本试验旨在探索提取板栗总苞多酚的最优工艺条件及研究其体外抗氧化能力。在单因素试验设计得出的提取条件的基础上,设计正交试验,确定最优提取工艺条件,并分别通过二苯代苦味酰基自由基(DPPH·)和2,2'-连氮-双-(3-乙基苯并噻唑啉-6-磺酸)自由基(ABTS+·)清除率以及猪油过氧化值(POV)的测定对板栗总苞多酚进行体外抗氧化性研究。结果表明:1)最优提取工艺条件:乙醇浓度为30%,液料比为18:1(mL:g),提取时间为140 min,提取温度为80 ℃。采用该工艺条件,板栗总苞多酚提取得率为22.61%,其中多酚含量为51.23%。2)板栗总苞多酚对DPPH·和ABTS+·清除率与维生素C相当,且均大于维生素E;板栗总苞多酚对猪油有良好的抗氧化效果,抗氧化能力与板栗总苞多酚的浓度有关,100 μg/mg为板栗总苞多酚对猪油抗氧化的最佳浓度,此浓度下板栗总苞多酚的抗氧化效果与同浓度的维生素C相当,且均优于同浓度的维生素E。因此,在本试验最优提取工艺条件下,板栗总苞多酚提取得率和多酚含量较高,且得到的板栗总苞多酚有良好的抗氧化能力。
The aims of this experiment were to optimize the extraction technology and to study the antioxidant activity of Castanea mollissima Blume polyphenols. The orthogonal test was adopted and the factors influencing the process parameters were determined. The antioxidant activity was evaluated through detecting DPPH· and ABTS+· scavenging rates, and peroxide value (POV) of Castanea mollissima Blume polyphenols in vitro. The results showed as follows: 1) optimal conditions for the extraction: ethanol concentration was 30%, liquid-solid ratio was 18:1 (mL:g), extraction temperature was 80 ℃, and extraction time was 140 min. The corresponding extraction yield was 22.61%, and the polyphenol content was 51.23%. 2) The DPPH· and ABTS+· scavenging rates of polyphenols from Castanea mollissima Blume were similar to that of vitamin C, and higher than that of vitamin E. Castanea mollissima Blume polyphenols displayed an excellent antioxidant activity in the lard oil and the effect depended on its concentration, with the best concentration of 100 μg/mg. The antioxidant effect of Castanea mollissima Blume polyphenols at this concentration was equal to that of vitamin C, and both of them were better than vitamin E. In conclusion, under the optimal conditions, Castanea mollissima Blume polyphenols shows higher extraction yield and content and reveals better antioxidant activity.
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