸡血清谷胱甘肽过氧化物酶活性分别提高了16.93%和23.46%(P<0.05),添加20 mg/kg维生素E显著提高了血清总超氧化物歧化酶(T-SOD)活性(P<0.05)。饲粮添加5、10、40、80 mg/kg维生素E显著提高了肝脏中α-生育酚含量(P<0.05),添加各水平维生素E均显著降低了肝脏中丙二醛含量(P<0.05),添加10、20 mg/kg维生素E显著提高了试鸡肌肉中T-SOD活性(P<0.05)。试验结果提示,仅考虑生长性能,玉米-豆粕型饲粮中不需添加维生素E;综合各抗氧化指标,经方差分析和多重比较得到22～42日龄黄羽肉鸡饲粮维生素E适宜水平为13.59～23.59 mg/kg;以血清TNF-α含量、T-SOD活性为评定指标,经回归分析得出饲粮维生素E适宜水平分别为59.88和46.64 mg/kg。

关键词： 维生素E; 免疫功能; 抗氧化能力; 黄羽肉鸡

本文引用格式

蒋守群 , 周桂莲 , 林映才 , 陈芳 , 洪平 , 阮栋 . 饲粮维生素E水平对22～42日龄黄羽肉鸡生长性能、免疫功能和抗氧化能力的影响[J]. 动物营养学报, 2013 , 25(2) : 289 -298 . DOI: 10.3969/j.issn.1006-267x.2013.02.009

Abstract

This experiment was conducted to investigate the effects of dietary vitamin E level on growth performance, immune function and antioxidant capacity of Lingnan yellow-feathered broilers aged from 22 to 42 days. One thousand four hundred and forty 22-day-old Lingnan yellow-feathered male broilers were randomly allocated to six treatments with six replicates per treatment and 40 birds in each replicate. Birds were fed the corn starch-soybean meal based diet (containing vitamin E 3.59 mg/kg) supplemented with vitamin E at the levels of 0 (control), 5, 10, 20, 40, or 80 mg/kg, and reared in indoor floor pens with ad libitum access to water and pellet feed for a period of 3 weeks. The nutrient levels of all diets were the same except vitamin E. The results showed that vitamin E supplementation tended to reduce average daily feed intake of broilers (P=0.051). There were no differences in average daily gain, feed to gain ratio, carcass traits, the indexes of spleen and thymus between treatments (P>0.05). Compared with the control group, adding 80 mg/kg vitamin E increased the index of Bursa of Fabricius (P<0.05), adding 10, 20, 40 and 80 mg/kg vitamin E decreased serum tumor necrosis factor-α (TNF-α) content (P<0.05), adding 5 and 10 mg/kg vitamin E increased serum glutathione peroxidase activity by 16.93% and 24.90% (P<0.05), respectively, and adding 20 mg/kg vitamin E increased serum total superoxide dismutase (T-SOD) activity(P<0.05). The supplementation of 5, 10, 40 and 80 mg/kg vitamin E increased hepatic α-tocopherol content (P<0.05), and adding vitamin E reduced malondialdehyde content (P<0.05). The supplementation of 10 and 20 mg/kg vitamin E elevated T-SOD activity in breast muscle (P<0.05). In conclusion, only considering growth performance of broilers, it is not necessary to supplement with vitamin E in corn-soybean meal based diets, but based on antioxidant parameters, the optimal vitamin E level estimated by variance analysis and multiple comparisons for broilers aged from 22 to 42 days is from 13.59 to 23.59 mg/kg, and based on serum TNF-α content and T-SOD activity, the optimal vitamin E levels estimated by regression analysis are 59.88 and 46.64 mg/kg, respectively.

Key words： vitamin E; immune function; antioxidant capacity; yellow-feathered broiler

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