综述 Review

饲料中硫代葡萄糖苷、异硫氰酸酯和唑烷硫酮生物作用及其检测方法

  • 董晓芳 ,
  • 佟建明
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  • 中国农业科学院北京畜牧兽医研究所,北京 100193

收稿日期: 2012-09-07

  网络出版日期: 2013-02-28

基金资助

国家蛋鸡产业技术体系建设专项经费(CARS-41-K16)

Glucosinolates, Isothiocyanates and Vinylthiooxazolidone: Biological Function and Its Detection Method

  • DONG Xiaofang ,
  • TONG Jianming
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  • Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2012-09-07

  Online published: 2013-02-28

摘要

十字花科芸苔属植物,尤其是其种子饼粕是饲料中硫代葡萄糖苷的主要来源,不同浓度和结构的硫代葡萄糖苷降解产物可以产生抗营养作用、毒害作用、保健作用等完全不同的生物作用。欧盟和我国都对饲料中异硫氰酸酯和 唑烷硫酮的限量做了规定。针对异硫氰酸酯和 唑烷硫酮的检测方法很多,但欧盟并没有推荐标准的检测方法。我国饲料中异硫氰酸酯和 唑烷硫酮检测方法标准正在修订之中。因此,需要对硫代葡萄糖苷及其降解产物在动物中的生物作用进行重新认识和深入研究,并建立相应的检测方法标准,保障和促进油菜籽饼粕等十字花科植物及其副产物饲料的科学有效利用。本文旨在通过从饲料中硫代葡萄糖苷及其代谢产物异硫氰酸酯和 唑烷硫酮的来源、生物作用、限量规定、检测方法、方法标准方面进行分析和总结,从而对饲料中硫代葡萄糖苷、异硫氰酸酯和 唑烷硫酮的研究进展作一综述。

本文引用格式

董晓芳 , 佟建明 . 饲料中硫代葡萄糖苷、异硫氰酸酯和唑烷硫酮生物作用及其检测方法[J]. 动物营养学报, 2013 , 25(3) : 469 -478 . DOI: 10.3969/j.issn.1006-267x.2013.03.004

Abstract

The fodder and seed meals of genus Brassica are the chief source of glucosinolates in animal diets. Depending on the concentrations and structural types of these compounds, their biological effects can be toxic, anti-nutritional or beneficial to health. Maximum content of isothiocyanate and oxazolidinethione has been established in European Union (EU) and China. There are some methods detecting isothiocyanate and oxazolidinethione, but official method standard is not available in EU. The method standards of both isothiocyanate and oxazolidinethione are being amended in China. From present review it appears that it is necessary to revalue and further investigate the biological function of glucosinolates and its metabolites in animal and establish corresponding detection method standard to maintain and promote scientific and effective utilization of the plants and by-products of genus Brassica especially rapeseed meal in animal diets. This article given a brief introduction about the development on glucosinolates, isothiocyanates and oxazolidinethione in feed, the source and biological function, and regulations on limited use in feed, detection method and method standardization would analyzed and summarized on the basis of comprehensive literature.

