动物营养学报 >

2013 , Vol. 25 >Issue 10: 2246 - 2253

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.10.008

禽营养 Poultry Nutrition

相对湿度和氨气应激对肉仔鸡血氨水平及细胞因子含量的影响

  • 魏凤仙 ,
  • 胡骁飞 ,
  • 张敏红 ,
  • 李绍钰 ,
  • 徐彬 ,
  • 蔺萍 ,
  • 孙全友 ,
  • 李浩
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  • 1. 河南省农业科学院畜牧兽医研究所, 郑州 450002;
    2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    3. 河南农业科学院, 河南省动物免疫学重点实验室, 郑州 450002;
    4. 河南省森林航空消防站, 郑州 450003

收稿日期: 2013-04-23

  网络出版日期: 2013-09-16

基金资助

动物营养学国家重点实验室开放课题(2004DA125184F1310);国家现代农业产业技术体系项目(CARS-42);河南省重点科技攻关项目(102102110017)

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Effects of Relative Humidity and Ammonia Stress on Plasma Ammonia Level and Cytokine Contents of Broilers

  • WEI Fengxian ,
  • HU Xiaofei ,
  • ZHANG Minhong ,
  • LI Shaoyu ,
  • XU Bin ,
  • LIN Ping ,
  • SUN Quanyou ,
  • LI Hao
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  • 1. Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
    2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, The Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. The Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
    4. Henan Aerial Forest Fire Station, Zhengzhou 450003, China

Received date: 2013-04-23

  Online published: 2013-09-16

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摘要

本试验旨在研究禽舍环境不同相对湿度(RH)和氨气(NH3)应激对肉仔鸡血氨水平及细胞因子含量的影响。选择288只21日龄体重接近的肉仔鸡,随机分为6个处理,每个处理6个重复,每个重复8只鸡。处理1:30 mg/kg NH3+35% RH;处理2:30 mg/kg NH3+60% RH;处理3:30 mg/kg NH3+85% RH;处理4:70 mg/kg NH3+35% RH;处理5:70 mg/kg NH3+60% RH;处理6:70 mg/kg NH3+85% RH。试验在智能人工气候舱完成,试验期3周。结果表明:1)NH3浓度对血氨水平影响显著(P<0.05),血氨水平随入舱时间的延长而上升。2)RH对入舱3周血清白细胞介素1β(IL-1β)含量影响显著(P<0.05),随入舱时间的延长,血清IL-1β和白细胞介素6(IL-6)含量呈上升的趋势。3)NH3浓度对入舱1、2周血清白细胞介素4(IL-4)含量影响显著(P<0.05),对入舱1、3周血清白细胞介素10(IL-10)含量影响显著(P<0.05)。RH对入舱1周血清IL-10含量影响显著(P<0.05)。由此可见,RH和NH3应激损害了肉仔鸡免疫机能,随着入舱时间的延长,机体获得一定的适应不良环境的能力,对机体的免疫抑制效应具有降低趋势,但70 mg/kg NH3+85% RH对肉仔鸡的免疫抑制不可缓解。

关键词： 相对湿度; NH3; 肉仔鸡; 血氨; 细胞因子

本文引用格式

魏凤仙 , 胡骁飞 , 张敏红 , 李绍钰 , 徐彬 , 蔺萍 , 孙全友 , 李浩 . 相对湿度和氨气应激对肉仔鸡血氨水平及细胞因子含量的影响[J]. 动物营养学报, 2013 , 25(10) : 2246 -2253 . DOI: 10.3969/j.issn.1006-267x.2013.10.008

Abstract

This experiment was conducted to study the effects of different relative humidity (RH) and ammonia (NH3) stress in poultry house on plasma ammonia level and cytokine contents of broilers. A total of 288 21-day-old broilers with an average bodyweight were divided into 6 treatments with 6 replicates per treatment and 8 broilers per replicate. The 6 treatments were as follows: Treatment 1, 30 mg/kg NH3+35% RH; treatment 2, 30 mg/kg NH3+60% RH, treatment 3, 30 mg/kg NH3+85% RH; treatment 4, 70 mg/kg NH3+35% RH; treatment 5, 70 mg/kg NH3+60% RH; treatment 6, 70 mg/kg NH3+85% RH. The experiment was conducted in climate chambers, and the experiment lasted for 3 weeks. The results showed as follows: 1) NH3 concentration had a significant influence on plasma ammonia level (P<0.05), the plasma ammonia level was increased with the increase of time in climate chambers. 2) RH had a significant influence on serum interleukin 1β (IL-1β) content at 3 weeks in climate chambers (P<0.05), the contents of IL-1β and interleukin 6 (IL-6) in serum were increased with the increase of time in climate chambers. 3) NH3 concentration had a significant influence on serum interleukin 4 (IL-4) content at 1 and 2 weeks in climate chambers (P<0.05), also had a significant influence on serum interleukin 10 (IL-10) content at 1 and 3 weeks in climate chambers (P<0.05). RH had a significant influence on serum IL-10 content at 1 week in climate chambers (P<0.05). It is concluded that RH and NH3 stress impair the immune function of broilers. With the increase of time in climate chambers, the body obtains certain ability to adapt to the environment, the immunosuppressive effects has a reduced tendency. Moreover, the immune suppression of broilers caused by 70 mg/kg NH3+85% RH cannot be alleviated.

Key words： relative humidity; NH3; broilers; plasma ammonia; cytokine

参考文献

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