综述 Review

木聚糖酶基因的体外定向进化

  • 杜文 ,
  • 王谦 ,
  • 王佳堃 ,
  • 刘建新
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  • 浙江大学奶业科学研究所, 动物分子营养学教育部重点实验室, 杭州 310058

收稿日期: 2013-04-03

  网络出版日期: 2013-09-16

基金资助

国际原子能合作项目(RC-16327-R0)

Directed Evolution of Xylanase Genes in Vitro

  • DU Wen ,
  • WANG Qian ,
  • WANG Jiakun ,
  • LIU Jianxin
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  • Key Laboratory of Molecular Animal Nutrition of Ministry of Education, Institute of Dairy Science, Zhejiang University, HangZhou 310058, China

Received date: 2013-04-03

  Online published: 2013-09-16

摘要

木聚糖酶在天然材料中基因表达水平低、活性差、生产成本高,严重限制了它的推广应用。宏基因组学通过免培养技术,研究生境中全部微小生物遗传物质的总和,在开发微生物酶资源上具有强劲的优势。体外定向进化技术模拟达尔文的自然进化论,利用基因的突变和重组,从体外改造酶基因,产生基因多样性,并结合定向的筛选最终获得预期性状的进化酶。宏基因组技术与体外定向进化技术结合必将加速木聚糖酶的开发和产业化应用,推动半纤维类生物能源的利用。为此,本文就木聚糖酶基因的来源、宏基因组学在木聚糖酶基因开发中的应用、体外定向进化进行了系统的综述。

本文引用格式

杜文 , 王谦 , 王佳堃 , 刘建新 . 木聚糖酶基因的体外定向进化[J]. 动物营养学报, 2013 , 25(10) : 2202 -2211 . DOI: 10.3969/j.issn.1006-267x.2013.10.003

Abstract

The application of xylanases is limited by certain factors, such as low gene expression level, low enzyme activity and high cost. Metagenomics study the genetic materials of communities of microbial organisms in their natural environments by culture-independent technology, which showd great potential in exploring the resource of enzymes. Directed evolution, which simulates natural evolution of Darwin, reforms enzyme genes and creats gene diversity by gene mutation and recombination in vitro, and gets superior enzymes with expected properties by directed screening. The combination of metagenomics and directed evolution will accelerate the exploitation and industrial application of xylanases, promote the utilization of hemicellulose energy. Therefore, the sources of xylanase genes, the application of metagenomics in exploiting xylanases and directed evolution in vitro were reviewed in this article.

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