饲料营养 Feed Science and Technology

约氏乳杆菌对产蛋鸡生产性能、蛋品质和免疫机能的影响

  • 鲍延娥 ,
  • 汪攀 ,
  • 董晓芳 ,
  • 王安如 ,
  • 佟建明 ,
  • 王少璞 ,
  • 张军 ,
  • 胡婷
展开
  • 1. 中国农业科学院北京畜牧兽医研究所,北京 100193;
    2. 北京大北农科技集团股份有限公司, 饲用微生物工程国家重点实验室,北京 100080

收稿日期: 2012-09-05

  网络出版日期: 2013-02-28

基金资助

国家蛋鸡产业技术体系建设专项经费(CARS-41-K16);"973"计划资助项目(2010CB35703)

Lactobacillus johnsonii: Effects on Performance, Egg Quality and Immune Function of Laying Hens

  • BAO Yan ,
  • WANG Pan ,
  • DONG Xiaofang ,
  • WANG Anru ,
  • TONG Jianming ,
  • WANG Shaopu ,
  • ZHANG Jun ,
  • HU Ting
Expand
  • 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. State Key Laboratory of Direct-Fed Microbial Engineering, Beijing Dabeinong Technology Group Co., Ltd., Beijing 100080, China

Received date: 2012-09-05

  Online published: 2013-02-28

摘要

本试验旨在研究饲粮中添加约氏乳杆菌对产蛋鸡生产性能、蛋品质和免疫机能的影响。试验选取体重和产蛋率相近的540只130日龄海兰褐产蛋鸡,随机分成6组(每组6个重复,每个重复15只):对照组饲喂基础饲粮,试验组饲喂在基础饲粮中分别添加1.0×104、1.0×105、1.0×106、1.0×107和1.0×108 CFU/g约氏乳杆菌的试验饲粮,试验期24周(168 d)。结果表明:1)饲粮中添加1.0×105 CFU/g约氏乳杆菌可以在一定程度上改善产蛋鸡产蛋率、平均蛋重、平均日采食量和料蛋比,其中对试验17~24周时的料蛋比影响显著(P<0.05);2)饲粮中添加1.0×104~1.0×108 CFU/g约氏乳杆菌对产蛋鸡蛋壳强度、蛋白高度和哈氏单位均无显著影响(P>0.05),但能够显著提高鸡蛋的蛋黄颜色(P<0.05);3)仅饲粮中添加1.0×106 CFU/g约氏乳杆菌能够提高试验第35天时的血清新城疫抗体效价(P<0.01),但与添加1.0×105、1.0×107和1.0×108 CFU/g约氏乳杆菌一样会降低试验第7天和第35天时的免疫球蛋白A水平(P<0.01),同时可提高试验第28天时的免疫球蛋白A水平(P<0.01)。结果提示,饲粮中添加适量的约氏乳杆菌能够改善产蛋鸡的生产性能和蛋品质,调节机体的免疫功能。

本文引用格式

鲍延娥 , 汪攀 , 董晓芳 , 王安如 , 佟建明 , 王少璞 , 张军 , 胡婷 . 约氏乳杆菌对产蛋鸡生产性能、蛋品质和免疫机能的影响[J]. 动物营养学报, 2013 , 25(3) : 595 -602 . DOI: 10.3969/j.issn.1006-267x.2013.03.018

Abstract

This experiment was conducted to study the effects of dietary Lactobacillus johnsonni (L. johnsonni) on performance, egg quality and immune function of laying hens. Five hundred and forty Hy-Line brown laying hens aged 130 days were randomly allocated into 6 groups with 6 replicates per group and 15 birds per replicate. Laying hens in control group were fed a basal diet, and those in trial groups were fed the basal diet supplemented with 1.0×104, 1.0×105, 1.0×106, 1.0×107 and 1.0×108 CFU/g L. johnsoni, respectively. The experiment lasted for 24 weeks (168 days). The results showed as follows: 1) the laying rate, average egg weight, average daily feed intake and feed/egg in 1.0×105 CFU/g L. johnsonii group were improved to a certain extent, in which feed/egg at 17 to 24 weeks of the experiment was significantly affected (P<0.05); 2) dietary 1.0×104 to 1.0×108 CFU/g L. johnsonii significantly increased the yolk color (P<0.05), but the shell strength, albumen height and Haugh unit were not significantly affected (P>0.05); 3) only 1.0×106 CFU/g L. johnsonii significantly increased the antibody titer to Newcastle disease on day 35 (P<0.01), but reduced the IgA level on days 7 and 35 and increased IgA level on day 28 like the supplementation of 1.0×105, 1.0×107 and 1.0×108 CFU/g L. johnsonii (P<0.01). It is concluded that dietary appropriate L. johnsonii can improve the performance and egg quality, and regulate the immunological function of laying hens.

