本试验以DL-蛋氨酸作为对照,评估L-蛋氨酸作为断奶仔猪饲粮蛋氨酸来源的有效性,并初步确定满足断奶仔猪营养需要的标准回肠可消化蛋氨酸(SID Met)水平对断奶仔猪生长性能、营养物质表观消化率及血浆参数的影响。试验采用完全随机区组设计,将180头28日龄断奶的"杜×长×大"三元杂交仔猪[初始体重为(7.83±1.37) kg]按体重、性别、遗传基础相似的原则,分为5个组,每组6个重复,每个重复6头猪,公母分开饲养。对照组以DL-蛋氨酸作为蛋氨酸来源,SID Met水平为0.34%;试验组以L-蛋氨酸作为蛋氨酸来源,SID Met水平分别为0.28%(试验Ⅰ组)、0.31%(试验Ⅱ组)、0.34%(试验Ⅲ组,与对照组SID Met水平相同)和0.37%(试验Ⅳ组)。试验期28 d。结果表明:饲粮中SID Met水平的升高可显著提高平均日增重并显著改善饲料转化率(P<0.05),同时干物质和粗蛋白质的表观消化率呈线性升高(P<0.05);随着饲粮SID Met水平的提高,血浆尿素氮含量显著降低(P<0.05),血浆游离蛋氨酸和半胱氨酸含量显著增高而丝氨酸含量显著降低(P<0.05);根据折线模型,以平均日增重为指标,得出本试验条件下断奶仔猪适宜SID Met水平为0.35%。对照组与试验Ⅲ组相比,断奶仔猪的生长性能、营养物质表观消化率及血浆参数差异均不显著(P>0.05);以平均日增重为指标的相对生物学效价结果表明,等摩尔量L-蛋氨酸相对于DL-蛋氨酸的生物学效价为97%。由此可见,本试验使用的L-蛋氨酸可作为断奶仔猪饲粮的有效蛋氨酸来源,适宜SID Met水平为0.35%;初步评估L-蛋氨酸与DL-蛋氨酸相对生物学效价相当,二者在断奶仔猪饲粮中饲用效果相近。
This experiment was conducted to estimate the efficiency of L-methionine as a dietary methionine source using DL-methionine as a control, and investigate the effects of different dietary standardized ileal digestible methionine (SID Met) levels on growth performance, nutrient digestibility and plasma parameters of weaner piglets. A total of 180 weaner piglets were [initial body weight of (7.83±1.37) kg] allotted to five groups in a randomized complete block design. Each group had six replicates with six piglets per replicate. The control group was supplemented with DL-methionine as the methionine source, and dietary SID Met level was 0.34%. The experimental groups were added L-methionine as the methionine source, and the dietary SID Met levels were 0.28% (experimental group Ⅰ), 0.31% (experimental group Ⅱ), 0.34% (experimental group Ⅲ, the same as control group) and 0.37% (experimental group Ⅳ), respectively. The experiment lasted for 28 days. The results showed as follows: the increasing dietary SID Met level could significantly increase average daily gain and improve feed conversion ratio (P<0.05), and the apparent digestibility of dry matter and crude protein was significantly increased (P<0.05). The increasing dietary SID Met level significantly reduced the plasma urea nitrogen content (P<0.05), significantly increased the plasma methionine and cysteine contents (P<0.05) and significantly decreased the plasma serine content (P<0.05). Based on a straight broken-line model, the estimated optimum SID Met level was 0.35% for weaner piglets. No significant differences in growth performance, nutrient apparent digestibility and plasma parameters were observed between control group and experimental group Ⅲ. The relative bioefficacy of equimolar L-methionine to DL-methionine was 97% using average daily gain regressed on SID Met intake. In conclusion, L-methionine used in the current study is an available methionine source for weaner piglets, the optimum SID Met level is 0.35%; L-methionine has the equivalent bioefficacy compared with DL-methionine in weaner piglets diets.
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