为优化牛前体脂肪细胞培养方法,本试验通过观察显微镜下细胞形态、噻唑蓝(MTT)法、油红O染色法和RNA质量检测结果,比较组织块培养法、传统消化法和改良消化法培养的牛前体脂肪细胞增殖分化的差异。结果表明:3种方法均能培养出成熟脂肪细胞,细胞形态一致,其中改良消化法极大地缩短了试验样品处理的时间,且细胞形态最好,提取的RNA质量最好。改良消化法是一种快捷、有效的牛前体脂肪细胞培养方法。
In order to optimize the method of bovine preadipocytes culture, proliferation and differentiation of cells cultured by methods of tissue culture, traditional digestion culture and modified digestion culture were studied and compared using results of cell growth observation under microscope, MTT method, oil red O staining and RNA quality detection. The results showed that adipocytes were successfully cultured by the three methods, and the cells were highly homogenous. Modified digestion culture significantly shorted the culture time, and cell morphology and RNA extraction quality were the best. Therefore, modified digestion culture is a rapid and efficient method for bovine preadipocytes culture.
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