参考文献

[1] GEIER M S,MIKKELSEN L L,TOROK V A,et al.Comparison of alternatives to in-feed antimicrobials for the prevention of clinical necrotic enteritis[J].Journal of Applied Microbiology,2010,109(4):1329-1338.  
[2] 倪学琴,曹西亮,曾东,等.猪源约氏乳酸杆菌JJB3和枯草芽孢杆菌JS01对仔猪肠道分泌型免疫球蛋白A和菌群的影响[J].动物营养学报,2008,20(3):275-280.
[3] KINGMA S D,LI N,SUN F,et al.Lactobacillus johnsonii N6.2 stimulates the innate immune response through Toll-like receptor 9 in Caco-2 cells and increases intestinal crypt Paneth cell number in biobreeding diabetes-prone rats[J].The Journal of Nutrition,2011,141(6):1023-1028.  
[4] INOUE R,NISHIO A,FUKUSHIMA Y,et al.Oral treatment with probiotic Lactobacillus johnsonii NCC533 (La1) for a specific part of the weaning period prevents the development of atopic dermatitis induced after maturation in model mice,NC/Nga[J].British Journal of Dermatology,2007,156(3):499-509.  
[5] YANG J,LI W,SUN R,LI B,et al.The effect of Lactobacillus johnsonii Ncc533 (La1) on the balance of Th1/Th2 cells in BALB/c mice[J].Clinical and Investigative Medicine,2011,34(4):E254.
[6] JOO H M,HYUN Y J,MYOUNG K S,et al.Lactobacillus johnsonii HY7042 ameliorates Gardnerella vaginalis-induced vaginosis by killing Gardnerella vaginalis and inhibiting NF-κB activation[J].International Immunopharmacology,2011,11(11):1758-1765.  
[7] HALLER D,BODE C,HAMMES W P,et al.Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures[J].Gut,2000,47(1):79-87.  
[8] EFSA.Scientific opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2010 update)[J].European Food Safety Authority (EFSA) Journal,2010,8(12):1944.
[9] TAHERI H R,MORAVEJ H,TABANDEH F,et al.Efficacy of combined or single use of Lactobacillus crispatus LT116 and L.johnsonii LT171 on broiler performance[J].British Poultry Science,2010,51(5):580-585.  
[10] 倪学琴.约氏乳酸杆菌JJB3和纳米硒对断奶仔猪生产性能、肠道菌群结构及多样性的影响[D].硕士毕业论文.成都:四川农业大学,2007.
[11] ABE F,ISHIBASHI N,SHIMAMURA S.Effect of administration of bifidobacteria and lactic acid bacteria to newborn calves and piglets[J].Journal of Dairy Science,1995,78(12):2838-2346.  
[12] MAHDAVI A H,RAHMANI H R,POURREZA J.Effect of probiotic supplements on egg quality and laying hen’s performance[J].International Journal of Poultry Science,2005,4(7):488-492.  
[13] MILES R D,ARAFA A S,HARMS R H,et al.Effects of a living nonfreeze-dried Lactobacillus acidophilus culture on performance,egg quality,and gut microflora in commercial layers[J].Poultry Science,1981,60:993-1004.
[14] PRIDMORE R D,PITTET A C,PRAPLAN F,et al.Hydrogen peroxide production by Lactobacillus johnsonii NCC 533 and its role in anti-Salmonella activity[J].Federation of European Microbiological Societies,2008,283(2):210-215.
[15] 章文明,汪海峰,刘建新,等.乳酸杆菌益生作用机制的研究进展[J].动物营养学报,2012,24(3):389-396.
[16] TAHERI H,TABANDEH F,MORAVEJ H,et al.Potential probiotic of Lactobacillus johnsonii LT171 for chicken nutrition[J].African Journal of Biotechnology,2011,21:5833-5837.