参考文献

[1] European Commission.Opinion of the scientific panel on contaminants in the food chain on a request from the European commission on glucosinolates as undesirable substances in animal feed[J].The EFSA Journal,2008,590:1-76.
[2] FROHNE D,PFANDER H J.Poisonous plants:a handbook for doctors,pharmacists,toxicologists,biologists and veterinarians[M].London:Manson Publishing Ltd.,2005.
[3] KLIEBENSTEIN D J,KROYMANN J,MITCHELL O T.The glucosinolate-myrosinase system in an ecological and evolutionary context[J].Current Opinion in Plant Biology,2005,8(3):264-271.  
[4] TIAN Q,ROSELOT R A,SCHWARTZ S J.Quantitative determination of intact glucosinolates in broccoli,broccoli sprouts,brussels sprouts,and cauliflower by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry[J].Analytical Biochemistry,2005,343:93-99.
[5] FAHEY J W,ZALCMAN A T,TALALAY P.The chemical diversity and distribution of gluocosinolates and isothiocyanates among plants[J].Phytochemistry,2001,56(1):5-51
[6] HALKIER B A,GERSHENZON J.Biology and biochemistry of glucosinolates[J].Annual Review of Plant Biology,2006,57(1):303-333.  
[7] MATTHAUS B,ZUBR J.Variability of specific components in Camelina sativa oilseed cakes[J].Industrial Crops and Products,2000,12:9-18.
[8] RASK L,ANDREASSON E,EKBOM B,et al.Myrosinase:gene family evolution and herbivore defense in brassicaceae[J].Plant Molecular Biology,2000,42(1):93-114.  
[9] TRIPATHI M K,MISHRA A S.Glucosinolates in animal nutrition:a review[J].Animal Feed Science and Technology,2007,132:1-27.
[10] MARTLAND M F,BUTLER E J,FENWICK G R.Rapeseed induced liver hemorrhage reticulolysis and biochemical changes in laying hens:the effects of feeding high and low glucosinolate meals[J].Research in Veterinary Science,1984,63:298-309.
[11] SPIEGEL C,BESTERTTI G E,ROSSI G L,et al.Normal circulating triiodothyronine concentration are maintained despite severe hypothyroidism in growing pigs fed rapeseed presscake meal[J].The Journal of Nutrition,1993,123:1554-1561.
[12] TALJAARD T L.Cabbage poisoning in ruminants[J].Journal of South African Veterinary Association,1993,64:96-100.
[13] AHLIN K A,EMANUELSON M,WIKTORSON H.Rapeseed products from double-low cultivars as feed for dairy cows:effects of long term feeding on thyroid function,fertility and animal health[J].Acta Veterinaria Scandinavia,1994,35:37-53.
[14] SCHMID A,SCHMID H.Rapeseed poisoning of wild herbivores[J].Tierarzl Prax,1992,20:321-325.
[15] RODRIGUEZ R A,MACIEL M G,DE OCHOTECO M B.Blindness in Holando argentine calves due to ingestion of turnip (Brassica campestris)[J].Veterinario Argentina,1997,14:601-605.
[16] MAWSON R,HEANCY R K,ZDUNCZYK Z,et al.Rapeseed mealglucosinolates and their antinutritional effects. Part 3.Animal growth and performance[J].Nahrung,1994,38(2):167-177.  
[17] MAWSON R,HEANEY R K,ZDUNCZYK Z,et al.Rapeseed mealglucosinolates and their antinutritional effects.Part 4.Goitrogenicity and internal organs abnormalities in animals[J].Nahrung,1994,38(2):178-191.  
[18] MITHEN R F,DEKKER M,VERKERK R,et al.The nutritional significance,biosynthesis and bioavailability of glucosinolates in human food[J].Journal of the Science of Food and Agriculture,2000,80(7):967-984.  
[19] BUREL C,BOUJARD T,ESCAFFRE A M,et al.Dietary low-glucosinolate rapeseed meal affects thyroid status and nutrient utilization in rainbow trout (Oncorhynchus mykiss)[J].British Journal of Nutrition,2000,83:653-664.
[20] CONAWAY C C,YANG Y M,CHUNG F L.Isothiocyanates as cancer chemopreventive agents:their biological activities and metabolism in rodents and humans[J].Current Drug Metabolism,2002,3:233-255.
[21] CHUNG F L,KELLOFF G,STEELE V,et al.Chemopreventive efficacy of arylalkyl isothiocyanates and nacetylcysteine for lung tumorigenesis in Fischer rats[J].Cancer Research,1996,56:772-778.
[22] STONER G,CASTO B,RALSTON S,et al.Development of a multi-organ rat model for evaluating chemopreventive agents:efficacy of indole-3-carbinol[J].Carcinogenesis,2002,23:265-272.
[23] ADAM R G,MORSE M A,STONER G D.The effects of phenethyl isothiocyanate on benzo(a)pyrene-induced tumors and DNA adducts in A/J mouse lung[J].Cancer Letters,1993.71:35-42.
[24] National Toxicology Program.NTP carcinogenesis bioassay of allyl isothiocyanate (CAS No.57-06-7) in F344N rats and B6C3F1 mice (gavage study)[EB/OL].1982[2012-09-02].http://ntp.niehs.nih.gov/ntp/htdocs/LT_rpts/tr234.pdf.
[25] HIGDON J V,DELAGE B,WILLIAMS D E,et al.Cruciferous vegetables and human cancer risk:epidemiological evidence and mechanistic basis[J].Pharmacological Research,2007,55:224-236.
[26] BOURDON D,AUMAITRE A.Low-glucosinolate rapeseeds and rapeseed meals:effect of technological treatments on chemical composition,digestible energy content and feeding value for growing pigs[J].Animal Feed Science and Technology,1990,30:175-191.
[27] MABON N,JEAN P W,MICHEL M.Analysis of 5-viny-1,3-oxazolidine-2-thione in complex matrices at ppb level[J].Talanta,1999,49(1):199-206.  
[28] RONG T,QING Y,JOHN P,et al.Direct and simultaneous analysis of sinigrin and allyl isothiocyanate in mustard samples by high-performance liquid chromatography[J].Journal of Agricultural and Food Chemistry,2002,50(17):4749-4753.  
[29] HAN H,LI W,XIE F,et al.Improved monitoring methods for toxic metabolites of rapeseed meal[J].Chinese Journal of Pharmacology and Toxicology,2001,15(5):386-391.

[30] MATTHAUS B,FIEBIG H J.Simultaneous determination of isothiocyanates,indoles,and oxazolidinethiones in myrosinase digests of rapeseeds and rapeseed meal by HPLC[J].Journal of Agricultural and Food Chemistry,1996,44(12):3894-3899.  
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