[17] NIKOSKELAINEN S,SALMINEN S,BYLUND G,et al.Characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish[J].Applied and Environmental Microbiology,2001,67:2430-2435.
[18] 张力,郑中朝.饲料添加剂手册[M].北京:化学工业出版社,2000.
[19] 贾卿,徐斌龙,白子金,等.嗜酸乳杆菌对蛋鸡生产性能、蛋品质和血液生化指标的影响[C]//中国畜牧兽医学会2011学术年会论文集.北京:中国畜牧兽医学会,2011:632.
[20] PANDA A K,RAMA-RAO S S,RAJU M V,et al.Effect of probiotic (Lactobacillus sporogenes) feeding on egg production and quality,yolk cholesterol and humoral immune response of White Leghorn layer breeders[J].Journal of the Science of Food and Agriculture,2007,88:43-47.
[21] GALLAZZI D,GIARDINI A,MANGIAGALLI M G,et al.Effects of Lactobacillus acidophilus D2/CSL on laying hen performance[J].The Italian Journal of Animal Science,2008,7(1):27-37.
[22] AGHAII A,CHAJI M,MOHAMMADABADI T,et al.The effect of probiotic supplementation on production performance,egg quality and serum and egg chemical composition of laying hens[J].Journal of Animal and Veterinary Advances,2010,21(9):2774-2777.
[23] 李士平.蛋黄着色影响因素及着色剂的研究[D].硕士学位论文.哈尔滨:东北农业大学,2000.
[24] TRABER M G,DIAMOND S R,LANE J C,et al.Beta-carotene transport in human lipoproteins.Comparisons with a-tocopherol[J].Lipids,1994,29(10):665-669.  
[25] MCCRACKEN V J,GASKINS H R.Probiotics and the immune system[M]//TANNOCK G W.Probiotics:a critical review.Hethersett:Horizon Scientific Press,1999:85-112.
[26] OUWEHAND A C,ISOLAURI E,KIRJAVAINEN P V,et al.The mucus binding of Bifidobacterium lactis Bb12 is enhanced in the presence of Lactobacillus GG and Lact.delbrueckii subsp.bulgaricus[J].Letters in Applied Microbiology,2010,30:10-13.
[27] BERGONZELLI G E,GRANATO D.GroEL of Lactobacillus johnsonii La1 (NCC 533) is cell surface associated:potential role in interactions with the host and the gastric pathogen Helicobacter pylori[J].Infection and Immunity,2006,74:425-434.
[28] LINK-AMSTER H,ROCHAT F,SAUDAN K Y,et al.Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented milk intake[J].FEMS Immunology and Medical Microbiology,1994,10(1):55-63.  
[29] 曹希亮.枯草芽孢杆菌和约氏乳杆菌对仔猪T细胞免疫与肠道黏膜免疫的影响[D].硕士学位论文.成都:四川农业大学,2007.
[30] SGOURAS D N,PANAYOTOPOULOU E G,MARTINEZ-GONZALEZ B,et al.Lactobacillus johnsonii La1 attenuates Helicobacter pylori-associated gastritis and reduces levels of proinflammatory chemokines in C57BL/6 mice[J].Clinical and Diagnostic Laboratory Immunology,2005,12(12):1378-1386.
[31] KABURAGI T,YAMANO T,FUKUSHIMA Y,et al.Effect of Lactobacillus johnsonii La1 on immune function and serum albumin in aged and malnourished aged mice[J].Nutrition,2007,23(4):342-350.  
[32] GRANATO D,BERGONZELLI G E,PRIDMORE R D,et al.Cell surface-associated elongation factor Tu mediates the attachment of Lactobacillus johnsonii NCC533 (La1) to human intestinal cells and mucins[J].Infection and Immunity,2004,72:2160-2169.
[33] SCHIFFRIN E J,ROCHAT F,LINKAMSTER H,et al.Immunomodulation of human blood cells following the ingestion of lactic acid bacteria[J].Journal of Dairy Science,1995,78(3):491-497.  
文章导航

/